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pRI201-ON单子叶植物发根双表达质粒

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产品名称: pRI201-ON单子叶植物发根双表达质粒
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产品展商: XYbscience
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简单介绍

pRI201-ON单子叶植物发根双表达质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。pRI201-ON单子叶植物发根双表达质粒收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。


pRI201-ON单子叶植物发根双表达质粒  的详细介绍

pRI201-ON单子叶植物发根双表达质粒

  • 产品信息
  • 基本信息
  • 质粒简介
  • 质粒图谱
  • 质粒序列

产品信息

产品货号 产品名称 产品规格 优惠价
XY1806 pRI201-ON单子叶植物发根双表达质粒

1μg

¥请加QQ询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子: 35S
原核抗性: 卡那霉素Kan
筛选标记: 遗传霉素G418
克隆菌株: 大肠杆菌HB101
培养条件: 37℃

质粒简介

       pRI201-ON是一个单子叶植物发根农杆菌的双表达质粒。The Ri Plasmid pRI201 is designed for the transformation and expression of target genes from plant cells. This series of binary vectors for plant transformation retains the backbone of the pRI101 vectors, including an alcohol dehydrogenase (ADH) gene-derived 5' untranslated region (5'-UTR) downstream of the cauliflower mosaic virus (CaMV promoter)-derived 35S promoter. Additionally, these vectors have a heat shock protein (HSP) gene-derived terminator in place of the nopaline synthase (NOS) gene-derived terminator, allowing higher target gene expression compared with the pRI101 vector series. Multigene transformation with a single vector can be achieved by integrating an expression cassette containing another gene (promoter + enhancer + gene of interest + terminator) into the vector's second multiple cloning site (MCS2) located downstream of the HSP terminator.
        The Ri Plasmid pRI201 binary vector series offers two types of vectors: pRI 201-AN DNA and pRI 201-ON DNA. pRI 201-AN DNA contains an Arabidopsis ADH-derived 5' UTR (AtADH 5'-UTR) and is suitable for dicotyledonous plant transformation. pRI 201-ON DNA contains a rice ADH-derived 5' UTR (OsADH 5'-UTR) and is suitable for monocotyledonous plant transformation. The pRI 201-AN DNA and pRI 201-ON DNA binary vectors for plant transformation have a mutant-type replication origin (Ri ori) from the Rhizobium rhizogenes Ri plasmid. These vectors also contain a replication origin (ColE1 ori) derived from the pUC plasmid; the ColE1 ori allows high copy number replication in E. coli. The vectors' multiple cloning site, located near the right border (RB) of T-DNA, allows stable target gene integration into the plant chromosome.

