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pTet-On

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产品名称: pTet-On
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产品展商: XYbscience
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简单介绍

pTet-On菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。pTet-On收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。


pTet-On  的详细介绍

pTet-On

  • 产品信息
  • 基本信息
  • 质粒简介
  • 质粒图谱
  • 质粒序列

产品信息

产品货号 产品名称 产品规格 优惠价
XY0174 pTet-On ¥

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子: CMV
复制子: pUC ori
终止子: SV40 poly(A) signal,beta-globin polyA signal
质粒分类: 哺乳细胞,四环素调控系统载体
质粒大小: 7400bp
原核抗性: Amp
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
诱导方式: 四环素诱导
5'测序引物: CMV-F:CGCAAATGGGCGGTAGGCGTG
3'测序引物: 根据序列设计引物

质粒简介

     pTet-On® expresses the reverse tet-responsive transcriptional activator (rtTA) from the strong immediate early promoter of cytomegalovirus (PCMV). tTA is a fusion of amino acids 1–207 of the tet repressor (TetR) and the negatively charged C-terminal activation domain (130 amino acids) of the VP16 protein of herpes simplex virus. pTet-On is similar to pTet-Off except for four amino acid changes that convert the TetR to a rTetR, and consequently, tTA to rtTA. (pTet-Off also contains several silent mutations.) pTet-On was originally described as pUHD17-1neo by Gossen et al. (1995). pTet-On can be distinguished from pTet-Off by digestion with Hind III.

质粒图谱

质粒序列

LOCUS       Exported File           7400 bp ds-DNA    circular SYN 28-1-2015
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    Untitled
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2015-1-28 from SnapGene 2.0.1
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        89..468
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        469..672
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             774..1781
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the reverse tetracycline repressor 
                     rTetR with the C-terminal activation domain of herpes 
                     simplex virus VP16"
                     /note="rtTA"
                     /note="In the Tet-On(R) system, rtTA binds to the 
                     Tet-responsive element?and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLKGESWQDFLRNNAKSFRCALLSHRNGAKVHSDTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     polyA_signal    1792..1926
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2646..3234)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3405..4265)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4266..4370)
                     /gene="bla"
                     /note="AmpR promoter"
     polyA_signal    4483..4617
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(5042..5062)
                     /codon_start=1
                     /locus_tag="
                     "
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /note="
                     "
                     /protein_id="
                     "
                     /translation="PKKKRKV"
     intron          5192..5257
                     /note="small t intron"
                     /note="simian virus 40 (SV40) small t antigen intron"
     CDS             complement(5677..6471)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(6832..7161)
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      6846..6981
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
ORIGIN
        1 ctcgaggagc ttggcccatt gcatacgttg tatccatatc ataatatgta catttatatt
       61 ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa
      121 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      181 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      241 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      301 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      361 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      421 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      481 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      541 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      601 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      661 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      721 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt catatgtcta
      781 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg
      841 aaggtttaac aacccgtaaa ctcgcccaga agcttggtgt agagcagcct acactgtatt
      901 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc
      961 accatactca cttttgccct ttaaaagggg aaagctggca agatttttta cgcaataacg
     1021 ctaaaagttt tagatgtgct ttactaagtc atcgcaatgg agcaaaagta cattcagata
     1081 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac
     1141 aaggtttttc actagagaac gcgttatatg cactcagcgc tgtggggcat tttactttag
     1201 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta
     1261 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag
     1321 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat
     1381 gtgaaagtgg gtccgcgtac agccgcgcgc gtacgaaaaa caattacggg tctaccatcg
     1441 agggcctgct cgatctcccg gacgacgacg cccccgaaga ggcggggctg gcggctccgc
     1501 gcctgtcctt tctccccgcg ggacacacgc gcagactgtc gacggccccc ccgaccgatg
     1561 tcagcctggg ggacgagctc cacttagacg gcgaggacgt ggcgatggcg catgccgacg
     1621 cgctagacga tttcgatctg gacatgttgg gggacgggga ttccccgggt ccgggattta
     1681 ccccccacga ctccgccccc tacggcgctc tggatatggc cgacttcgag tttgagcaga
     1741 tgtttaccga tgcccttgga attgacgagt acggtgggta gggggcgcga ggatccagac
     1801 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     1861 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     1921 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     1981 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctgcaagc
     2041 ctcgtcgtcc tggccggacc acgctatctg tgcaaggtcc ccggccccgg acgcgcgctc
     2101 catgagcaga gcgcccgccg ccgaggcgaa gactcgggcg gcgccctgcc cgtcccacca
     2161 ggtcaacagg cggtaaccgg cctcttcatc gggaatgcgc gcgaccttca gcatcgccgg
     2221 catgtccccc tggcggacgg gaagtatcca gctcgaccaa gcttggcgag attttcagga
     2281 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     2341 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     2401 accgttcagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2461 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2521 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2581 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2641 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2701 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2761 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2821 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2881 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2941 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3001 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3061 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3121 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3181 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3241 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3301 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3361 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3421 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3481 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3541 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3601 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3661 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3721 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3781 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3841 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3901 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3961 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4021 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4081 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4141 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4201 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4261 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4321 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4381 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     4441 aggcgtatca cgaggccctt tcgtcttcac tcgaggtcga gggatccaga catgataaga
     4501 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     4561 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     4621 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     4681 agcaagtaaa acctctacaa atgtggtatg gctgattatg atctctagtc aaggcactat
     4741 acatcaaata ttccttatta acccctttac aaattaaaaa gctaaaggta cacaattttt
     4801 gagcatagtt attaatagca gacactctat gcctgtgtgg agtaagaaaa aacagtatgt
     4861 tatgattata actgttatgc ctacttataa aggttacaga atatttttcc ataattttct
     4921 tgtatagcag tgcagctttt tcctttgtgg tgtaaatagc aaagcaagca agagttctat
     4981 tactaaacac agcatgactc aaaaaactta gcaattctga aggaaagtcc ttggggtctt
     5041 ctacctttct cttctttttt ggaggagtag aatgttgaga gtcagcagta gcctcatcat
     5101 cactagatgg catttcttct gagcaaaaca ggttttcctc attaaaggca ttccaccact
     5161 gctcccattc atcagttcca taggttggaa tctaaaatac acaaacaatt agaatcagta
     5221 gtttaacaca ttatacactt aaaaatttta tatttacctt agagctttaa atctctgtag
     5281 gtagtttgtc caattatgtc acaccacaga agtaaggttc cttcacaaag atccggacca
     5341 aagcggccat cgtgcctccc cactcctgca gttcgggggc atggatgcgc ggatagccgc
     5401 tgctggtttc ctggatgccg acggatttgc actgccggta gaactccgcg aggtcgtcca
     5461 gcctcaggca gcagctgaac caactcgcga ggggatcgag cccggggtgg gcgaagaact
     5521 ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga
     5581 agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg
     5641 tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     5701 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     5761 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     5821 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     5881 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     5941 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     6001 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     6061 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     6121 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     6181 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     6241 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     6301 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     6361 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     6421 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     6481 tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa
     6541 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     6601 caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc
     6661 actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta
     6721 gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt
     6781 cctttagcag cccttgcgcc ctgagtgctt gcggcagcgt gaagctagct ttttgcaaaa
     6841 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     6901 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     6961 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     7021 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
     7081 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     7141 tccacaccct aactgacaca cattccacag ctgcctcgcg cgtttcggtg atgacggtga
     7201 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     7261 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     7321 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     7381 attgtactga gagtgcacct
//
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