PSMB11抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 19462R
英文名称PSMB11
中文名称蛋白酶体β亚基11抗体
别 名BETA5T; Proteasome (prosome, macropain) subunit beta type 11; Proteasome subunit beta 5T; Proteasome subunit beta type-11; Proteasome subunit beta-5t; PSB11_HUMAN; PSMB11.
说 明 书100ul 200ul
研究领域细胞生物 **细胞 t-**细胞 泛素
抗体来源Rabbit
克隆类型Polyclonal
PSMB11抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Horse,
产品应用WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量33kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human PSMB11
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
PSMB11抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Proteasomes generate peptides that are presented by major histocompatibility complex (MHC) I molecules to other cells of the immune system. Proteolysis is conducted by 20S proteasomes, complexes of 28 subunits arranged as a cylinder in 4 heteroheptameric rings: alpha-1 to -7, beta-1 to -7, beta-1 to -7, and alpha-1 to -7. The catalytic subunits are beta-1 (PSMB6; MIM 600307), beta-2 (PSMB7; MIM 604030), and beta-5 (PSMB5; MIM 600306). Three additional subunits, beta-1i (PSMB9; MIM 177045), beta-2i (PSMB10; MIM 176847), and beta-5i (PSMB8; MIM 177046), are induced by gamma-interferon (IFNG; MIM 147570) and are preferentially incorporated into proteasomes to make immunoproteasomes. PSMB11, or beta-5t, is a catalytic subunit expressed exclusively in cortical thymic epithelial cells (Murata et al., 2007 [PubMed 17540904]).[supplied by OMIM, Mar 2008]
Function:
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Incorporated instead of PSMB5 or PSMB8, this unit reduces the chymotrypsin-like activity of the proteasome (By similarity). Plays a pivotal role in development of CD8-positive T cells.
Subunit:
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Incorporated instead of PSMB5 and PSMB8.
Subcellular Location:
Cytoplasm. Nucleus.
Similarity:
Belongs to the peptidase T1B family.
SWISS:
A5LHX3
Gene ID:
122706
PSMB11抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
PSMB11抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 Phospho-NMDAR1(Ser896) 磷酸化离子型谷氨酸受体1抗体
合格 NMDAR2B 谷氨酸受体2B抗体
合格 合格 Phospho-PAK4(Ser474) + PAK5(Ser602) + PAK6(Ser560) 磷酸化p21激活激酶4/5/6抗体
合格 Phospho-PDGFRA(Tyr849)/PDGFRB(Tyr857) 磷酸化血小板源性生长因子受体α/β抗体
合格 Phospho-PBK (Thr9) 磷酸化PDZ连接激酶/T-LAK细胞源蛋白激酶抗体
合格 合格 Phospho-PDK1(Ser241) 磷酸化3磷酸肌醇依赖性蛋白激酶1
合格 Phospho-PERK(Thr980) 磷酸化蛋白激酶样内质网激酶抗体
合格 合格 phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) 磷酸化磷脂酰肌醇激酶/PI3 Kinase P85α/γ抗体
合格 合格 Phospho-PKR (Thr451) 磷酸化蛋白激酶R抗体
合格 Phospho-PKR (Thr446 + Thr451) 磷酸化蛋白激酶R抗体
合格 合格 Phospho-PLCG 2 (Tyr1217) 磷酸化磷酯酶Cγ2抗体
合格 合格 Phospho-PLC beta3 (Ser1105) 磷酸化磷酯酶Cβ3抗体
合格 Phospho-PLC gamma 1(Ser1248) 磷酸化磷酯酶Cγ1抗体
合格 Phospho-PLC gamma 1 (Tyr783) 磷酸化磷酯酶Cγ1抗体
合格 Phospho-PLK1 (Ser137) 磷酸化丝/苏氨酸蛋白激酶Plk1抗体
合格 Phospho-PSD95 (Tyr236 + Tyr240) 磷酸化突触后密度蛋白95抗体
合格 合格 Phospho-PTEN(Ser380 + Thr382 + Thr383) 磷酸化肿瘤抑制基因PTEN抗体
合格 Phospho-Pin1 (Ser16) 磷酸化肽基脯氨酞顺反异构酶Pinl抗体
合格 Phospho-Progesterone Receptor(Ser190) 磷酸化孕**受体抗体
合格 Phospho-RPS6KA1 (Thr359 + Ser363) 磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体
合格 Phospho-RPS6KA1 (Thr573) 磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体