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pCXUN-FLAG植物pCX系列表达质粒

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产品名称: pCXUN-FLAG植物pCX系列表达质粒
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产品展商: XYbscience
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简单介绍

pCXUN-FLAG植物pCX系列表达质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。pCXUN-FLAG植物pCX系列表达质粒收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。


pCXUN-FLAG植物pCX系列表达质粒  的详细介绍

pCXUN-FLAG植物pCX系列表达质粒

  • 产品信息
  • 基本信息
  • 质粒简介
  • 质粒图谱
  • 质粒序列

产品信息

产品货号 产品名称 产品规格 优惠价
XY1631 pCXUN-FLAG植物pCX系列表达质粒

20μl

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使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

原核抗性: 卡那霉素Kan
筛选标记: 潮霉素Hyg
克隆菌株: 大肠杆菌DB3.1
培养条件: 37℃

质粒简介

          pCXUN-FLAG是一个植物pCX系列蛋白表达质粒。

质粒图谱

质粒序列

LOCUS       Exported               12035 bp ds-DNA     circular SYN 04-DEC-2017
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..12035
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      115..367
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             728..1033
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(1089..1112)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(1128..3118)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_feature    3359..3383
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4682..5311
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5745..6812
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6878..7072
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7416..7556
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7742..8330)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8417..9211)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9636..9660
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9738..9912
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9952..10977)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(11044..11721)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 gaattcgagc tcggtacccc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
       61 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcccgatcta
      121 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
      181 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
      241 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
      301 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
      361 aacgatcggg gaaattcgga tccccaatac ttgtatgggg cttactaaaa gccagataac
      421 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
      481 gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga
      541 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
      601 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
      661 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc
      721 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
      781 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
      841 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
      901 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
      961 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
     1021 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gacccataca
     1081 agtattggct tatcatcatc atccttgtaa tccatggatc ccccgggctg cagaagtaac
     1141 accaaacaac agggtgagca tcgacaaaag aaacagtacc aagcaaataa atagcgtatg
     1201 aaggcagggc taaaaaaatc cacatatagc tgctgcatat gccatcatcc aagtatatca
     1261 agatcaaaat aattataaaa catacttgtt tattataata gataggtact caaggttaga
     1321 gcatatgaat agatgctgca tatgccatca tgtatatgca tcagtaaaac ccacatcaac
     1381 atgtatacct atcctagatc gatatttcca tccatcttaa actcgtaact atgaagatgt
     1441 atgacacaca catacagttc caaaattaat aaatacacca ggtagtttga aacagtattc
     1501 tactccgatc tagaacgaat gaacgaccgc ccaaccacac cacatcatca caaccaagcg
     1561 aacaaaaagc atctctgtat atgcatcagt aaaacccgca tcaacatgta tacctatcct
     1621 agatcgatat ttccatccat catcttcaat tcgtaactat gaatatgtat ggcacacaca
     1681 tacagatcca aaattaataa atccaccagg tagtttgaaa cagaattcta ctccgatcta
     1741 gaacgaccgc ccaaccagac cacatcatca caaccaagac aaaaaaaagc atgaaaagat
     1801 gacccgacaa acaagtgcac ggcatatatt gaaataaagg aaaagggcaa accaaaccct
     1861 atgcaacgaa acaaaaaaaa tcatgaaatc gatcccgtct gcggaacggc tagagccatc
     1921 ccaggattcc ccaaagagaa acactggcaa gttagcaatc agaacgtgtc tgacgtacag
     1981 gtcgcatccg tgtacgaacg ctagcagcac ggatctaaca caaacacgga tctaacacaa
     2041 acatgaacag aagtagaact accgggccct aaccatggac cggaacgccg atctagagaa
     2101 ggtagagagg gggggggggg gaggacgagc ggcgtacctt gaagcggagg tgccgacggg
     2161 tggatttggg ggagatctgg ttgtgtgtgt gtgcgctccg aacaacacga ggttggggaa
     2221 agagggtgtg gagggggtgt ctatttatta cggcgggcga ggaagggaaa gcgaaggagc
     2281 ggtgggaaag gaatcccccg tagctgccgt gccgtgagag gaggaggagg ccgcctgccg
     2341 tgccggctca cgtctgccgc tccgccacgc atttctggat gccgacagcg gagcaagtcc
     2401 aacggtggag cggaactctc gagaggggtc cagaggcagc gacagagatg ccgtgccgtc
     2461 tgcttcgctt ggcccgacgc gacgctgctg gttcgctggt tggtgtccgt tagactcgtc
     2521 gacggcgttt aacaggctgg cattatctac tcgaaacaag aaaaatgttt ccttagtttt
     2581 tttaatttct taaagggtat ttgtttaatt tttagtcact ttattttatt ctattttata
     2641 tctaaattat taaataaaaa aactaaaata gagttttagt tttcttaatt tagaggctaa
     2701 aatagaataa aatagatgta ctaaaaaaat tagtctataa aaaccattaa ccctaaaccc
     2761 taaatggatg tactaataaa atggatgaag tattatatag gtgaagctat ttgcaaaaaa
     2821 aaaggagaac acatgcacac taaaaagata aaactgtaga gtcctgttgt caaaatactc
     2881 aattgtcctt tagaccatgt ctaactgttc atttatatga ttctctaaaa cactgatatt
     2941 attgtagtac tatagattat attattcgta gagtaaagtt taaatatatg tataaagata
     3001 gataaactgc acttcaaaca agtgtgacaa aaaaaatatg tggtaatttt ttataactta
     3061 gacatgcaat gctcattatc tctagagagg ggcacgaccg ggtcacgctg cactgcagga
     3121 attcgatatc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     3181 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     3241 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga
     3301 gcagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg
     3361 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt
     3421 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
     3481 ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt
     3541 ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg
     3601 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa
     3661 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg
     3721 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga
     3781 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga
     3841 ccgcccggag ctggccagga tgcttgacca cctagccctg gcgacgttgt gacagtgacc
     3901 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
     3961 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
     4021 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
     4081 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc
     4141 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
     4201 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
     4261 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
     4321 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
     4381 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
     4441 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc
     4501 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg
     4561 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
     4621 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
     4681 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
     4741 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
     4801 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
     4861 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
