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pRI101-AN双子叶植物发根农杆菌质粒

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产品名称: pRI101-AN双子叶植物发根农杆菌质粒
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产品展商: XYbscience
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简单介绍

pRI101-AN双子叶植物发根农杆菌质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。pRI101-AN双子叶植物发根农杆菌质粒收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。


pRI101-AN双子叶植物发根农杆菌质粒  的详细介绍

pRI101-AN双子叶植物发根农杆菌质粒

  • 产品信息
  • 基本信息
  • 质粒简介
  • 质粒图谱
  • 质粒序列

产品信息

产品货号 产品名称 产品规格 优惠价
XY1785 pRI101-AN双子叶植物发根农杆菌质粒

1μg

¥请加QQ询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子: 35S
原核抗性: 卡那霉素Kan
筛选标记: 遗传霉素G418
克隆菌株: 大肠杆菌HB101
培养条件: 37℃

质粒简介

        pRI101-AN载体是一种在双子叶植物细胞中表达外源基因的载体。利用发根土壤杆菌介导。pRI101 DNA vectors are binary plant transformation vectors intended for foreign gene expression in plant cells. These agrobacterium-mediated plant transformation vectors carry the 35S promoter of caμliflower mosaic virus (CaMV) and the 5’ non-coding region (5’-UTR) of the alcohol dehydrogenase (ADH) gene. The 5’-UTR of ADH functions as a translation enhancer in plants. There are two types of pRI101 DNA vectors, the pRI101-AN DNA vector, which carries the 5’-UTR of Arabidopsis ADH (AtADH 5’-UTR), and the pRI101 ON DNA vector, which carries the 5’-UTR of rice ADH (OsADH 5’-UTR). The pRI101-AN DNA vector is for dicotyledonous plants such as tobacco or Arabidopsis while the pRI101-ON DNA vector is for monocotyledonous plants such as rice.Although the pRI101 DNA vectors are shuttle vectors and replicate autonomously in E. coli and Rhizobium (Agrobacterium), they are high copy number plasmids because they contain the same replication origin as pUC-type plasmids (ColE1 ori). These vectors are also stably maintained in Rhizobium (Agrobacterium) containing the mutant-type replication origin Ri (Ri-ori). The pRI101 DNA vectors are capable of stably integrating target genes into plant chromosomes because the vector cloning sites are located closer to the Right Border (RB) of T-DNA than the selection marker (NPT II) of the plant, so the target gene is not deleted.

