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pDONR221/Kan

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产品名称: pDONR221/Kan
产品型号:
产品展商: XYbscience
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简单介绍

pDONR221/Kan菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。pDONR221/Kan收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。


pDONR221/Kan  的详细介绍

pDONR221/Kan

  • 产品信息
  • 基本信息
  • 质粒简介
  • 质粒图谱
  • 质粒序列

产品信息

产品货号 产品名称 产品规格 优惠价
XY0285 pDONR221/Kan

20μl

¥请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子: T7
复制子: ori
终止子: T1,T2
质粒分类: 植物系列,gateway载体
质粒大小: 4762
质粒标签: ccdB
原核抗性: Kan
克隆菌株: DB3.1
培养条件: 37℃,有氧 LB
表达宿主: 植物细胞
5'测序引物: M13F:TGTAAAACGACGGCCAGT
3'测序引物: M13R:CAGGAAACAGCTATGACC

质粒简介

    pDONR™ vectors are Gateway®-adapted vectors designed to generate attL-flanked entry clones containing your gene of interest following recombination with an attB expression clone or an attB PCR product. After creating an entry clone, your gene of interest may then be easily shuttled into a large selection of expression vectors using the Gateway® LR recombination reaction. Refer to the following table for a list of the available pDONR™ vectors.

质粒图谱

质粒序列

LOCUS       Exported                4762 bp ds-DNA     circular PAT 14-SEP-2016
DEFINITION  Sequence 3 from Patent WO2012087903.
ACCESSION   JA808046
VERSION     .
KEYWORDS    
SOURCE      synthetic construct
  ORGANISM  synthetic construct
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   ALLEN SM, BRUGIERE N, KUMRIA R, LUCK S, MOHANTY A, MULLEN J, RAJA R,
            SAKAI H, TINGEY SV, WILLIAMS RW.
  TITLE     DROUGHT TOLERANT PLANTS AND RELATED CONSTRUCTS AND METHODS INVOLVING
            GENES ENCODING MATE-EFFLUX POLYPEPTIDES
  JOURNAL   Patent: WO 2012087903-A2 3 28-JUN-2012; DU PONT [US]; PIONEER HI 
            BRED INT [US]; ALLEN STEPHEN M [US]; BRUGIERE NORBERT [US]; KUMRIA 
            RATNA [IN]; LUCK STANLEY [US]; MOHANTY AMITABH [IN]; MULLEN JEFFREY 
            [US]; RAJA RUPA [IN]; SAKAI HAJIME [US]; TINGEY SCOTT V [US]; 
            WILLIAMS ROBERT WAYNE [US]
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.2.1
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="synthetic construct"
                     /note="gateway donor vector pDONR221"
     terminator      268..295
                     /note="rrnB T2 terminator"
     terminator      387..473
                     /note="rrnB T1 terminator"
     primer_bind     537..553
                     /note="M13 fwd"
     protein_bind    570..801
                     /note="attP1"
     CDS             complement(1197..1502)
                     /codon_start=1
                     /note="ccdB"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     misc_feature    1763..1771
                     /note="-10"
     misc_feature    1786..1791
                     /note="-35"
     CDS             complement(1847..2506)
                     /codon_start=1
                     /note="CmR"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(2507..2609)
                     /note="cat promoter"
     protein_bind    complement(2754..2985)
                     /note="attP2"
     promoter        complement(3004..3022)
                     /note="T7 promoter"
     primer_bind     complement(3027..3043)
                     /note="M13 rev"
     CDS             3156..3965
                     /codon_start=1
                     /note="KanR"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      4112..4700
                     /direction=RIGHT
                     /note="ori"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
      721 aaaacagact acataatact gtaaaacaca acatatccag tcactatgaa tcaactactt
      781 agatggtatt agtgacctgt agtcgaccga cagccttcca aatgttcttc gggtgatgct
      841 gccaacttag tcgaccgaca gccttccaaa tgttcttctc aaacggaatc gtcgtatcca
      901 gcctactcgc tattgtcctc aatgccgtat taaatcataa aaagaaataa gaaaaagagg
      961 tgcgagcctc ttttttgtgt gacaaaataa aaacatctac ctattcatat acgctagtgt
     1021 catagtcctg aaaatcatct gcatcaagaa caatttcaca actcttatac ttttctctta
     1081 caagtcgttc ggcttcatct ggattttcag cctctatact tactaaacgt gataaagttt
     1141 ctgtaatttc tactgtatcg acctgcagac tggctgtgta taagggagcc tgacatttat
     1201 attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca
     1261 gccacttctt ccccgataac ggagaccggc acactggcca tatcggtggt catcatgcgc
     1321 cagctttcat ccccgatatg caccaccggg taaagttcac gggagacttt atctgacagc
     1381 agacgtgcac tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc
     1441 tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc
     1501 atttcaccag cccctgttct cgtcagcaaa agagccgttc atttcaataa accgggcgac
     1561 ctcagccatc ccttcctgat tttccgcttt ccagcgttcg gcacgcagac gacgggcttc
     1621 attctgcatg gttgtgctta ccagaccgga gatattgaca tcatatatgc cttgagcaac
     1681 tgatagctgt cgctgtcaac tgtcactgta atacgctgct tcatagcata cctctttttg
     1741 acatacttcg ggtatacata tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat
     1801 acgcatactg ttatctggct tttagtaagc cggatccacg cggcgtttac gccccgccct
     1861 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     1921 agacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     1981 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     2041 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     2101 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     2161 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     2221 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     2281 ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     2341 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     2401 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     2461 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     2521 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     2581 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg
     2641 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca
     2701 caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttagtc gactacaggt
     2761 cactaatacc atctaagtag ttgattcata gtgactggat atgttgtgtt ttacagtatt
     2821 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     2881 tttctcgttc agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
     2941 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     3001 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     3061 ctcaaaatct ctgatgttac attgcacaag ataaaataat atcatcatga acaataaaac
     3121 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt
     3181 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
     3241 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag
     3301 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     3361 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
     3421 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc caggtattag
     3481 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     3541 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc
     3601 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
     3661 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg
     3721 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     3781 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     3841 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     3901 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
     3961 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg
     4021 acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     4081 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4141 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4201 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4261 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4321 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4381 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4441 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4501 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4561 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4621 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4681 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4741 gctggccttt tgctcacatg tt
//
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