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pCXUN-Myc

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产品名称: pCXUN-Myc
产品型号:
产品展商: XYbscience
产品文档: 无相关文档

简单介绍

pCXUN-Myc菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。pCXUN-Myc收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。


pCXUN-Myc  的详细介绍

pCXUN-Myc

  • 产品信息
  • 基本信息
  • 质粒简介
  • 质粒图谱
  • 质粒序列

产品信息

产品货号 产品名称 产品规格 优惠价
XY1621 pCXUN-Myc

20μl

¥请加QQ询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子: Ubi promoter
终止子: NOS terminator
质粒大小: 12041bp
质粒标签: N-Myc
原核抗性: 卡那霉素Kan
筛选标记: 潮霉素Hyg
克隆菌株: 大肠杆菌DB3.1
培养条件: 37℃,有氧,LB

质粒简介

        pCXUN-Myc质粒是一个植物过表达质粒,UBi启动子驱动Myc标签和目的基因融合表达。空载体用XcmI酶切后,会变成T载体,可连入带A的PCR产物,其携带的ccdb致死基因可显著提高基因克隆的阳性率。

质粒图谱




质粒序列

LOCUS       Exported               12041 bp ds-DNA     circular SYN 30-OCT-2017
DEFINITION  synthetic circular DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12041)
  TITLE     Direct Submission
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..12041
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      115..367
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             728..1033
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(1089..1118)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(1134..3124)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_feature    3365..3389
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4688..5317
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5751..6818
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6884..7078
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7422..7562
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7748..8336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8423..9217)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9642..9666
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9744..9918
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9958..10983)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(11050..11727)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 gaattcgagc tcggtacccc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
       61 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcccgatcta
      121 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
      181 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
      241 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
      301 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
      361 aacgatcggg gaaattcgga tccccaatac ttgtatgggg cttactaaaa gccagataac
      421 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
      481 gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga
      541 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
      601 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
      661 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc
      721 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
      781 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
      841 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
      901 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
      961 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
     1021 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gacccataca
     1081 agtattggaa gatcttcttc agaaatcaac ttttgttcca tggatccccc gggctgcaga
     1141 agtaacacca aacaacaggg tgagcatcga caaaagaaac agtaccaagc aaataaatag
     1201 cgtatgaagg cagggctaaa aaaatccaca tatagctgct gcatatgcca tcatccaagt
     1261 atatcaagat caaaataatt ataaaacata cttgtttatt ataatagata ggtactcaag
     1321 gttagagcat atgaatagat gctgcatatg ccatcatgta tatgcatcag taaaacccac
     1381 atcaacatgt atacctatcc tagatcgata tttccatcca tcttaaactc gtaactatga
     1441 agatgtatga cacacacata cagttccaaa attaataaat acaccaggta gtttgaaaca
     1501 gtattctact ccgatctaga acgaatgaac gaccgcccaa ccacaccaca tcatcacaac
     1561 caagcgaaca aaaagcatct ctgtatatgc atcagtaaaa cccgcatcaa catgtatacc
     1621 tatcctagat cgatatttcc atccatcatc ttcaattcgt aactatgaat atgtatggca
     1681 cacacataca gatccaaaat taataaatcc accaggtagt ttgaaacaga attctactcc
     1741 gatctagaac gaccgcccaa ccagaccaca tcatcacaac caagacaaaa aaaagcatga
     1801 aaagatgacc cgacaaacaa gtgcacggca tatattgaaa taaaggaaaa gggcaaacca
     1861 aaccctatgc aacgaaacaa aaaaaatcat gaaatcgatc ccgtctgcgg aacggctaga
     1921 gccatcccag gattccccaa agagaaacac tggcaagtta gcaatcagaa cgtgtctgac
     1981 gtacaggtcg catccgtgta cgaacgctag cagcacggat ctaacacaaa cacggatcta
     2041 acacaaacat gaacagaagt agaactaccg ggccctaacc atggaccgga acgccgatct
     2101 agagaaggta gagagggggg gggggggagg acgagcggcg taccttgaag cggaggtgcc
     2161 gacgggtgga tttgggggag atctggttgt gtgtgtgtgc gctccgaaca acacgaggtt
     2221 ggggaaagag ggtgtggagg gggtgtctat ttattacggc gggcgaggaa gggaaagcga
     2281 aggagcggtg ggaaaggaat cccccgtagc tgccgtgccg tgagaggagg aggaggccgc
     2341 ctgccgtgcc ggctcacgtc tgccgctccg ccacgcattt ctggatgccg acagcggagc
     2401 aagtccaacg gtggagcgga actctcgaga ggggtccaga ggcagcgaca gagatgccgt
     2461 gccgtctgct tcgcttggcc cgacgcgacg ctgctggttc gctggttggt gtccgttaga
     2521 ctcgtcgacg gcgtttaaca ggctggcatt atctactcga aacaagaaaa atgtttcctt
     2581 agttttttta atttcttaaa gggtatttgt ttaattttta gtcactttat tttattctat
     2641 tttatatcta aattattaaa taaaaaaact aaaatagagt tttagttttc ttaatttaga
     2701 ggctaaaata gaataaaata gatgtactaa aaaaattagt ctataaaaac cattaaccct
     2761 aaaccctaaa tggatgtact aataaaatgg atgaagtatt atataggtga agctatttgc
     2821 aaaaaaaaag gagaacacat gcacactaaa aagataaaac tgtagagtcc tgttgtcaaa
     2881 atactcaatt gtcctttaga ccatgtctaa ctgttcattt atatgattct ctaaaacact
     2941 gatattattg tagtactata gattatatta ttcgtagagt aaagtttaaa tatatgtata
     3001 aagatagata aactgcactt caaacaagtg tgacaaaaaa aatatgtggt aattttttat
     3061 aacttagaca tgcaatgctc attatctcta gagaggggca cgaccgggtc acgctgcact
     3121 gcaggaattc gatatcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     3181 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     3241 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     3301 gctagagcag cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag
     3361 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg
     3421 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     3481 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     3541 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt
     3601 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     3661 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     3721 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     3781 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     3841 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta gccctggcga cgttgtgaca
     3901 gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc
     3961 atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg
     4021 ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta
     4081 atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc
     4141 ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa
     4201 ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc
     4261 gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt
     4321 gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa
     4381 caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg
     4441 aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc
     4501 ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct
     4561 tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca
     4621 gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg
     4681 ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat
     4741 cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc
     4801 cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt
     4861 tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt
     4921 cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc
     4981 cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcaa ccgccgacct
     5041 ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt
     5101 cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg
     5161 gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc
     5221 cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca
     5281 ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg
     5341 agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca
     5401 acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg
     5461 gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg
     5521 ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg
     5581 aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt
     5641 ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg
     5701 ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgac ggtcgcaaac
     5761 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
     5821 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
     5881 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt
     5941 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
     6001 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
     6061 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
     6121 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
     6181 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
     6241 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
     6301 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
     6361 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
     6421 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
     6481 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
     6541 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
     6601 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
     6661 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
     6721 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
     6781 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
     6841 aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca
     6901 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
     6961 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
     7021 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
     7081 tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct
     7141 ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg
     7201 gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc
     7261 ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     7321 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     7381 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
     7441 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
     7501 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     7561 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7621 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7681 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7741 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7801 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7861 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7921 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7981 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8041 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8101 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8161 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8221 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8281 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8341 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8401 ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa tattttattt
     8461 tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc
     8521 cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc
     8581 cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt
     8641 ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat
     8701 catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat
     8761 cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg
     8821 tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct
     8881 cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg
     8941 cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa
     9001 caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca tcataggttg
     9061 tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct
     9121 tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca
     9181 gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct
     9241 acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt
     9301 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
     9361 ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac
     9421 gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc
     9481 ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt
     9541 acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat
     9601 tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt
     9661 gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga
     9721 attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg ttttaggaat
     9781 tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg gtttcttata
     9841 tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg gaactactca
     9901 cacattatta tggagaaact cgagcttgtc gatcgacaga tccggtcggc atctactcta
     9961 tttctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac
    10021 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc
    10081 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt
    10141 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag
    10201 tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat
    10261 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa
    10321 catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt
    10381 ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat
    10441 cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca
    10501 gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc
    10561 gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat
    10621 cgcatccata gcctccgcga ccggttgtag aacagcgggc agttcggttt caggcaggtc
    10681 ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctaaactc
    10741 cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata
    10801 acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc
    10861 tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac
    10921 gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt
    10981 catatctcat tgccccccgg atctgcgaaa gctcgagaga gatagatttg tagagagaga
    11041 ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt ccttatatag aggaaggtct
    11101 tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc
    11161 acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg
    11221 ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg
    11281 caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag
    11341 atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca
    11401 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
    11461 tgctccacca tgttatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct
    11521 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca
    11581 tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt
    11641 tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc
    11701 gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg
    11761 atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggcaagctg ctctagccaa
    11821 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    11881 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    11941 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    12001 gataacaatt tcacacagga aacagctatg accatgatta c
//
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