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pK7GWIWG2II-RedRoot

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产品名称: pK7GWIWG2II-RedRoot
产品型号:
产品展商: XYbscience
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简单介绍

pK7GWIWG2II-RedRoot菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。pK7GWIWG2II-RedRoot收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。


pK7GWIWG2II-RedRoot  的详细介绍

pK7GWIWG2II-RedRoot

  • 产品信息
  • 基本信息
  • 质粒简介
  • 质粒图谱
  • 质粒序列

产品信息

产品货号 产品名称 产品规格 优惠价
XY0686 pK7GWIWG2II-RedRoot

20μl

¥请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子: CaMV 35S
复制子: pVS1 oriV,ori
终止子: NOS
质粒分类: 植物系列,gateway载体
质粒大小: 15413bp
质粒标签: ccdB
原核抗性: Kan
筛选标记: Neo
克隆菌株: DB3.1
培养条件: 37℃,有氧 LB
表达宿主: 植物细胞
5'测序引物: 35S:GACGCACAATCCCACTATCC
3'测序引物: 根据序列设计引物

质粒简介

质粒图谱

质粒序列

LOCUS       Exported               15413 bp ds-DNA     circular SYN 20-JUL-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pK7GWIWG2 II-RedRoot
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, September 19, 2016 from SnapGene Viewer 3.2.1
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..15413
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      65..317
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    2772..2896
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2921..2951
                     /note="lac UV5 promoter"
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3303..3608
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /note="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3649..3773)
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4148..4807
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     protein_bind    5210..5334
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(5375..5680)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /note="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     promoter        complement(6032..6062)
                     /note="lac UV5 promoter"
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(6087..6211)
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(6320..6664)
                     /note="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    7357..7381
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8681..9310
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /note="pVS1 StaA"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9744..10811
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /note="pVS1 RepA"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      10877..11071
                     /note="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    11415..11555
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(11741..12329)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12573..13364)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="SmR"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    13892..13916
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      14009..14261
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(14301..15095)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(15129..15308)
                     /note="NOS promoter"
                     /note="nopaline synthase promoter"
ORIGIN
        1 ctagatgcat gctcgagcgg ccgccagtgt gatggatatc tgcagaattc gcccttgggc
       61 ccccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
      121 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
      181 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
      241 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
      301 caaatgtttg aacgatcggg aattggatcc tacaggaaca ggtggtggcg gccctcggtg
      361 cgctcgtact gctccacgat ggtgtagtcc tcgtttgtgg gaggtgatgt ccagcttgga
      421 gtccacgtag tagtagccgg gcagctgcac gggcttcttg gccatgtaga tggacttgaa
      481 ctccaccagg tagtggccgc cgtccttcag cttcagggcc ttgtggatct cgcccttcag
      541 cacgccgtcg cgggggtaca ggcgctcggt ggaggcctcc cagcccatgg tcttcttctg
      601 cattacgggg ccgtcggagg ggaagttcac gccgatgaac ttcaccttgt agatgaagca
      661 gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat
      721 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc ttgtagtcgg ggatgtcggc
      781 ggggtgcttc acgtacacct tggagccgta ctggaactgg ggggacagga tgtcccaggc
      841 gaagggcagg gggccgccct tggtcacctt cagcttcacg gtgttgtggc cctcgtaggg
      901 gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggtgc cctccatgcg
      961 caccttgaag cgcatgaact ccttgatgac gttcttggag gagcgcgcca tggcaaagat
     1021 ctgcatctgt taatcagaaa aactcagatt aatcgacaaa ttcgatcgca caaactagaa
     1081 actaacacca gatctagata gaaatcacaa atcgaagagt aattattcga caaaactcaa
     1141 attatttgaa caaatcggat gatatctatg aaaccctaat cgagaattaa gatgatatct
     1201 aacgatcaaa cccagaaaat cgtcttcgat ctaagattaa cagaatctaa accaaagaac
     1261 atatacgaaa ttgggatcga acgaaaacaa aatcgaagat tttgagagaa taaggaacac
     1321 agaaatttac cttgatcacg gtagagagaa ttgagagaaa gtttttaaga ttttgagaaa
     1381 ttgaaatctg aattgtgaag aagaagagct ctttgggtat tgttttatag aagaagaaga
     1441 agaaaagacg aggacgacta ggtcacgaga aagctaaggc ggtgaagcaa tagctaataa
     1501 taaaatgaca cgtgtattga gcgttgttta cacgcaaagt tgtttttggc taattgcctt
     1561 atttttaggt tgaggaaaag tatttgtgct ttgagttgat aaacacgact cgtgtgtgcc
     1621 ggctgcaacc actttgacgc cgtttattac tggacttcgt ccgacaacca caatttcctt
     1681 aacggtcgtc ataagatcca gccgttgaga tttaacgatc gttacgattt atattttttt
     1741 agcattatcg ttttattttt taaatatacg gtggagctga aaattggcaa taattgaacc
     1801 gtgggtccca ctgcattgaa gcgtatttcg tattttctag aattcttcgt gctttatttc
     1861 ttttcctttt tgtttttttt tgccatttat ctaatgcaag tgggcttata aaatcagtga
     1921 atttcttgga aaagtaactt ctttatcgta taacatattg tgaaattatc catttctttt
     1981 aattttttta gtgttattgg atatttttgt atgattattg atttgcatag gataatgact
     2041 tttgtatcaa gttggtgaac aagtctcgtt aaaaaaggca agtggtttgg tgactcgatt
     2101 tattcttgtt atttaattca tatatcaatg gatcttattt ggggcctggt ccatatttaa
     2161 cactcgtgtt cagtccaatg accaataata ttttttcatt aataacaatg taacaagaat
     2221 gatacacaaa acattctttg aataagttcg ctatgaagaa gggaacttat ccggtcctag
     2281 atcatcagtt catacaaacc tccatagagt tcaacatctt aaacaagaat atcctgatct
     2341 gaagaatgtg gaggctttag tcccttggat acttgggagg ctgtggaaga acagaaacga
     2401 gctggtgctt aaagggaggg aatttggaac caatgaggta ttagtaagga cacaagaaga
     2461 tgcagatgag tggattagaa ggaaagaggc tcagaatgta aggaaagcac caaccacaac
     2521 gacgtcgcat gcctgcaggt cactggattt tggttttagg aattagaaat tttattgata
     2581 gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg
     2641 aaaccctata agaaccctaa ttcccttatc tgggaactac tcacacatta ttctggagaa
     2701 aaatagagag agatagattt gtagagagag actggtgatt tttgcggact ctagcatggc
     2761 cgcgggatat cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata
     2821 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
     2881 atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg
     2941 ctcgtataat gtgtggattt tgagttagga tccggcttac taaaagccag ataacagtat
     3001 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     3061 atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct
