PSMA7抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 9356R
英文名称PSMA7
中文名称蛋白酶体PSMα7抗体
别 名C6 antibody HSPC; Proteasome (prosome macropain) subunit alpha type 7; Proteasome alpha 7 subunit; Proteasome subunit alpha 4; Proteasome subunit alpha type 7; Proteasome subunit alpha type-7; Proteasome subunit RC6 1; Proteasome subunit RC6-1; Proteasome subunit XAPC7; PSA7_HUMAN; PSMA7; RC6 1; XAPC7.
说 明 书100ul 200ul
研究领域细胞生物 发育生物学 干细胞 细胞周期蛋白 细胞分化 细胞类型标志物
抗体来源Rabbit
克隆类型Polyclonal
PSMA7抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量28kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Proteasome 20S alpha 7
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis. Interacts with some important proteins involved in transcription factor regulation, cell cycle transition, viral replication and even tumor initiation and progression. Inhibits the transactivation function of HIF-1A under both normoxic and hypoxia-mimicking conditions. The interaction with EMAP2 increases the proteasome-mediated HIF-1A degradation under the hypoxic conditions. Plays a role in hepatitis C virus internal ribosome entry site-mediated translation. Mediates nuclear translocation of the androgen receptor (AR) and thereby enhances androgen-mediated transactivation. Promotes MAVS degradation and thereby negatively regulates MAVS-mediated innate immune response.
Function:
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis. Interacts with some important proteins involved in transcription factor regulation, cell cycle transition, viral replication and even tumor initiation and progression. Inhibits the transactivation function of HIF-1A under both normoxic and hypoxia-mimicking conditions. The interaction with EMAP2 increases the proteasome-mediated HIF-1A degradation under the hypoxic conditions. Plays a role in hepatitis C virus internal ribosome entry site-mediated translation. Mediates nuclear translocation of the androgen receptor (AR) and thereby enhances androgen-mediated transactivation. Promotes MAVS degradation and thereby negatively regulates MAVS-mediated innate immune response.
Subunit:
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. PSMA7 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly. Interacts with HIV-1 TAT protein. Interacts with hepatitis B virus X protein (HBX). Interacts with HIF1A. Interacts with RAB7A. Interacts with PARK2. Interacts with ABL1 and ABL2. Interacts with EMAP2. Interacts with MAVS.
Subcellular Location:
Cytoplasm. Nucleus.
Post-translational modifications:
Phosphorylation by ABL1 or ABL2 leads to an inhibition of proteasomal activity and cell cycle transition blocks.
Similarity:
Belongs to the peptidase T1A family.
Gene ID:
5688
PSMA7抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
PSMA7抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 Phospho-Tyk2(Tyr1054 + Tyr1055) 磷酸化非受体酪氨酸蛋白激酶2抗体
合格 合格 Phospho-TAK1(Thr187) 磷酸化转化生长因子β活化激酶1
合格 Phospho-Tuberin(Tyr1571) 磷酸化马铃薯球蛋白(结节性硬化)抗体
合格 Phospho-Tuberin (Thr927) 磷酸化马铃薯球蛋白(结节性硬化)抗体
合格 phospho-Tau (Ser396) 磷酸化微管相关蛋白抗体
合格 Phospho-eNOS (Ser1177) 磷酸化一氧化氮合成酶3（内皮型）抗体
合格 Phospho-Tie2 (Tyr992) 磷酸化血管生成素受体2抗体
合格 phospho-TOP2A (Ser1106) 磷酸化DNA拓普西异构酶Ⅱ抗体
合格 Phospho-MAP3K8 (Ser400) 磷酸化丝裂原活化蛋白激酶激酶8抗体
合格 Phospho-TrkA(Tyr496) +TrkB(Tyr516) 磷酸化酪氨酸激酶A抗体
合格 phospho-VEGF receptor 2 (Tyr1059) 磷酸化血管内皮生长因子受体2抗体
合格 phospho-VEGF receptor 2 (Tyr996) 磷酸化血管内皮生长因子受体2抗体
合格 合格 合格 Phospho-Vimentin (Ser56) 磷酸化波形蛋白抗体
合格 合格 合格 Phospho-Vimentin (Ser82) 磷酸化波形蛋白抗体
合格 Phospho-Caveolin-1 (Tyr14) 磷酸化细胞质膜微囊蛋白-1抗体
合格 Phospho-CDK2 (Thr160) 磷酸化周期素依赖性激酶2抗体
合格 Phospho-NFKB p65(Ser468) 磷酸化细胞核因子NF-κB p65抗体
合格 合格 phospho-Tau (Ser422) 磷酸化微管相关蛋白抗体