质粒图谱



质粒序列

LOCUS       Exported               10417 bp ds-DNA     circular SYN 23-NOV-2017
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..10417
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(57..645)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6419..7213)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        7620..7650
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7658..7674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7682..7698
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9017..9033)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
       61 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      121 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      181 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      241 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      301 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      361 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      421 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      481 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      541 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      601 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      661 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      721 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      781 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      841 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactcga tcctacaagg
      901 tagaatccgc ctgagtcgca agggtgactt cgcctatatt ggacgacggc gcgcagaggg
      961 cgacctcttt ttgggttacg attgtaggat tatcactaaa acaatacatg aacatattca
     1021 aatggcaatc tctctaaggc attggaaata aatacaaata acagttgggt ggagtttttc
     1081 gacctgaggg cgttaacctt ctgttaacct aaaagctctt gcccaaacag cagaatcggc
     1141 gctaattgcc agcggcggaa cttttccagt ttcgcgaaaa atatcgccac tggcaaggaa
     1201 tgggtttgag atggcgaagt ctgtcctaaa agcagcgcct gtagttgtag ggttgacggc
     1261 cttgatggag cgtcatgccg atgccctctc gagccaactt caagcacatc atcttaaggt
     1321 tttcccgccg cattccgaga agggtattcg aacattcggg ccatcggagg cgtccaagct
     1381 gctcggcgtt ggcgagtcat atttacggca gaccgcgtct gagatgccag agttgaatgt
     1441 tagcatgagc ccaggtggca ggcgaatgtt ctcaattgaa gatatccatg tgattcggaa
     1501 gtatatggat caggtcggcc gcgggaaccg gcgctacctg ccacatcgtc gaggcggcga
     1561 gcagcttcag gttatctctg tgatgaattt caaaggtggg tcgggtaaga ccaccaccgc
     1621 cgcgcatctg gcgcagtacc tcgctatgcg cggatatcga gtcttggcca ttgatctcga
     1681 tcctcaagcg agcctttctg cactctttgg gagccaaccg gagacggacg ttggcccgaa
     1741 cgaaacgctc tacggcgcta taaggtatga tgatgagcag gtggcaatcg aacgagtcgt
     1801 ccgagggact tacattcccg acctccacct gattcctggt aaccttgagc tgatggagtt
     1861 tgaacacgat acgccacgcg cgctgatgaa ccgcaaagag ggcgacacgc tcttttatgg
     1921 tcgcatcagc caagtaattg aagatatcgc ggataactat gacgtcgtgg tcatcgactg
     1981 ccctccccag cttgggtatc tcacgctatc cgcattgact gcggcgacgt ccattcttgt
     2041 cacggtccat ccgcagatgc tggatgtgat gtcgatgaac cagtttctgg caatgacatc
     2101 gaaccttttg cgtgaaatcg agaatgctgg cgccaagttc aagtttaatt ggatgcgcta
     2161 tctgataacc cgtttcgaac cgagcgacgg accacagaac caaatggtag gttatctgcg
     2221 gtcgattttt ggcgaaaatg tcctcaattt tccgatgctt aaaaccaccg cggtttcgga
     2281 cgctggcctg acaaaccaga ctctattcga agtggagcgt ggcctgttca cgcgctcgac
     2341 ctatgatcga gccttggagg cgatgaacgc cgtcaacgac gagatcgaaa cactgatcaa
     2401 aaaagcatgg ggtaggccca catgagccgg aagcacatcc ttggcgtctc aactgacgcc
     2461 cctgagacgt cgcccgccga caataggacg gcaaagaacc gctccatgcc gctcctcggc
     2521 gtaacaagga aggagcgcga tccggcaacg aagctcacag cgaacattgg taacgcactg
     2581 cgagagcaaa acgatcgtct tagccgtgcc gaagagatcg agcggcgtct cgctgaaggt
     2641 caggcagtga tagagttgga tgcctcgtca atagaaccgt ctttcgtgca ggatcgtatg
     2701 cgaggggaca ttgacgggct ccttacttcg atccgggaac aaggacagca agtcccaatc
     2761 cttgtgcgac cgcatccgag ccagccgggc cgatatcagg ttgccttcgg ccaccgccgg
     2821 ctacgcgccg tttcagaact cggacttccg gtcagggcgg tcgttcgcga actgacggac
     2881 gagcaagtgg tcgtagcaca gggtcaggaa aacaatgtgc gcgaagatct taccttcatc
     2941 gaaaaggcgc gcttcgcaca tcgcctgaac aggcagtttt ctcgagagat tgtcatcgcc
     3001 gcgatgtcga tcgacaagag caatttgtcc aagatgcttc tgctcgttga cgccctcccc
     3061 tctgaactga ccgatgctat tggtgccgct cctggtgttg gacggccgag ttggcaacaa
     3121 cttgccgagc tgattgagaa agtttcttca ccggccgacg tggctaaata tgctatgtcg
