PSMC3抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 19463R
英文名称PSMC3
中文名称蛋白酶体26S亚基ATP酶3抗体
别 名26S protease regulatory subunit 6A; 26S proteasome AAA-ATPase subunit RPT5; Human immunodeficiency virus tat transactivator binding protein 1; MGC8487; Proteasome (prosome macropain) 26S subunit ATPase 3; Proteasome 26S ATPase subunit 3; Proteasome 26S subunit ATPase 3; Proteasome subunit P50; PRS6A_HUMAN; PSMC 3; PSMC3; Tat binding protein 1; TAT-binding protein 1; TBP-1; TBP1.
说 明 书100ul 200ul
研究领域细胞生物 结合蛋白 泛素
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Dog, Cow, Horse, Xenopus laevis
PSMC3抗体产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量49kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human PSMC3
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
PSMC3抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. [provided by RefSeq, Jul 2008]
Function:
The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). In case of HIV-1 infection, suppresses Tat-mediated transactivation.
Subcellular Location:
Cytoplasm. Nucleus.
Post-translational modifications:
Sumoylated by UBE2I in response to MEKK1-mediated stimuli.
Similarity:
Belongs to the AAA ATPase family.
SWISS:
P17980
Gene ID:
5702
PSMC3抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
PSMC3抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 Phospho-Histone H3 (Thr11) 磷酸化组蛋白H3抗体
合格 Phospho-Histone H3 (Thr3) 磷酸化组蛋白H3抗体
合格 合格 Phospho-IRAK1 (Thr209) 磷酸化白介素-1受体相关激酶1抗体
合格 Phospho-IRAK1 (Thr387) 磷酸化白介素-1受体相关激酶1抗体
合格 Phospho-IRF3 (Ser396) 磷酸化干扰素调节因子3
合格 合格 Phospho-IRF7 (Ser471 + Ser472) 磷酸化干扰素调节因子7抗体
合格 合格 phospho-IRS1 (Ser1101) 磷酸化胰岛素受体底物-1抗体
合格 phospho-IRS1 (Tyr612) 磷酸化胰岛素受体底物-1抗体
合格 phospho-IRS1 (Ser636 + Ser639) 磷酸化胰岛素受体底物-1抗体
合格 合格 phospho-IRS1 (Ser789) 磷酸化胰岛素受体底物-1抗体
合格 合格 phospho-c-Jun (Ser63) 磷酸化原癌基因c-Jun抗体
合格 Phospho-HER2 (Tyr1221 + Tyr1222) 磷酸化HER2受体抗体
合格 Phospho-HER3 (Tyr1197) 磷酸化HER3抗体
合格 Phospho-HSL (Ser863) 磷酸化***敏感脂肪酶抗体
合格 Phospho-HSP27 (Ser15) 磷酸化热休克蛋白27抗体
合格 phospho-IKK gamma (Ser85) 磷酸化KB抑制蛋白激酶γ抗体
合格 phospho-IKK gamma (Ser31) 磷酸化KB抑制蛋白激酶γ抗体
合格 Phospho-IKK alpha (Thr23) 磷酸化KB抑制蛋白激酶α/IKK α 抗体
合格 合格 Phospho-Jak1 (Tyr1034 + Tyr1035) 磷酸化蛋白质酪氨酸激酶JAK-1抗体