质粒图谱



质粒序列

LOCUS       Exported               10475 bp ds-DNA     circular SYN 23-NOV-2017
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..10475
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(57..645)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6419..7213)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        7620..7650
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7658..7674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7682..7698
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9075..9091)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
       61 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      121 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      181 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      241 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      301 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      361 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      421 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      481 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      541 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      601 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      661 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      721 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      781 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      841 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactcga tcctacaagg
      901 tagaatccgc ctgagtcgca agggtgactt cgcctatatt ggacgacggc gcgcagaggg
      961 cgacctcttt ttgggttacg attgtaggat tatcactaaa acaatacatg aacatattca
     1021 aatggcaatc tctctaaggc attggaaata aatacaaata acagttgggt ggagtttttc
     1081 gacctgaggg cgttaacctt ctgttaacct aaaagctctt gcccaaacag cagaatcggc
     1141 gctaattgcc agcggcggaa cttttccagt ttcgcgaaaa atatcgccac tggcaaggaa
     1201 tgggtttgag atggcgaagt ctgtcctaaa agcagcgcct gtagttgtag ggttgacggc
     1261 cttgatggag cgtcatgccg atgccctctc gagccaactt caagcacatc atcttaaggt
     1321 tttcccgccg cattccgaga agggtattcg aacattcggg ccatcggagg cgtccaagct
     1381 gctcggcgtt ggcgagtcat atttacggca gaccgcgtct gagatgccag agttgaatgt
     1441 tagcatgagc ccaggtggca ggcgaatgtt ctcaattgaa gatatccatg tgattcggaa
     1501 gtatatggat caggtcggcc gcgggaaccg gcgctacctg ccacatcgtc gaggcggcga
     1561 gcagcttcag gttatctctg tgatgaattt caaaggtggg tcgggtaaga ccaccaccgc
     1621 cgcgcatctg gcgcagtacc tcgctatgcg cggatatcga gtcttggcca ttgatctcga
     1681 tcctcaagcg agcctttctg cactctttgg gagccaaccg gagacggacg ttggcccgaa
     1741 cgaaacgctc tacggcgcta taaggtatga tgatgagcag gtggcaatcg aacgagtcgt
     1801 ccgagggact tacattcccg acctccacct gattcctggt aaccttgagc tgatggagtt
     1861 tgaacacgat acgccacgcg cgctgatgaa ccgcaaagag ggcgacacgc tcttttatgg
     1921 tcgcatcagc caagtaattg aagatatcgc ggataactat gacgtcgtgg tcatcgactg
     1981 ccctccccag cttgggtatc tcacgctatc cgcattgact gcggcgacgt ccattcttgt
     2041 cacggtccat ccgcagatgc tggatgtgat gtcgatgaac cagtttctgg caatgacatc
     2101 gaaccttttg cgtgaaatcg agaatgctgg cgccaagttc aagtttaatt ggatgcgcta
     2161 tctgataacc cgtttcgaac cgagcgacgg accacagaac caaatggtag gttatctgcg
     2221 gtcgattttt ggcgaaaatg tcctcaattt tccgatgctt aaaaccaccg cggtttcgga
     2281 cgctggcctg acaaaccaga ctctattcga agtggagcgt ggcctgttca cgcgctcgac
     2341 ctatgatcga gccttggagg cgatgaacgc cgtcaacgac gagatcgaaa cactgatcaa
     2401 aaaagcatgg ggtaggccca catgagccgg aagcacatcc ttggcgtctc aactgacgcc
     2461 cctgagacgt cgcccgccga caataggacg gcaaagaacc gctccatgcc gctcctcggc
     2521 gtaacaagga aggagcgcga tccggcaacg aagctcacag cgaacattgg taacgcactg
     2581 cgagagcaaa acgatcgtct tagccgtgcc gaagagatcg agcggcgtct cgctgaaggt
     2641 caggcagtga tagagttgga tgcctcgtca atagaaccgt ctttcgtgca ggatcgtatg
     2701 cgaggggaca ttgacgggct ccttacttcg atccgggaac aaggacagca agtcccaatc
     2761 cttgtgcgac cgcatccgag ccagccgggc cgatatcagg ttgccttcgg ccaccgccgg
     2821 ctacgcgccg tttcagaact cggacttccg gtcagggcgg tcgttcgcga actgacggac
     2881 gagcaagtgg tcgtagcaca gggtcaggaa aacaatgtgc gcgaagatct taccttcatc
     2941 gaaaaggcgc gcttcgcaca tcgcctgaac aggcagtttt ctcgagagat tgtcatcgcc
     3001 gcgatgtcga tcgacaagag caatttgtcc aagatgcttc tgctcgttga cgccctcccc
     3061 tctgaactga ccgatgctat tggtgccgct cctggtgttg gacggccgag ttggcaacaa
     3121 cttgccgagc tgattgagaa agtttcttca ccggccgacg tggctaaata tgctatgtcg
