Proteasome 20S beta 6抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 9358R
英文名称Proteasome 20S beta 6
中文名称蛋白酶体PSMβ6抗体
别 名Delta; LMPY; Macropain delta chain; MGC5169; Multicatalytic endopeptidase complex delta chain; Proteasome (prosome macropain) subunit beta type 6; Proteasome beta 6 subunit; Proteasome catalytic subunit 1; Proteasome delta chain; Proteasome subunit beta 6; Proteasome subunit beta type 6; Proteasome subunit beta type-6; Proteasome subunit delta; Proteasome subunit Y; PSB6_HUMAN; PSMB 6; PSMB6; PSY large multifunctional protease Y; Y antibody.
说 明 书100ul 200ul
研究领域细胞生物 **学 细胞周期蛋白 细胞分化
抗体来源Rabbit
克隆类型Polyclonal
Proteasome 20S beta 6抗体交叉反应 Human, Mouse, Rat, Pig,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:400-800 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量22kDa
细胞定位细胞核 细胞浆 细胞外基质
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Proteasome 20S beta 6
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Proteasome 20S beta 6抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the peptidyl glutamyl-like activity. May catalyze basal processing of intracellular antigens.
Function:
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the peptidyl glutamyl-like activity. May catalyze basal processing of intracellular antigens.
Subunit:
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. This subunit can be displaced by the equivalent immune-specific subunit PSMB9. Interacts with HIV-1 TAT protein.
Subcellular Location:
Cytoplasm. Nucleus.
Similarity:
Belongs to the peptidase T1B family.
SWISS:
P28072
Gene ID:
5694
Proteasome 20S beta 6抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Proteasome 20S beta 6抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 phospho-Bcl-xL (Thr115) 磷酸化Bcl-xL蛋白抗体
合格 合格 phospho-Smad3 (Ser204) 磷酸化细胞信号转导分子SMAD3抗体
合格 phospho-CDKN1A/p21 (Thr145) 磷酸化p21蛋白抗体
合格 phospho-CDKN1A/p21 (Thr57) 磷酸化p21蛋白抗体
合格 phospho-CDKN1A/p21 (Ser146) 磷酸化p21蛋白抗体
合格 Phospho-Caspase-9 (Ser196) 磷酸化半胱氨酸蛋白酶9抗体
合格 phospho-CREB-1(Ser142) 磷酸化CREB-1抗体
合格 合格 phospho-CREB-1(Ser121) 磷酸化CREB-1抗体
合格 phospho-CDKN1A/p21 (Ser130) 磷酸化p21蛋白抗体
合格 phospho-CDKN1B (Thr198) 磷酸化P27抗体/周期素依赖激酶抑制剂抗体
合格 Cyclin E2 周期素E2抗体
合格 phospho-CASC3(Tyr181) 磷酸化肿瘤易感候选基因3抗体
合格 phospho-DAXX (Ser517) 磷酸化Fas相关死亡结构域蛋白抗体
合格 phospho-Desmin (Thr16) 磷酸化结蛋白抗体
合格 phospho-Desmin (Thr76 + Thr77) 磷酸化结蛋白抗体
合格 phospho-E2F1 (Ser337) 磷酸化转录因子E2F-1抗体
合格 phospho-EGFR (Tyr1110) 磷酸化表皮生长因子受体抗体
合格 phospho-EGFR (Ser1142) 磷酸化表皮生长因子受体抗体
合格 phospho-CD95/FAS (Tyr291) 磷酸化载脂蛋白A1抗体