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pTRE2pur

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产品名称: pTRE2pur
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产品展商: XYbscience
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简单介绍

pTRE2pur菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。pTRE2pur收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。


pTRE2pur  的详细介绍

pTRE2pur

  • 产品信息
  • 基本信息
  • 质粒简介
  • 质粒图谱
  • 质粒序列

产品信息

产品货号 产品名称 产品规格 优惠价
XY0172 pTRE2pur

20μl

¥请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子: Tet-responsive promoter
复制子: pUC ori
终止子: SV40 poly(A) signal,beta-globin polyA signal
质粒分类: 哺乳细胞,四环素调控系统载体
质粒大小: 5108bp
原核抗性: Amp
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
诱导方式: 四环素诱导
5'测序引物: Sv40-polyA-R:GAAATTTGTGATGCTATTGC
3'测序引物: 根据序列设计引物

质粒简介

     pTRE2pur is a response plasmid that expresses a gene of interest (Gene X) in Clontech's Tet-On® and Tet-Off® Gene Expression Systems and Tet-On and Tet-Off Cell Lines (1). The Tet Expression Systems and Cell Lines give researchers ready access to the tetracycline-regulated expression systems described by Gossen & Bujard (2; Tet-Off) and Gossen et al. (3; Tet-On).pTRE2pur contains an MCS immediately downstream of the Tet-responsive PhCMV*-1 promoter. cDNAs or genes inserted into the MCS will be responsive to the tTA and rtTA regulatory proteins in the Tet-Off and Tet-On systems, respectively. PhCMV*-1 contains the Tet response element (TRE), which consists of seven copies of the 19-bp tet operator sequence (tetO). TheTRE element is just upstream of the minimal CMV promoter (Pmin CMV), which lacks the enhancer that is part of the complete CMV promoter. Consequently, PhCMV*-1 is silent in the absence of binding of TetR or rTetR to the tetO sequences. Note that the cloned insert must have an initiation codon. In some cases the addition of a Kozak consensus ribosome binding site (4) may improve expression levels; however, many cDNAs have been efficiently expressed in Tet systems without the addition of a Kozak sequence. The puromycin resistance gene is used to directly select for stable transformants.

 

质粒图谱

质粒序列

LOCUS       Exported                5108 bp ds-DNA     circular SYN 05-SEP-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pTRE2pur
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, September 5, 2016 from SnapGene Viewer 3.1.4
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    15..285
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        318..356
                     /note="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          554..1126
                     /note="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     polyA_site      1323..1378
                     /note="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal"
     rep_origin      complement(1947..2535)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2706..3566)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3567..3671)
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        3776..4105
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      3956..4091
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             4198..4797
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    5027..5108
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
       61 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      121 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      181 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
      241 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
      301 ctcggtaccc gggtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt
      361 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
      421 accgggaccg atccagcctc cgcggccccg aattcgagct cggtacccgg ggatcctcta
      481 gtcagctgac gcgtgctagc gcggccgcat cgataagctt gtcgacgata tctctagagc
      541 tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa
      601 attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc
      661 cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca
      721 accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt
      781 gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct
      841 ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta
      901 gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa attctggctg
      961 gcgtggaaat attcttattg gtagaaacaa ctacaccctg gtcatcatcc tgcctttctc
     1021 tttatggtta caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc
     1081 gggcccctct gctaaccatg ttcatgcctt cttctctttc ctacagctcc tgggcaacgt
     1141 gctggttgtt gtgctgtctc atcattttgg caaagaattc actcctcagg tgcaggctgc
     1201 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
     1261 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     1321 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     1381 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     1441 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
     1501 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     1561 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     1621 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     1681 tgtccctctt ctcttatgga gatccctcga ctgcattaat gaatcggcca acgcgcgggg
     1741 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1801 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1861 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1921 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1981 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2041 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2101 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2161 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2221 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2281 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2341 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2401 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2461 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2521 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2581 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2641 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2701 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2761 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2821 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     2881 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2941 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3001 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3061 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3121 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3181 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3241 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3301 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     3361 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3421 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3481 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3541 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3601 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3661 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     3721 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca ctcgagtgtg
     3781 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3841 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3901 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     3961 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4021 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     4081 ttttggaggc ctaggctttt gcaaaaagct tgcatgcctg caggtcggcc gccacgaccg
     4141 gtgccgccac catcccctga cccacgcccc tgacccctca caaggagacg accttccatg
     4201 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc
     4261 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc
     4321 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     4381 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     4441 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     4501 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     4561 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     4621 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     4681 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     4741 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
     4801 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgg ctccgaccga
     4861 agccacccgg ggcggccccg ccgaccccgc acccgccccc gaggcccacc gactctagag
     4921 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     4981 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     5041 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5101 ttcactgc
//
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