Prostatic Acid Phosphatase抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 6293R
英文名称Prostatic Acid Phosphatase
中文名称前列腺酸性磷酸酶抗体
别 名Acid phosphatase prostate; ACP 3; ACP3; ACPP; Ecto-5'-nucleotidase; PAP; PPAP_HUMAN; Prostatic acid phosphatase; Thiamine monophosphatase; TMPase; 5'-NT; 5'-nucleotidase.
说 明 书100ul 200ul
研究领域肿瘤 细胞生物 激酶和磷酸酶 肿瘤细胞生物标志物
抗体来源Rabbit
克隆类型Polyclonal
Prostatic Acid Phosphatase抗体交叉反应 Human, Mouse, Rat, Dog, Horse,
产品应用WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量41kDa
细胞定位细胞膜 分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Prostatic Acid Phosphatase
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Prostatic Acid Phosphatase抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Prostatic Acid Phosphatase is Belongs to the histidine acid phosphatase family.Cellular localization;Secreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles.
Function:
A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.
Subunit:
Homodimer; dimer formation is required for phosphatase activity.
Subcellular Location:
Isoform 1: Secreted. Isoform 2: Cell membrane.
Isoform 2: the cellular form also has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor. Acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling.
Tissue Specificity:
Highly expressed in the prostate, restricted to glandular and ductal epithelial cells.
Post-translational modifications:
N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides.
Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection) which entrap HIV virions, attach them to target cells and enhance infection. SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea. Target cell attachment and enhancement of HIV infection is inhibited by surfen. Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection.
Similarity:
Belongs to the histidine acid phosphatase family.
SWISS:
P24666
Gene ID:
55
Prostatic Acid Phosphatase抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Prostatic Acid Phosphatase抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 Phospho-FGFR1+FGFR2 (Tyr463/Tyr466) 磷酸化碱性成纤维细胞生长因子受体1/2(Tyr463/Tyr466)抗体
合格 合格 Phospho-mTOR (Tyr144) 磷酸化雷帕霉素靶蛋白抗体
合格 Phospho-RhoA (Ser188) 磷酸化RhoA蛋白抗体
合格 合格 Phospho-mTOR (Thr2446) 磷酸化雷帕霉素靶蛋白抗体
合格 Phospho-ELk1(Thr417) 磷酸化细胞转录因子ELK1抗体
合格 Phospho-Estrogen Receptor alpha (Ser305) 磷酸化雌**受体α抗体
合格 phospho-FXYD1 (Ser83) 磷酸化磷酸神经膜抗体
合格 phospho-FXYD1 (Ser88) 磷酸化磷酸神经膜抗体
合格 合格 phospho-GFAP (Ser8) 磷酸化胶质纤维酸性蛋白抗体
合格 phospho-GABBR1 (Ser923) 磷酸化gamma氨基丁酸B型受体1抗体
合格 Phospho-Histone H2A.X (Tyr143) 磷酸化组蛋白H2AX抗体
合格 phospho-HSF1 (Ser303) 磷酸化热休克因子1抗体
合格 phospho-HSF1 (Ser307) 磷酸化热休克因子1抗体
合格 phospho-HSP70 (Tyr525) 磷酸化热休克蛋白70抗体
合格 phospho-GSK-3 Beta(Ser21+Ser29) 磷酸化糖原合酶激酶3β抗体
合格 phospho-GSK-3 Beta(Ser21) 磷酸化糖原合酶激酶3β抗体
合格 phospho-G3BP1(Ser232) 磷酸化Ras GTP酶活化蛋白结合蛋白1抗体