     3121 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     3181 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     3241 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg
     3301 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     3361 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     3421 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     3481 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     3541 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     3601 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
     3661 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     3721 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgatatca
     3781 ctagtgcggc cgcctgcagg tcgaccatat ggtcgacctg caggcggccg cactagtgat
     3841 gctgttatgt tcagtgtcaa gctgacctgc aaacacgtta aatgctaaga agttagaata
     3901 tatgagacac gttaactggt atatgaataa gctgtaaata accgagtata aactcattaa
     3961 ctaatatcac ctctagagta taatataatc aaattcgaca atttgacttt caagagtagg
     4021 ctaatgtaaa atctttatat atttctacaa tgttcaaaga aacagttgca tctaaacccc
     4081 tatggccatc aaattcaatg aacgctaagc tgatccggcg agattttcag gagctaagga
     4141 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
     4201 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
     4261 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
     4321 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
     4381 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
     4441 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
     4501 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
     4561 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
     4621 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
     4681 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tctgtgatgg
     4741 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
     4801 ggcgtaaacg cgtggatcag cttaatatga ctctcaataa agtctcatac caacaagtgc
     4861 caccttattc aaccatcaag aaaaaagcca aaatttatgc tactctaagg aaaacttcac
     4921 taaagaagac gatttagagt gttttaccaa gaatttctgt catcttacta aacaactaaa
     4981 gatcggtgtg atacaaaacc taatctcatt aaagtttatg ctaaaataag cataatttta
     5041 cccactaagc gtgaccagat aaacataact cagcacacca gagcatatat attggtggct
     5101 caaatcatag aaacttacag tgaagacaca gaaagccgta agaagaggca agagtatgaa
     5161 accttacctc atcatttcca tgaggttgct tctgatcccg cgggatatca ccactttgta
     5221 caagaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     5281 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggtcgac
     5341 ctgcagactg gctgtgtata agggagcctg acatttatat tccccagaac atcaggttaa
     5401 tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg
     5461 agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca
     5521 ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg gccaggggga
     5581 tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca aacagacgat
     5641 aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagtc cctgttctcg
     5701 tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc ttcctgattt
     5761 tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt tgtgcttacc
     5821 agaccggaga tattgacatc atatatgcct tgagcaactg atagctgtcg ctgtcaactg
     5881 tcactgtaat acgctgcttc atagcacacc tctttttgac atacttcggg tatacatatc
     5941 agtatatatt cttataccgc aaaaatcagc gcgcaaatac gcatactgtt atctggcttt
     6001 tagtaagccg gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta
     6061 aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt
     6121 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     6181 tttctcgttc agcttttttg tacaaacttg tgatatcact agtgcggccg cctgcaggtc
     6241 gactagaata gtaaattgta atgttgtttg ttgtttgttt tgttgtggta attgttgtaa
     6301 aaatacggat cgtcctgcag tcctctccaa atgaaatgaa cttccttata tagaggaagg
     6361 gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca
     6421 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
     6481 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
     6541 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
     6601 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
     6661 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
     6721 gtcgtgctcc accatgttga cgaagatttt cttcttgtca ttgagtcgta aaagactctg
     6781 tatgaactgt tcgccagtct tcacggcgag ttctgttaga tcctcgatct gaatttttga
     6841 ctccatggcc tttgattcag taggaactac tttcttagag actccaatct ctattacttg
     6901 ccttggttta tgaagcaagc cttgaatcgt ccatactgga atagtacttc tgatcttgag
     6961 aaatatatct ttctctgtgt tcttgatgca gttagtcctg aatcttttga ctgcatcttt
     7021 aaccttcttg ggaaggtatt tgatctcctg gagattatta ctcgggtaga tcgtcttgat
     7081 gagacctgcc gcgtaggcct ctctaaccat ctgtgggtca gcattctttc tgaaattgaa
     7141 gaggctaatc ttctcattat cggtggtgaa catggtatcg tcaccttctc cgtcgaactt
     7201 tcttcctaga tcgtagagat agagaaagtc gtccatggtg atctccgggg caaaggagat
     7261 cagcttggct ctagtcgacc atatgggaga gctcaagctt agcttgagct tggatcagat
     7321 tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
     7381 cctaagagaa aagagcgttt attagaataa cggatattta aaagggcgtg aaaaggttta
     7441 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact
     7501 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
     7561 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt
     7621 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac
     7681 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc
     7741 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc
     7801 aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg
     7861 cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc
     7921 agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt
     7981 agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg
     8041 ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc
     8101 gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag
     8161 atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca
     8221 ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg
     8281 cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc
     8341 ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc
     8401 aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg
     8461 tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt
     8521 ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc
     8581 gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata
     8641 tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact
     8701 taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca
     8761 actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg
     8821 ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga
     8881 ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc
     8941 ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc
     9001 aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga
     9061 ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg
     9121 catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg
