RASGRP2抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 19735R
英文名称RASGRP2
中文名称Ras蛋白特异鸟嘌呤核苷酸释放因子2抗体
别 名Calcium and DAG-regulated; Calcium and DAG-regulated guanine nucleotide exchange factor I; Calcium-and diacylglycerol-regulated guanine nucleotide exchange factor I; CalDAG-GEFI; Cdc25 like protein; Cdc25-like protein; F25B3.3 kinase like protein; F25B3.3 kinase-like protein; GRP2_HUMAN; hCDC25L; RAS guanyl releasing protein 2; RAS guanyl-releasing protein 2; Rasgrp2.
说 明 书100ul 200ul
研究领域细胞生物 信号转导 结合蛋白 G蛋白偶联受体 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
RASGRP2抗体交叉反应 Human, Mouse, Rat, Cow,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量69kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human RASGRP2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
RASGRP2抗体产品介绍background:
The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Function:
Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.
Subcellular Location:
Cytoplasm > cytosol. Cell membrane. Cell junction > synapse > synaptosome. Cell projection > ruffle membrane. Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons.
Tissue Specificity:
Detected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney.
Post-translational modifications:
Isoform 2 is palmitoylated and myristoylated while isoform 1.
Similarity:
Belongs to the RASGRP family.
Contains 2 EF-hand domains.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-GEF domain.
Gene ID:
10235
RASGRP2抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
RASGRP2抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 合格 Histone H1.X 组蛋白H1家族X抗体
合格 Dopamine D3 receptor 多巴胺受体D3抗体
合格 phospho-Histone H3 (Thr32) 磷酸化组蛋白H3抗体
合格 合格 phospho-TrkA (Tyr490) 磷酸化酪氨酸激酶A抗体
合格 Cytokeratin 7 细胞角蛋白7抗体
合格 HKDC1 己糖激酶结构域蛋白1抗体
合格 SHISA7 SHISA7蛋白抗体
合格 SHKBP1 SHKBP1蛋白抗体
合格 SHMT1 丝氨酸甲基化酶cSHMT抗体
合格 SHMT2 丝氨酸甲基化酶mSHMT抗体
合格 SHQ1 SHQ1蛋白抗体
合格 SIA8D 唾液酸转移酶8D抗体
合格 合格 合格 Dopamine D5 receptor 多巴胺受体D5抗体
合格 SIAH3 SIAH3蛋白抗体
合格 Sialidase 3 唾液酸酶3抗体
合格 DC-SIGNR1/CD209b CD209b抗体
合格 Desmoplakin I+II 桥粒斑蛋白1抗体
合格 SIL1 内质网分子伴侣SIL1抗体
合格 MAPKAP1/SIN1 丝裂原活化蛋白激酶相关蛋白1抗体
合格 合格 phospho-NFKB p65 (Ser281) 磷酸化细胞核因子抗体
合格 SIX1 同源盒蛋白SIX1抗体
合格 E2F6 转录因子E2F6抗体