     3181 gaggaagttc aagcgctgcc atcggcagaa cgattcaagg cggtgatcgc tagtctgaag
     3241 cccagtcggg ttgcgcgtgg acttcccgag gtcatggcca ccccagacgg caccagaatt
     3301 gcacaggtga cgcagagcaa ggccaaactg gaaatcacga ttgacaggaa ggcgacgccc
     3361 gattttgcga ccttcgtgct cgatcatgtg ccagcgctgt atcaagcgta ccacgctgag
     3421 aaccaacgga aacggggaga gtaaaccgca aaagaaaaga gccccctcaa cgtcgccgtc
     3481 gcggaagccc ttctgtctct ctagcgcgaa cagaatcgca tttcctcgaa tcctcgtcaa
     3541 gagtttttag cgccgttttg gtgagctgat ttcctttgcc tgctgaaagg tgaaagatga
     3601 tgcagacagg aagtgtaacg acgccattcg ggcggcggcc aatgacgctt gcgcttgtgc
     3661 ggcgccagac ggcgctggcc gatatcaaac aaggcaagac agcggacaag tggaaggtct
     3721 ttagggacgc gtccgcggcc atggaactac ttggaatcca gtccaacagt cttgccgtcc
     3781 ttgatgcgct attgagcttt cacccggaaa cggagttgcg tcaggaggca cagctgatcg
     3841 tcttcccgtc gaatgctcag cttgcccttc gggcgcatgg gatggctggc gcgactttgc
     3901 gtaggcacat cgccatgctc gtggagtcag gcttgatcgt ccggaaggat agcgccaacg
     3961 gaaagcgtta cgctcgtaag gatggcgctg gtcagatcga gcgcgcgttt ggcttcgatt
     4021 tgtctccgct tctcgcgcgg tccgaagagc tagcgatgat ggcacagcag gtgatggccg
     4081 atcgagcagc attcaggatg gccaaagaaa gtctgacgat ttgccgacgg gacgttcgga
     4141 agctaattac ggcagctatg gaagagggag cggagggcga ctggcaagct gtcgaggaag
     4201 tctatgtgga acttgtgggt agaatcccac gcgccccgac gcttgctgat gtagagtcaa
     4261 ttctcgaaga gatgtggatg ctccaggaag agataatcaa ccggttggaa attagagaca
     4321 attcagaaaa taatagcacc aatgctgccc agagcgagca gcacatacag aactcaaaac
     4381 ccgaatccgt taatgaactt gaacctcgct ctgaaaagga gcagggcgct aagccgagtg
     4441 aaatagaccg ggcaaggagc gagccgataa aagcgttccc cctcgggatg atcctgaaag
     4501 cgtgcccgac cattggcaat tatgggccga gcggtgcggt tgctagctgg cgtgacctca
     4561 tgtcggctgc ggtggtggtt cggtctatgc tgggggtcag cccgtcggct taccaagacg
     4621 cgtgtgaggc aatgggaccg gagaatgcgg cagcagcgat ggcgtgcatt ttggagcgag
     4681 cgaacttcat caattcgcct gggggctatc tccgagatct gacacggcgg agcgaactcg
     4741 ggaagttttc acttggcccg atgataatgg cgctcttgaa ggctagcggg caggggacgt
     4801 tgcggtttgg ctagaattag cgagtatgga gcaggatggt ctgtggtcag ctgaccacag
     4861 acctaatagg ttgaaaacat gagcgttttt tggatgatcg acagaccatc cgattcccgg
     4921 agtaccaagc gtgctctgat gggagcgata acattactca acaagcacga aggccccatg
     4981 ccgatcgttg atcgtgaagg agagcctgct ctacatgcgg cggtattttg ccggccgagg
     5041 catgtagtcg cggagcactg cctatttact gccctaggca caaacgttga ctcttggatc
     5101 gagctggcag acaaagcaat aacccacaca gaggacgatt aatggctgac gaagagatcc
     5161 agaatccgcc ggacggtact gctgctgccg aagttgagcc ggctgctcct agaggtagaa
     5221 gagcaaagaa agcaccagcc gaaacagccc gcacgggatc gttcaaatcc gtgaagccga
     5281 aaacccgcgg cctcagcaac cgagaaaaac tggagaagat cggtcaaatc gaagctcagg
     5341 tcgctggcgg cgcaaccttg aaggacgccg ttaagatcgt gggtatttcc gttcagacct
     5401 attatcaatg gaagagagct gcggttcaac ctgtctcaca gaatccggcc gtgtctgttt
     5461 cagttgacga tgaactcggc gagttcatcc aactcgagga ggaaaatcgg cggctcagaa
     5521 agctagcttg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
     5581 acacattgcg gacgttttta atgtactggg gtggtttttc ttttcaccag tgagacgggc
     5641 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     5701 gtttgcccca gcaggcgaaa atcctgtttg atggtggttc cgaaatcggc aaaatccctt
     5761 ataaatcaaa agaatagccc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     5821 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     5881 gcccaaattc ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
     5941 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
     6001 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
     6061 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
     6121 tttattgcca aatgtttgaa cgatcggggc ccgatcatat tgtcgctcag gatcgtggcg
     6181 ttgtgcttgt cggccgttgc tgtcgtaatg atatcggcac cttcgaccgc ctgttccgca
     6241 gagatcccgt gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg
     6301 gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa
     6361 tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc
     6421 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
     6481 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
     6541 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
     6601 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
     6661 cgagatcatc gccgtcgggc atacgcgcct tgagcctggc gaacagttcg gctggcgcga
     6721 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
