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产品编号bs-0057R
英文名称Amylin
中文名称糖尿病相关肽/胰岛淀粉样肽抗体
别 名Islet amyloid polypeptide; IAPP; DAP; Diabetes associated peptide; IAP; Insulinoma amyloid peptide.
规格价格50ul/780元 购买 100ul/1280元 购买 200ul/1980元 购买 大包装/询价
说 明 书50ul 100ul 200ul
研究领域心血管 信号转导 糖尿病 新陈代谢
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Rabbit,
Amylin抗体产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
Amylin抗体产品介绍background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
Amylin抗体溶解方法：
1、在我们公司购买抗体后，查看标签上的规格，如：0.1ml、0.2ml
2、 以规格为0.1ml的冻干粉抗体为例（部分抗体为液体，无需再溶解）：
溶解方法一：用移液器加0.1ml双蒸水到试管内，混匀，-20℃保存；
若为其它规格（如：0.2ml）的冻干粉抗体，则加与标签上规格一致的（如：0.2ml）双蒸水溶解，混匀，-20℃保存。
溶解方法二：用移液器加 0.05ml（规格的 50%）双蒸水到试管内，再加 0.05ml（规格的 50%）
的甘油到试管内，混匀，-20℃保存； 若为其它规格（如：0.2ml）的冻干粉抗体，则加标签上规格的 50%（如：0.1ml）双蒸水，再加标签上规格的50%（如：0.1ml）甘油溶解，混匀，-20℃保存。
3、抗体的分装
抗体溶解后，将其分装成 5-10支（以0.1mlAmylin抗体为例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反复冻溶。
另：预试验可做几个梯度1：100、1：200、1：400稀释，如果背景高，还可再稀释，选一个*佳的稀释比例，再正式做，效果*好。工作液可用“抗体稀释液”稀释。
后续试验时，再使用对应的缓冲液稀释成工作液，即用即配。
我们的抗体（冻干粉），在常温放置下，至少一个月有效；若在-20℃下保存（推荐），至少三年有效。
Amylin抗体关联产品列表：
bs-0832R YES 合格 MICA MHC I类链相关蛋白A/组织相容性复合物MHCIa抗体
bs-0837R No 合格 Glutathione Reductase 谷胱甘肽还原酶抗体
bs-0842R No 合格 Myc tag c-Myc tag标签抗体
bs-0849R YES 合格 APOA1 载脂蛋白A1抗体
bs-0862R YES 合格 Insulin 胰岛素抗体
bs-0863R No 合格 GRO Alpha 黑色素瘤生长刺激活性蛋白α抗体 GROa
bs-0871R No 合格 合格 beta Amyloid 31-35 β淀粉样肽（31-35)/Aβ 31-35 抗体
bs-0872R YES 合格 beta-Amyloid(25-35) β淀粉样肽（25-35）抗体
bs-0876R YES 合格 合格 phospho-AKT (Ser473) 磷酸化蛋白激酶B抗体
bs-0877R YES 合格 合格 beta-Amyloid 1-40 (CT) β淀粉样肽1-40 C端/Aβ1-40 C端抗体
bs-0878R No 合格 CD144/VE Cadherin 上皮型钙粘附分子抗体
bs-0882R No 合格 Shiga-like toxin IIe variant subunit A 大肠杆菌志贺样**Ⅱ型突变体（O139菌型）抗体
bs-0889R No 合格 HSP90 alpha 热休克蛋白90α/HSP90 α抗体
bs-0894R No 合格 Phospho-Estrogen Receptor alpha (Ser167) 磷酸化雌**受体ER alpha 抗体
bs-0895R YES 合格 合格 FSH receptor 促卵泡刺**受体抗体
bs-0901R YES 合格 合格 LAMR1 层粘连蛋白受体1抗体
bs-0905R No 合格 p53 (Acetyl K382) 乙酰化P53(Lys382)抗体
bs-0908R YES 合格 合格 JAK2 蛋白质酪氨酸激酶JAK2抗体