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pCAMBIA1304

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产品名称: pCAMBIA1304
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产品展商: XYbscience
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简单介绍

pCAMBIA1304菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。pCAMBIA1304收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。


pCAMBIA1304  的详细介绍

pCAMBIA1304

  • 产品信息
  • 基本信息
  • 质粒简介
  • 质粒图谱
  • 质粒序列

产品信息

产品货号 产品名称 产品规格 优惠价
XY0279 pCAMBIA1304

20μl

¥请加QQ询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子: CaMV 35S promoter
复制子: pVS1 oriV,ori
终止子: NOS
质粒大小: 12361bp
原核抗性: Kan
筛选标记: HygR
克隆菌株: DH5α
培养条件: 37℃,有氧,LB
表达宿主: 植物
5'测序引物: M13F:TGTAAAACGACGGCCAGT

质粒简介

     Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and the mgfp5:gusA fusion reporter genes.The pCambia vector backbone is derived from the pPZP vectors. The pCambia1304 vector offers:1.high copy number in E.coli for high DNA yield 2.pVS1 replicon for high stability in Agrobacterium 3.small size 4.restriction sites for modular plasmid modifications and small but adequate polylinkers for introducing DNA of interest 5.bacterial selection with kanamycin 6.plant selection with hygromycin B 7.simple means to construct translational fusions to mgfp5-gusA reporter genes.

质粒图谱


质粒序列

LOCUS       Exported               12361 bp ds-DNA     circular SYN 14-SEP-2016
DEFINITION  synthetic circular DNA
KEYWORDS    Untitled 4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12361)
  TITLE     Direct Submission
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..12361
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             17..727
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /note="mgfp5"
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             734..2539
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /note="GUS"
                     /translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA
                     VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM
                     EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF
                     FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV
                     VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQFL
                     INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA
                     DEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
                     NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             2546..2563
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     terminator      2595..2847
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2869..2893
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4193..4822
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /note="pVS1 StaA"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5256..6323
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /note="pVS1 RepA"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6389..6583
                     /note="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6927..7067
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7253..7841)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7928..8722)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9147..9171
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9249..9423
                     /note="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9463..10488)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10556..11233)
                     /note="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    11424..11445
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11460..11490
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    11498..11514
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11522..11538
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             11534..11767
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /note="lacZ-alpha"
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     misc_feature    11548..11604
                     /note="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(11608..11624)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        12001..12346
                     /note="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 catggtagat ctgactagta aaggagaaga acttttcact ggagttgtcc caattcttgt
       61 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      121 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      181 gtggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttcaa gatacccaga
      241 tcatatgaag cggcacgact tcttcaagag cgccatgcct gagggatacg tgcaggagag
      301 gaccatcttc ttcaaggacg acgggaacta caagacacgt gctgaagtca agtttgaggg
      361 agacaccctc gtcaacagga tcgagcttaa gggaatcgat ttcaaggagg acggaaacat
      421 cctcggccac aagttggaat acaactacaa ctcccacaac gtatacatca tggccgacaa
      481 gcaaaagaac ggcatcaaag ccaacttcaa gacccgccac aacatcgaag acggcggcgt
      541 gcaactcgct gatcattatc aacaaaatac tccaattggc gatggccctg tccttttacc
      601 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
      661 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
      721 atacaaagct agtttacgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg
      781 cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag
      841 cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga
      901 tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa
      961 aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt
     1021 gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga
     1081 tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga
     1141 actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa
     1201 gcagtcttac ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta
     1261 caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg
     1321 taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg
     1381 tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt
     1441 gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa
     1501 aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa
     1561 gggccaacag ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga
     1621 agatgcggac ttacgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt
     1681 aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat
     1741 gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt
     1801 tcagctgtct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga
     1861 agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc
     1921 gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg
     1981 tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc
     2041 gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag
     2101 cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc aaagcggcga
     2161 tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg agaaactgca
     2221 tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta
     2281 caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt
     2341 tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc
     2401 gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc
     2461 gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc
     2521 gcagcaggga ggcaaacaag ctagccacca ccaccaccac cacgtgtgaa ttggtgacca
     2581 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     2641 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     2701 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     2761 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     2821 gcgcggtgtc atctatgtta ctagatcggg aattaaacta tcagtgtttg acaggatata
     2881 ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg
     2941 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
     3001 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca
     3061 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc
     3121 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact
     3181 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
     3241 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg
     3301 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag
     3361 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac
     3421 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc
     3481 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
     3541 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc
     3601 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc
     3661 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa
     3721 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc
     3781 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc
     3841 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc
     3901 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg
     3961 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc
     4021 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa
     4081 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
     4141 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
     4201 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
     4261 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
     4321 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
     4381 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
     4441 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
     4501 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
     4561 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
     4621 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
     4681 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
     4741 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
     4801 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
     4861 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
     4921 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
     4981 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
     5041 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
     5101 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
     5161 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg
     5221 cactggaacc cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac
     5281 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
     5341 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
     5401 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
     5461 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
     5521 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
     5581 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
     5641 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
     5701 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
     5761 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
     5821 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
     5881 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
     5941 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
     6001 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
     6061 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
     6121 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
     6181 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
     6241 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
     6301 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
     6361 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc
     6421 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
     6481 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
     6541 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
     6601 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
     6661 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca
     6721 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag
     6781 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     6841 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     6901 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     6961 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     7021 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     7081 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7141 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7201 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7261 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7321 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7381 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7441 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7501 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7561 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7621 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7681 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7741 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7801 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7861 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt
     7921 ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct
     7981 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga
     8041 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat
     8101 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt
     8161 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg
     8221 gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat
     8281 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg
     8341 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag
     8401 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca
     8461 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt
     8521 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc
     8581 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag
     8641 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg
     8701 gtgatattct cattttagcc atttattatt tccttcctct tttctacagt atttaaagat
     8761 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa
     8821 ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt
     8881 atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta
     8941 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc
     9001 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg
     9061 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
     9121 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg
     9181 cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta
     9241 attcggggga tctggatttt agtactggat tttggtttta ggaattagaa attttattga
     9301 tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag
     9361 cgaaacccta taggaaccct aattccctta tctgggaact actcacacat tattatggag
     9421 aaactcgagc ttgtcgatcg acagatccgg tcggcatcta ctctatttct ttgccctcgg
     9481 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca
     9541 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac
     9601 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct
     9661 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagtcgtg gcgatcctgc
     9721 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg
     9781 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
     9841 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
     9901 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc
     9961 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg
    10021 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
    10081 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatagcctc
    10141 cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
    10201 ctgtgcacgg cgggagatgc aataggtcag gctctcgcta aactccccaa tgtcaagcac
    10261 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa
    10321 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
    10381 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc
    10441 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc
    10501 cccgggatct gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc
    10561 gtgtcctctc caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg
    10621 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga
    10681 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
    10741 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
    10801 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
    10861 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
    10921 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
    10981 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct
    11041 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc
    11101 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt
    11161 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg
    11221 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta
    11281 gacgagagtg tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct
    11341 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
    11401 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    11461 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    11521 acaggaaaca gctatgacca tgattacgaa ttcgagctcg gtacccgggg atcctctaga
    11581 gtcgacctgc aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
    11641 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
    11701 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
    11761 atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttagcttcat
    11821 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
    11881 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
    11941 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
    12001 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
    12061 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
    12121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
    12181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
    12241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
    12301 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactcttga
    12361 c
//
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