     6781 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
     6841 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
     6901 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
     6961 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
     7021 ctgcctcgtc ctgtagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
     7081 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
     7141 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
     7201 cttgttcaat catgcgaaac gatccagatc cggtgcagat tatttggatt gagagtgaat
     7261 atgagactct aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca
     7321 agaaatattt gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc
     7381 tgacgtatgt gcttagctca ttaaactcca gaaacccgcg gctgagtggc tccttcaacg
     7441 ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat
     7501 aacgtgactc ccttaattct ccgctcatga tcctggcacg acaggtttcc cgactggaaa
     7561 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7621 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7681 acaggaaaca gctatgacca tgattacgaa ttcccgggat ccagcggccg caggtaccaa
     7741 ttccttatct ttaatcatat tccatagtcc ataccatagc acatacagta gttatatgcg
     7801 aagagatcca acaaaacatt cacaatggat tatagaaaca tttgtttatt cattataatg
     7861 agatcttaca ttcatttaat attagaaaaa gccacaaatt cataacacaa caagccaaga
     7921 aaaaaacaca aacttaagca cacaagctag ctttttattt gacacaccaa atatttcatc
     7981 ttcatcttca taagagctcg gtagcaattc ccgaggctgt agccgacgat ggtgcgccag
     8041 gagagttgtt gattcagaat tgtcgacccg gtagtaactt gctaatcgac gggcatatgt
     8101 aatccccctc tttttcaaag aacaagaaac cactccgtag agatcgaaag ctcaggtttt
     8161 cttttttctt gaatcactcg cagttcgttg cttggaattc tctagagtcc cccgtgttct
     8221 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
     8281 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
     8341 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     8401 gtcggcagag gcatcttcaa cgatggcctt tcctttatcg caatgatggc atttgtagga
     8461 gccaccttcc ttttccacta tcttcacaat aaagtgacag atagctgggc aatggaatcc
     8521 gaggaggttt ccggatatta ccctttgttg aaaagtctca attgcccttt ggtcttctga
     8581 gactgtatct ttgatatttt tggagtagac aagtgtgtcg tgctccacca tgttgacgaa
     8641 gattttcttc ttgtcattga gtcgtaagag actctgtatg aactgttcgc cagtctttac
     8701 ggcgagttct gttaggtcct ctatttgaat ctttgactcc atggcctttg attcagtggg
     8761 aactaccttt ttagagactc caatctctat tacttgcctt ggtttgtgaa gcaagccttg
     8821 aatcgtccat actggaatag tacttctgat cttgagaaat atatctttct ctgtgttctt
     8881 gatgcagtta gtcctgaatc ttttgactgc atctttaacc ttcttgggaa ggtatttgat
     8941 ttcctggaga ttattgctcg ggtagatcgt cttgatgaga cctgctgcgt aagcctctct
     9001 aaccatctgt gggttagcat tctttctgaa attgaaaagg ctaatctggg gacctgcagg
     9061 catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     9121 acccaactta atcgccttgc agcacatccc cctttcgcca ggatcagatt gtcgtttccc
     9181 gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
     9241 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
     9301 catttgtatg tgactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat
     9361 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct
     9421 ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc
     9481 caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt
     9541 cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct
     9601 cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat
     9661 cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac
     9721 aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct
     9781 tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca
     9841 tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct
     9901 ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat
     9961 accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct
    10021 tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca
    10081 attccggtga tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt
    10141 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt
    10201 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa
    10261 catagtatcg acggagccga ttttgaaacc acaattatgg gtgatgctgc caacttactg
    10321 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
    10381 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg
    10441 ctatctctgc tctcactgcc gtaaaacatg gcaac
//
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