RAB5抗体特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而,抗体可通过与病毒或**的特异性结合,直接发挥中和病毒的作用。
产品编号xy-6175R
英文名称RAB5
中文名称ras癌基因家族Rab5蛋白抗体
别 名RAB 5; RAB 5A; RAB5A; RAB5A member RAS oncogene family; RAB5A_HUMAN; RAS associated protein RAB5A; Ras related protein Rab 5A; Ras-related protein Rab-5A.
说 明 书50ul 100ul 200ul
研究领域细胞生物 神经生物学 信号转导 转运蛋白
抗体来源Rabbit
克隆类型Polyclonal
RAB5抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量24kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human RAB5.
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
RAB5抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Rab5-related subfamily. This subfamily includes Rab5 and Rab22 of mammals, Ypt51/Ypt52/Ypt53 of yeast, and RabF of plants. The members of this subfamily are involved in endocytosis and endocytic-sorting pathways. In mammals, Rab5 GTPases localize to early endosomes and regulate fusion of clathrin-coated vesicles to early endosomes and fusion between early endosomes. In yeast, Ypt51p family members similarly regulate membrane trafficking through prevacuolar compartments. GTPase activating proteins (GAPs) interact with GTP-bound Rab and accelerate the hydrolysis of GTP to GDP. Guanine nucleotide exchange factors (GEFs) interact with GDP-bound Rabs to promote the formation of the GTP-bound state. Rabs are further regulated by guanine nucleotide dissociation inhibitors (GDIs), which facilitate Rab recycling by masking C-terminal lipid binding and promoting cytosolic localization. Most Rab GTPases contain a lipid modification site at the C-terminus, with sequence motifs CC, CXC, or CCX. Lipid binding is essential for membrane attachment, a key feature of most Rab proteins. Due to the presence of truncated sequences in this CD, the lipid modification site is not available for annotation.
Subunit:
Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, RABEP1, SUN2, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3. Interacts with PIK3CB.
Subcellular Location:
Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome.
Similarity:
Belongs to the small GTPase superfamily. Rab family.
Gene ID:
5868
RAB5抗体(antibody, Ab)是由效应B细胞(效应**B细胞)分泌,机体用于抵御外来物质,如病毒,**等抗原,结构呈“Y”字型的球状蛋白质,仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质,均被称作抗原,因此对于抗抗体(能够结合抗体的抗体)来说,抗体本身也是一种抗原物质。
RAB5抗体普通抗体重链和轻链的结构
重链结构:普通的**球蛋白具有2条重链(H链),分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型,对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构: 普通**球蛋白具有2条轻链(L链),分子质量约25kDa,有κ链和λ链两种亚型,这两种轻链决定了Ig的亚型类别(IgG1,IgG2,IgG3,IgG4)。一个天然的Ig分子两条轻链总是相同的,但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同,正常人血清**球蛋白κ链:λ链约为2:1,而在小鼠的比例为20:1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应;经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段(fragment of antigen binding,Fab),相当于抗体分子的两个臂,由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段(fragment crystallizable,Fc)相当于Ig的CH2和CH3结构域,是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区,以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域,相当于Y字结构下面那一部分。
合格 OR1A1 嗅觉感受器蛋白1A1抗体
合格 SERCA3 肌浆/内质网钙ATP酶3抗体
合格 合格 SERGEF 鸟嘌呤核苷酸交换因子相关蛋白抗体
合格 SERINC2 丝氨酸组合因子2抗体
合格 合格 SerpinB8 丝氨酸蛋白酶抑制剂B8抗体
合格 合格 Thymidine kinase 2 胸苷激酶2抗体
合格 SETMAR 组蛋白赖氨酸N-甲基SETMAR抗体
合格 SF3B3 选择性剪接因子3B亚基3抗体
合格 SF3B4 选择性剪接因子3B亚基4抗体
合格 SFPQ 多聚嘧啶序列结合蛋白相关剪接因子PSF抗体
合格 SFRS14 富含精氨酸/丝氨酸剪接因子14抗体
合格 合格 PXK PXK蛋白抗体
合格 KLK14 激肽释放酶14抗体
合格 QSER1 富含谷氨酰胺和丝氨酸QSER1抗体
合格 QTRTD1 转运RNA核糖转移酶结构域蛋白QTRTD1抗体
合格 R3HDM2 含R3H结构域蛋白R3HDM2抗体
合格 RAB11-FIP2 Rab11家族相互作用蛋白2抗体
合格 RAB11FIP5 Rab11家族相互作用蛋白5抗体
合格 RAB30 ras基因相关蛋白Rab30抗体
合格 合格 LAMP1 溶酶体相关膜蛋白1抗体
合格 RAB31 ras基因相关蛋白Rab31抗体
合格 RAB39B ras基因相关蛋白RAB39B抗体
合格 RAB3IL1 RAB3A相关样蛋白1抗体
合格 RAD26L/C9orf102 9号染色体开放阅读框102抗体
合格 RAD51AP1 RAD51相互作用蛋白1抗体