     3181 gaggaagttc aagcgctgcc atcggcagaa cgattcaagg cggtgatcgc tagtctgaag
     3241 cccagtcggg ttgcgcgtgg acttcccgag gtcatggcca ccccagacgg caccagaatt
     3301 gcacaggtga cgcagagcaa ggccaaactg gaaatcacga ttgacaggaa ggcgacgccc
     3361 gattttgcga ccttcgtgct cgatcatgtg ccagcgctgt atcaagcgta ccacgctgag
     3421 aaccaacgga aacggggaga gtaaaccgca aaagaaaaga gccccctcaa cgtcgccgtc
     3481 gcggaagccc ttctgtctct ctagcgcgaa cagaatcgca tttcctcgaa tcctcgtcaa
     3541 gagtttttag cgccgttttg gtgagctgat ttcctttgcc tgctgaaagg tgaaagatga
     3601 tgcagacagg aagtgtaacg acgccattcg ggcggcggcc aatgacgctt gcgcttgtgc
     3661 ggcgccagac ggcgctggcc gatatcaaac aaggcaagac agcggacaag tggaaggtct
     3721 ttagggacgc gtccgcggcc atggaactac ttggaatcca gtccaacagt cttgccgtcc
     3781 ttgatgcgct attgagcttt cacccggaaa cggagttgcg tcaggaggca cagctgatcg
     3841 tcttcccgtc gaatgctcag cttgcccttc gggcgcatgg gatggctggc gcgactttgc
     3901 gtaggcacat cgccatgctc gtggagtcag gcttgatcgt ccggaaggat agcgccaacg
     3961 gaaagcgtta cgctcgtaag gatggcgctg gtcagatcga gcgcgcgttt ggcttcgatt
     4021 tgtctccgct tctcgcgcgg tccgaagagc tagcgatgat ggcacagcag gtgatggccg
     4081 atcgagcagc attcaggatg gccaaagaaa gtctgacgat ttgccgacgg gacgttcgga
     4141 agctaattac ggcagctatg gaagagggag cggagggcga ctggcaagct gtcgaggaag
     4201 tctatgtgga acttgtgggt agaatcccac gcgccccgac gcttgctgat gtagagtcaa
     4261 ttctcgaaga gatgtggatg ctccaggaag agataatcaa ccggttggaa attagagaca
     4321 attcagaaaa taatagcacc aatgctgccc agagcgagca gcacatacag aactcaaaac
     4381 ccgaatccgt taatgaactt gaacctcgct ctgaaaagga gcagggcgct aagccgagtg
     4441 aaatagaccg ggcaaggagc gagccgataa aagcgttccc cctcgggatg atcctgaaag
     4501 cgtgcccgac cattggcaat tatgggccga gcggtgcggt tgctagctgg cgtgacctca
     4561 tgtcggctgc ggtggtggtt cggtctatgc tgggggtcag cccgtcggct taccaagacg
     4621 cgtgtgaggc aatgggaccg gagaatgcgg cagcagcgat ggcgtgcatt ttggagcgag
     4681 cgaacttcat caattcgcct gggggctatc tccgagatct gacacggcgg agcgaactcg
     4741 ggaagttttc acttggcccg atgataatgg cgctcttgaa ggctagcggg caggggacgt
     4801 tgcggtttgg ctagaattag cgagtatgga gcaggatggt ctgtggtcag ctgaccacag
     4861 acctaatagg ttgaaaacat gagcgttttt tggatgatcg acagaccatc cgattcccgg
     4921 agtaccaagc gtgctctgat gggagcgata acattactca acaagcacga aggccccatg
     4981 ccgatcgttg atcgtgaagg agagcctgct ctacatgcgg cggtattttg ccggccgagg
     5041 catgtagtcg cggagcactg cctatttact gccctaggca caaacgttga ctcttggatc
     5101 gagctggcag acaaagcaat aacccacaca gaggacgatt aatggctgac gaagagatcc
     5161 agaatccgcc ggacggtact gctgctgccg aagttgagcc ggctgctcct agaggtagaa
     5221 gagcaaagaa agcaccagcc gaaacagccc gcacgggatc gttcaaatcc gtgaagccga
     5281 aaacccgcgg cctcagcaac cgagaaaaac tggagaagat cggtcaaatc gaagctcagg
     5341 tcgctggcgg cgcaaccttg aaggacgccg ttaagatcgt gggtatttcc gttcagacct
     5401 attatcaatg gaagagagct gcggttcaac ctgtctcaca gaatccggcc gtgtctgttt
     5461 cagttgacga tgaactcggc gagttcatcc aactcgagga ggaaaatcgg cggctcagaa
     5521 agctagcttg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
     5581 acacattgcg gacgttttta atgtactggg gtggtttttc ttttcaccag tgagacgggc
     5641 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     5701 gtttgcccca gcaggcgaaa atcctgtttg atggtggttc cgaaatcggc aaaatccctt
     5761 ataaatcaaa agaatagccc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     5821 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     5881 gcccaaattc ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
     5941 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
     6001 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
     6061 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
     6121 tttattgcca aatgtttgaa cgatcggggc ccgatcatat tgtcgctcag gatcgtggcg
     6181 ttgtgcttgt cggccgttgc tgtcgtaatg atatcggcac cttcgaccgc ctgttccgca
     6241 gagatcccgt gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg
     6301 gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa
     6361 tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc
     6421 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
     6481 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
     6541 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
     6601 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
     6661 cgagatcatc gccgtcgggc atacgcgcct tgagcctggc gaacagttcg gctggcgcga
