Rab27a抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 1581R
英文名称Rab27a
中文名称Rab27a蛋白抗体
别 名GS2; GTP-binding protein Ram; HsT18676; MGC117246; Rab-27; RAB-27A; RAB27; RAB27A; RAB27A member RAS oncogene family; RAM; Ras-related protein Rab-27A; Ras-related protein Rab27A; RB27A_HUMAN.
说 明 书100ul 200ul
研究领域肿瘤 细胞生物 转录调节因子 合成与降解 转运蛋白
抗体来源Rabbit
克隆类型Polyclonal
Rab27a抗体交叉反应 Human, Mouse, Rat, Pig, Cow, Horse, Sheep,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量25kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human RAM-11
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
Rab27a抗体产品介绍background:
Summary: The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq].
Function:
Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
Subunit:
Binds SYTL1, SLAC2B, MYRIP, SYTL3, SYTL4 and SYTL5 (By similarity). Binds MLPH and SYTL2. Interacts with UNC13D.
Subcellular Location:
Membrane; Lipid-anchor. Melanosome. Late endosome. Lysosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes to endosomal exocytic vesicles.
Tissue Specificity:
Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.
DISEASE:
Griscelli syndrome 2 (GS2) [MIM:607624]: Rare autosomal recessive disorder that results in pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes. GS2 patients also develop an uncontrolled T-lymphocyte and macrophage activation syndrome, known as hemophagocytic syndrome, leading to death in the absence of bone marrow transplantation. Neurological impairment is present in some patients, likely as a result of hemophagocytic syndrome. Note=The disease is caused by mutations affecting the gene represented in this entry.
Similarity:
Belongs to the small GTPase superfamily. Rab family.
Gene ID:
5873
Rab27a抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Rab27a抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 合格 PPP3CC 蛋白磷酸酶3调节亚基C抗体
合格 PPP3R2 蛋白磷酸酶3调节亚基R2抗体
合格 PPP1R16B 蛋白磷酸酶1调节亚基抑制蛋白16B抗体
合格 合格 PABP2 多聚腺苷酸结合蛋白2抗体
合格 PACSIN2 胞浆磷蛋白PACSIN2抗体
合格 合格 RUNX2 核心结合因子α1/成骨特异性转录因子/Cbfα1抗体
合格 Intestinal alkaline phosphatase 肠道碱性磷酸酶抗体
合格 合格 Placental alkaline phosphatase 胎盘碱性磷酸酶抗体
合格 CARD12 凋亡加强结构域蛋白12抗体
合格 PIM3 丝氨酸/苏氨酸蛋白激酶PIM3抗体
合格 phospho-GFAP (Thr7) 磷酸化胶质纤维酸性蛋白抗体
合格 C19orf56 19号染色体开放阅读框56抗体
合格 HSV1 + HSV2 gD 单纯疱疹病毒糖蛋白D抗体
合格 合格 UGT1A7 尿苷二磷酸葡糖醛基转移酶1A7抗体
合格 PCNA 增殖细胞核抗原抗体
合格 LY6G6F **细胞抗原6重复位点蛋白G6F抗体
合格 合格 合格 phospho-CDKN1B (Ser10) 磷酸化P27抗体/周期素依赖激酶抑制剂
合格 合格 Phospho-NFKB1 (Ser373) 磷酸化细胞核因子p50/k基因结合核因子抗体
合格 Tyrosine Hydroxylase Tyrosine Hydroxylase
合格 合格 PCNA 增殖细胞核抗原抗体
合格 Tyrosine Hydroxylase 酪氨酸羟化酶抗体
合格 合格 GPR106 G蛋白偶联受体106抗体
合格 EGF 表皮生长因子抗体
合格 ACTG1 肌动蛋白γ1抗体