PSMA4抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 12260R
英文名称PSMA4
中文名称蛋白酶体PSMα4/20S Proteasome α4抗体
别 名HC9; HsT17706; Macropain subunit C9; MGC111191; MGC12467; MGC24813; Multicatalytic endopeptidase complex subunit C9; Proteasome (prosome macropain) subunit alpha type 4; Proteasome alpha 4 subunit; Proteasome component C9; Proteasome subunit alpha type 4; Proteasome subunit alpha type-4; Proteasome subunit HC9; Proteasome subunit L; PSA4_HUMAN; PSC9; PSMA 4; psmA4.
说 明 书100ul 200ul
研究领域细胞生物 泛素
抗体来源Rabbit
克隆类型Polyclonal
PSMA4抗体交叉反应 Human, Mouse, Rat, Pig, Horse, Monkey,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量29kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from Human PSMA4
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
PSMA4抗体保存条件Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈.
PubMedPubMed
产品介绍background:
The proteasome represents a large protein complex that exists inside all eukaryotes and archaea, and in some bacteria. The main function of proteasomes is to degrade unnecessary or damaged proteins by proteolysis. The most common form of the proteasome, known as the 26S Proteasome, contains one 20S Proteasome core particle structure and two 19S regulatory caps. The 20S Proteasome core is hollow and forms an enclosed cavity, where proteins are degraded, as well as openings at the two ends to allow the target protein to enter. The 20S Proteasome core particle contains many subunits, depending on the organism. All of the subunits fall into one of two types: alpha subunits, which are structural, serve as docking domains for the regulatory particles and exterior gates blocking unregulated access to the interior cavity; or beta subunits, which are predominantly catalytic. The outer two rings in the proteasome consist of seven ?subunits each, and the inner two rings each consist of seven beta subunits.
Function:
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Subcellular Location:
Cytoplasm. Nucleus.
Similarity:
Belongs to the peptidase T1A family.
SWISS:
P25789
Gene ID:
5685
PSMA4抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
PSMA4抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 phospho-Tau protein (Ser396) 磷酸化微管相关蛋白抗体
合格 Phospho-HER3 (Tyr1289) 磷酸化HER3抗体
合格 Phospho-Insulin Receptor Beta (Tyr1355) 磷酸化胰岛素受体β抗体
合格 Phospho-Insulin Receptor Beta (Tyr1361) 磷酸化胰岛素受体β抗体
合格 合格 Phospho-mTOR (Ser2448) 磷酸化雷帕霉素靶蛋白抗体
合格 合格 Phospho-mTOR (Ser2481) 磷酸化雷帕霉素靶蛋白抗体
合格 合格 Phospho-p70 S6 Kinase Beta 2 (Tyr389) 磷酸化核糖体S6蛋白激酶β2抗体
合格 合格 Phospho-p70 S6 Kinase Beta (Thr228) 磷酸化核糖体S6蛋白激酶β2抗体
合格 Synapsin 1 神经突触素1抗体
合格 Mre11 DNA损伤关键蛋白Mre11抗体
合格 MST1 蛋白激酶MST抗体
合格 Nucleophosmin 核仁磷酸蛋白抗体
合格 合格 FCGR1A/CD64 **球蛋白G Fc段受体I
合格 Nur77 孤儿核受体蛋白Nur77抗体
合格 ID2 DNA结合抑制因子2抗体
合格 SLK 酪氨酸蛋白激酶Fyn/同步原癌基因抗体
合格 KMT6 抑癌蛋白EZH2抗体
合格 PFKFB3/PFK2 果糖-2,6-二磷酸酶3/磷酸果糖激酶2抗体
合格 ID1 DNA结合抑制因子1抗体
合格 HIRA 组蛋白替换精蛋白DGGR1抗体
合格 合格 PLCG 2 磷酯酶Cγ2抗体