     6721 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
     6781 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
     6841 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
     6901 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
     6961 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
     7021 ctgcctcgtc ctgtagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
     7081 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
     7141 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
     7201 cttgttcaat catgcgaaac gatccagatc cggtgcagat tatttggatt gagagtgaat
     7261 atgagactct aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca
     7321 agaaatattt gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc
     7381 tgacgtatgt gcttagctca ttaaactcca gaaacccgcg gctgagtggc tccttcaacg
     7441 ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat
     7501 aacgtgactc ccttaattct ccgctcatga tcctggcacg acaggtttcc cgactggaaa
     7561 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7621 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7681 acaggaaaca gctatgacca tgattacgaa ttaattcccg atctagtaac atagatgaca
     7741 ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa
     7801 tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca
     7861 tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac
     7921 cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga tcggggaaat
     7981 tcgagctcgg tagcaattcc cgaggctgta gccgacgatg gtgcgccagg agagttgttg
     8041 attcagaatt cggatccggt acccccgggg tcgacgggca tatgtatcaa cagtgaagaa
     8101 cttgcttttg atcttttgtt agttttgtgt gattgtgatg tatctagagt cccccgtgtt
     8161 ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat
     8221 tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg
     8281 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
     8341 ctgtcggcag aggcatcttc aacgatggcc tttcctttat cgcaatgatg gcatttgtag
     8401 gagccacctt ccttttccac tatcttcaca ataaagtgac agatagctgg gcaatggaat
     8461 ccgaggaggt ttccggatat taccctttgt tgaaaagtct caattgccct ttggtcttct
     8521 gagactgtat ctttgatatt tttggagtag acaagtgtgt cgtgctccac catgttgacg
     8581 aagattttct tcttgtcatt gagtcgtaag agactctgta tgaactgttc gccagtcttt
     8641 acggcgagtt ctgttaggtc ctctatttga atctttgact ccatggcctt tgattcagtg
     8701 ggaactacct ttttagagac tccaatctct attacttgcc ttggtttgtg aagcaagcct
     8761 tgaatcgtcc atactggaat agtacttctg atcttgagaa atatatcttt ctctgtgttc
     8821 ttgatgcagt tagtcctgaa tcttttgact gcatctttaa ccttcttggg aaggtatttg
     8881 atttcctgga gattattgct cgggtagatc gtcttgatga gacctgctgc gtaagcctct
     8941 ctaaccatct gtgggttagc attctttctg aaattgaaaa ggctaatctg gggacctgca
     9001 ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     9061 ttacccaact taatcgcctt gcagcacatc cccctttcgc caggatcaga ttgtcgtttc
     9121 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
     9181 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
     9241 tccatttgta tgtgactaaa acaattcatc cagtaaaata taatatttta ttttctccca
     9301 atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc
     9361 ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct
     9421 cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag
     9481 gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag
     9541 ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag
     9601 atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg
     9661 acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat
     9721 cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact
     9781 catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag
     9841 ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt
     9901 ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac
     9961 cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt
    10021 caattccggt gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat
    10081 ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact gttccttgca
    10141 ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat
    10201 aacatagtat cgacggagcc gattttgaaa ccacaattat gggtgatgct gccaacttac
    10261 tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc tatcagctgt
    10321 ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc cggacatcag
    10381 cgctatctct gctctcactg ccgtaaaaca tggcaac
//
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