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pCX-DG

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产品名称: pCX-DG
产品型号:
产品展商: XYbscience
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简单介绍

pCX-DG质粒是一个植物过表达质粒,35S启动子驱动GFP绿色荧光蛋白和目的基因融合表达,pCX-DG在ccdb致死基因的作用下,克隆基因变得更加容易,阳性率高。


pCX-DG  的详细介绍

pCX-DG

  • 产品信息
  • 基本信息
  • 质粒简介
  • 质粒图谱
  • 质粒序列

产品信息

产品货号 产品名称 产品规格 优惠价
XY1625 pCX-DG

20μl

¥请加QQ询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子: CaMV35S promoter
终止子: NOS terminator
质粒大小: 11277bp
原核抗性: 卡那霉素Kan
筛选标记: 潮霉素Hyg
克隆菌株: 大肠杆菌DB3.1
培养条件: 37℃,有氧,LB

质粒简介

             pCX-DG质粒是一个植物过表达质粒,35S启动子驱动GFP绿色荧光蛋白和目的基因融合表达,在ccdb致死基因的作用下,克隆基因变得更加容易,阳性率高。

质粒图谱


质粒序列

LOCUS       Exported               11277 bp ds-DNA     circular SYN 30-OCT-2017
DEFINITION  synthetic circular DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11277)
  TITLE     Direct Submission
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..11277
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    196..655
                     /label=CaMV35S promoter
     regulatory      673..682
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             679..1392
                     /codon_start=1
                     /label=GFP
                     /translation="MGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTMGYGVLCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    1393..1458
                     /label=MCS
                     /note="multiple cloning site"
     CDS             1803..2108
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     terminator      2327..2579
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2601..2625
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3924..4553
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4987..6054
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6120..6314
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6658..6798
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6984..7572)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7659..8453)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8878..8902
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8980..9154
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9194..10219)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10286..10963)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 gaattcgagc tcggtaccca ctggattttg gttttaggaa ttagaaattt tattgataga
       61 agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa
      121 accctatagg aaccctaatt cccttatctg ggaactactc acacattatt atggagaaac
      181 tcgaaattcg agctccaccg cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc
      241 caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt
      301 ctccgctcat gatccgggga attaattccg atcctatctg tcacttcatc aaaaggacag
      361 tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcattc
      421 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
      481 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
      541 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
      601 gttcatttca tttggagagg acacgctgaa atcaccagtc tctctctaca agatcctcta
      661 gcgctaccgg tcgccaccat gggtaaagga gaagaacttt tcactggagt tgtcccaatt
      721 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      781 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      841 gttccatggc caacacttgt cactactatg ggttatggtg ttctatgctt ttcaagatac
      901 ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      961 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
     1021 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
     1081 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
     1141 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
     1201 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     1261 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
     1321 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
     1381 gaactataca agtccggact cagatctcga gctcaagctt cgaattctgc agtcgacggt
     1441 accgcgggcc cgggatcccc aatacttgta tggggcttac taaaagccag ataacagtat
     1501 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     1561 atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct
     1621 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     1681 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     1741 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg
     1801 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     1861 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     1921 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     1981 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     2041 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     2101 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgaccc atacaagtat
     2161 tggggatcca ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt
     2221 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     2281 attgttgttg ttgtgtgaat tacaggtgac cagctcgaat ttccccgatc gttcaaacat
     2341 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     2401 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     2461 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     2521 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     2581 ggaattaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
     2641 gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
     2701 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
     2761 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
     2821 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
     2881 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
     2941 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
     3001 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
     3061 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctagccc
     3121 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
     3181 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
     3241 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
     3301 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
     3361 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
     3421 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
     3481 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
     3541 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
     3601 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
     3661 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
     3721 cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
     3781 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
     3841 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat
     3901 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
     3961 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg
     4021 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
     4081 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
     4141 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
     4201 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
     4261 gggcaaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
     4321 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
     4381 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
     4441 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
     4501 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
     4561 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
     4621 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
     4681 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
     4741 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg
     4801 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
     4861 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
     4921 gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
     4981 cgtgacggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
     5041 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
     5101 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
     5161 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
     5221 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
     5281 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
     5341 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
     5401 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
     5461 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
     5521 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
     5581 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
     5641 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
     5701 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
     5761 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
     5821 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
     5881 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
     5941 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
     6001 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
     6061 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct
     6121 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
     6181 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
     6241 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
     6301 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
     6361 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
     6421 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
     6481 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
     6541 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     6601 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     6661 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
     6721 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     6781 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6841 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6901 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6961 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7021 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7081 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7141 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7201 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7261 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7321 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7381 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     7441 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7501 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7561 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7621 aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa
     7681 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
     7741 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
     7801 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
     7861 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
     7921 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
     7981 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
     8041 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
     8101 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
     8161 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
     8221 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
     8281 tccacatcat aggttgtccc tttataccgg ctgtccgtca tttttaaata taggttttca
     8341 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
     8401 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
     8461 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
     8521 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
     8581 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt
     8641 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
     8701 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
     8761 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
     8821 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
     8881 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac
     8941 gtttttaatg tactgaatta acgccgaatt aattcggggg atctggattt tagtactgga
     9001 ttttggtttt aggaattaga aattttattg atagaagtat tttacaaata caaatacata
     9061 ctaagggttt cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt
     9121 atctgggaac tactcacaca ttattatgga gaaactcgag cttgtcgatc gacagatccg
     9181 gtcggcatct actctatttc tttgccctcg gacgagtgct ggggcgtcgg tttccactat
     9241 cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg
     9301 tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag
     9361 ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga
     9421 gcatatacgc ccggagtcgt ggcgatcctg caagctccgg atgcctccgc tcgaagtagc
     9481 gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt
     9541 attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt
     9601 ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt
     9661 cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt
     9721 ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc
     9781 atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa
     9841 gatcggccgc agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca gcgggcagtt
     9901 cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca
     9961 ggctctcgct aaactcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa
    10021 agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga
    10081 catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct
    10141 gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg
    10201 tgagttcagg ctttttcata tctcattgcc ccccggatct gcgaaagctc gagagagata
    10261 gatttgtaga gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt
    10321 atatagagga aggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga
    10381 gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
    10441 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat
    10501 agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct
    10561 tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct
    10621 ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga
    10681 gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct ttgaagacgt
    10741 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
    10801 actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta
    10861 ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa
    10921 tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
    10981 tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc
    11041 aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    11101 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
    11161 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
    11221 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattac
//
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