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pCAMBIA3300

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产品名称: pCAMBIA3300
产品型号:
产品展商: XYbscience
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简单介绍

pCAMBIA3300菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。pCAMBIA3300收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。


pCAMBIA3300  的详细介绍

pCAMBIA3300

  • 产品信息
  • 基本信息
  • 质粒简介
  • 质粒序列

产品信息

产品货号 产品名称 产品规格 优惠价
XY0289 pCAMBIA3300

20μl

¥请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子: Lac,CaMV 35S
复制子: ori
质粒分类: 植物系列,蛋白过表达载体
质粒大小: 8428bp
原核抗性: Kan
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 植物细胞
5'测序引物: 根据序列设计引物
3'测序引物: 根据序列设计引物

质粒图谱


质粒序列

LOCUS       Exported                8428 bp ds-DNA     circular SYN 14-SEP-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCAMBIA3300
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.2.1
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..8428
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(38..715)
                     /note="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    906..927
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        942..972
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    980..996
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1004..1020
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1016..1249
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /note="lacZ-alpha"
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     misc_feature    1030..1086
                     /note="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1090..1106)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1309..1333
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2633..3262
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /note="pVS1 StaA"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3696..4763
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /note="pVS1 RepA"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     misc_feature    5367..5507
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5693..6281)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6368..7162)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7587..7611
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    7689..7863
                     /note="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7870..8421)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /note="BlpR"
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
ORIGIN
        1 tcgagagaga tagatttgta gagagagact ggtgatttca gcgtgtcctc tccaaatgaa
       61 atgaacttcc ttatatagag gaaggtcttg cgaaggatag tgggattgtg cgtcatccct
      121 tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
      181 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
      241 atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt
      301 ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
      361 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
      421 gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttatcacatc aatccacttg
      481 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
      541 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
      601 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
      661 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
      721 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
      781 caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc gcgttggccg
      841 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
      901 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
      961 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     1021 catgattacg aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc
     1081 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     1141 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     1201 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag
     1261 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata
     1321 ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg
     1381 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
     1441 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca
     1501 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc
     1561 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact
     1621 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
     1681 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg
     1741 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag
     1801 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac
     1861 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc
     1921 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
     1981 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc
     2041 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc
     2101 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa
     2161 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc
     2221 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc
     2281 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc
     2341 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg
     2401 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc
     2461 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa
     2521 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
     2581 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
     2641 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
     2701 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
     2761 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
     2821 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
     2881 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
     2941 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
     3001 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
     3061 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
     3121 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
     3181 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
     3241 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
     3301 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
     3361 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
     3421 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
     3481 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
     3541 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
     3601 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg
     3661 cactggaacc cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac
     3721 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
     3781 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
     3841 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
     3901 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
     3961 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
     4021 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
     4081 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
     4141 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
     4201 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
     4261 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
     4321 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
     4381 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
     4441 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
     4501 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
     4561 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
     4621 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
     4681 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
     4741 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
     4801 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc
     4861 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
     4921 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
     4981 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
     5041 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
     5101 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca
     5161 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag
     5221 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     5281 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     5341 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     5401 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     5461 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     5521 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5581 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     5641 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5701 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5761 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5821 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5881 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5941 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6001 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6061 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6121 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6181 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     6241 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6301 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt
     6361 ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct
     6421 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga
     6481 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat
     6541 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt
     6601 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg
     6661 gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat
     6721 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg
     6781 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag
     6841 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca
     6901 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt
     6961 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc
     7021 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag
     7081 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg
     7141 gtgatattct cattttagcc atttattatt tccttcctct tttctacagt atttaaagat
     7201 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa
     7261 ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt
     7321 atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta
     7381 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc
     7441 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg
     7501 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
     7561 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg
     7621 cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta
     7681 attcggggga tctggatttt agtactggat tttggtttta ggaattagaa attttattga
     7741 tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag
     7801 cgaaacccta taggaaccct aattccctta tctgggaact actcacacat tattatggag
     7861 aaactcgagt caaatctcgg tgacgggcag gaccggacgg ggcggtaccg gcaggctgaa
     7921 gtccagctgc cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag
     7981 catgccgcgg ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag
     8041 cccgatgaca gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt
     8101 gtagagcgtg gagcccagtc ccgtccgctg gtggcggggg gagacgtaca cggtcgactc
     8161 ggccgtccag tcgtaggcgt tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac
     8221 ctcgccgtcc acctcggcga cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc
     8281 cgtccactcc tgcggttcct gcggctcggt acggaagttg accgtgcttg tctcgatgta
     8341 gtggttgacg atggtgcaga ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc
     8401 cgggcgtcgt tctgggctca tggtagac
//
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