Ras and Rab interactor 2抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 4332R
英文名称Ras and Rab interactor 2
中文名称RAS相关/干扰蛋白2抗体
别 名RIN2; MACS; RAB5 interacting protein 2; Ras and Rab interactor 2; RAS association (RalGDS/AF-6) domain containing protein JC265; Ras association domain family 4; Ras inhibitor JC265; Ras interaction/interference protein 2; RASSF4; RIN 2; RIN2_HUMAN.
说 明 书100ul 200ul
研究领域细胞生物 信号转导 G蛋白信号
抗体来源Rabbit
克隆类型Polyclonal
Ras and Rab interactor 2抗体交叉反应 Human, Mouse, Rat, Dog, Cow, Rabbit, Sheep,
产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量100kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Ras and Rab interactor 2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
Ras and Rab interactor 2抗体产品介绍background:
The RAB5 protein is a small GTPase involved in membrane trafficking in the early endocytic pathway. The protein encoded by this gene binds the GTP-bound form of the RAB5 protein preferentially over the GDP-bound form, and functions as a guanine nucleotide exchange factor for RAB5. The encoded protein is found primarily as a tetramer in the cytoplasm and does not bind other members of the RAB family. Mutations in this gene cause macrocephaly alopecia cutis laxa and scoliosis (MACS) syndrome, an elastic tissue disorder, as well as the related connective tissue disorder, RIN2 syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]
Function:
Ras effector protein. May function as an upstream activator and/or downstream effector for RAB5B in endocytic pathway. May function as a guanine nucleotide exchange (GEF) of RAB5B, required for activating the RAB5 proteins by exchanging bound GDP for free GTP.
Subunit:
Homotetramer; probably composed of anti-parallel linkage of two parallel dimers. Interacts with Ras. Interacts with RAB5B,with a much higher affinity for GTP-bound activated RAB5B. Does not interact with other members of the Rab family.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Widely expressed. Expressed in heart, kidney, lung placenta. Expressed at low level in skeletal muscle, spleen and peripheral blood.
DISEASE:
Defects in RIN2 are the cause of MACS syndrome (MACS) [MIM:613075]; also called macrocephaly alopecia cutis laxa and scoliosis syndrome. MACS is an autosomal-recessive inherited complex disorder of elastic tissue, characterized by sagging skin and occasionally by life-threatening visceral complications.
Similarity:
Belongs to the RIN (Ras interaction/interference) family.
Contains 1 Ras-associating domain.
Contains 1 SH2 domain.
Contains 1 VPS9 domain.
Gene ID:
54453
Ras and Rab interactor 2抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Ras and Rab interactor 2抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 MTHFD2L 亚甲基四氢叶酸脱氢酶2样蛋白抗体
合格 MTHFSD MTHFSD蛋白抗体
合格 MTMR12 肌微管素相关蛋白12抗体
合格 MTMR7 肌微管素相关蛋白7抗体
合格 MTMR9 肌微管素相关蛋白9抗体
合格 合格 CCR9 细胞表面趋化因子受体9抗体
合格 MTO1 MTO1蛋白抗体
合格 MTRF1 线粒体释放因子1样蛋白抗体
合格 合格 mtRNA polymerase 线粒体RNA聚合酶抗体
合格 合格 MTX1 MTX1蛋白抗体
合格 MTX2 MTX2蛋白抗体
合格 MTX3 MTX3蛋白抗体
合格 IL12 白介素12抗体
合格 合格 CRMP2/TOAD64 二氢嘧啶酶相关蛋白2抗体
合格 OR52R1 嗅觉受体52R1抗体
合格 OR5B12 嗅觉受体5AU12抗体
合格 OR5D14 嗅觉受体5D14抗体
合格 OR5H6 嗅觉受体5H6抗体
合格 合格 GLUR2 谷氨酸受体2抗体
合格 Orai1 嗅觉受体ai1抗体
合格 ORC4L ORC4L蛋白抗体
合格 合格 Orexin Receptor 1 丘脑分泌素受体1抗体
合格 GRM1 促代谢型谷氨酸受体1抗体
合格 HLADQA1 + HLADQA2 组织相容性抗原DQA1/DQA2抗体
合格 HMBOX1 HMBOX1蛋白抗体
合格 CD11a 整合素αL抗体