     9181 tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc
     9241 agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa
     9301 actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc
     9361 ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca
     9421 gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac
     9481 gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca
     9541 gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat
     9601 ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg
     9661 cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc
     9721 caagcccgag gaatcggcgt gacggtcgca aaccatccgg cccggtacaa atcggcgcgg
     9781 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca
     9841 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
     9901 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
     9961 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
    10021 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
    10081 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg
    10141 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
    10201 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
    10261 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
    10321 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
    10381 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
    10441 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
    10501 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
    10561 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
    10621 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
    10681 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
    10741 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
    10801 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
    10861 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
    10921 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
    10981 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
    11041 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc
    11101 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
    11161 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
    11221 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc
    11281 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
    11341 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
    11401 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
    11461 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
    11521 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
    11581 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    11641 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    11701 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    11761 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    11821 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    11881 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    11941 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    12001 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    12061 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    12121 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    12181 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    12241 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    12301 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    12361 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcatgat atatctccca
    12421 atttgtgtag ggcttattat gcacgcttaa aaataataaa agcagacttg acctgatagt
    12481 ttggctgtga gcaattatgt gcttagtgca tctaatcgct tgagttaacg ccggcgaagc
    12541 ggcgtcggct tgaacgaatt tctagctaga cattatttgc cgactacctt ggtgatctcg
    12601 cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct
    12661 tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg
    12721 cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg
    12781 taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc
    12841 gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga
    12901 tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc
    12961 agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca
    13021 ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg
    13081 tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa
    13141 gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg
    13201 gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag
    13261 ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc
    13321 tctgatagtt gagtcgatac ttcggcgatc accgcttccc ccatgatgtt taactttgtt
    13381 ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
    13441 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc aaaaaaacat
    13501 gtcataacaa gaagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
    13561 ggttctggac cagttgcgtg acggcagtta cgctacttgc attacagctt acgaaccgaa
    13621 cgaggcttat gtccactggg ttcgtgcccg aattgatcac aggcagcaac gctctgtcat
    13681 cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag
    13741 ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc
    13801 tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
    13861 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat
    13921 tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga attaacgccg
    13981 aattgaatta tcagcttgca tgccggtcga tctagtaaca tagatgacac cgcgcgcgat
    14041 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
    14101 gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt
    14161 acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
    14221 ttcaatctta agaaacttta ttgccaaatg tttgaacgat ctgcttgact ctagctagag
    14281 tccgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
    14341 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
    14401 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc
    14461 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
    14521 caggcatcgc cgtgggtcac gacgagatcc tcgccgtcgg gcatccgcgc cttgagcctg
    14581 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
    14641 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
    14701 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
    14761 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
    14821 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
    14881 gtggccagcc acgatagccg cgctgcctcg tcttggagtt cattcagggc accggacagg
    14941 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
    15001 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
    15061 ggagaacctg cgtgcaatcc atcttgttca atcatgcctc gatcgagttg agagtgaata
    15121 tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa
    15181 gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct
    15241 gacgtatgtg cttagctcat taaactccag aaacccgcgg ctgagtggct ccttcaacgt
    15301 tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata
    15361 acgtgactcc cttaattctc atgtatgata attcgagggt acccggggat cct
//
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