     4921 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
     4981 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gcaaccgccg acctggtgga
     5041 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
     5101 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
     5161 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
     5221 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
     5281 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
     5341 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
     5401 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
     5461 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
     5521 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
     5581 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
     5641 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc
     5701 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg
     5761 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     5821 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     5881 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     5941 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     6001 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     6061 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     6121 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     6181 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     6241 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     6301 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     6361 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     6421 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
     6481 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     6541 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     6601 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     6661 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     6721 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
     6781 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
     6841 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga
     6901 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
     6961 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
     7021 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
     7081 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
     7141 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
     7201 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
     7261 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     7321 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     7381 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     7441 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     7501 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     7561 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7621 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7681 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7741 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7801 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7861 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7921 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7981 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8041 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8101 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8161 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     8221 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8281 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8341 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8401 tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
     8461 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
     8521 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
     8581 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
     8641 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
     8701 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
     8761 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
     8821 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
     8881 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
     8941 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
     9001 gctttccttc cagccatagc atcatgtcct tttcccgttc aacatcatag gtggtccctt
     9061 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
     9121 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
     9181 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
     9241 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
     9301 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
     9361 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
     9421 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
     9481 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
     9541 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
     9601 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
     9661 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac
     9721 gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa
     9781 ttttattgat agaagtattt tacaaataca aatacatact aagggtttct tatatgctca
     9841 acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt
     9901 attatggaga aactcgagct tgtcgatcga cagatccggt cggcatctac tctatttctt
     9961 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
    10021 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
    10081 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
    10141 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg
    10201 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
    10261 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
    10321 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
    10381 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
    10441 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
    10501 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
    10561 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
    10621 catagcctcc gcgaccggtt gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa
    10681 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctaa actccccaat
    10741 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
    10801 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
    10861 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
    10921 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc
    10981 tcattgcccc ccggatctgc gaaagctcga gagagataga tttgtagaga gagactggtg
    11041 atttcagcgt gtcctctcca aatgaaatga acttccttat atagaggaag gtcttgcgaa
    11101 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
    11161 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
    11221 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
    11281 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
    11341 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
    11401 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
    11461 accatgttat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
    11521 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
    11581 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
    11641 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
    11701 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
    11761 ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag ccaatacgca
    11821 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    11881 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    11941 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    12001 aatttcacac aggaaacagc tatgaccatg attac
//
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