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phospho-STAT3 (Tyr705)抗体

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产品名称: phospho-STAT3 (Tyr705)抗体
产品型号:
产品展商: XYbscience
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简单介绍

phospho-STAT3 (Tyr705)抗体*初被发现的两个蛋白(STAT1和STAT2)均参与干扰素α(IFN-α)和干扰素γ信号转导。此后,有几个额外的STAT蛋白被鉴定(STAT3、4、5A、5B、6)。由于生长因子或细胞因子信号传导,酪氨酸发生磷酸化。这种磷酸化导致STAT蛋白在细胞核中的二聚化和移位。phospho-STAT3 (Tyr705)抗体在某些情况下,这个过程是由JAK Kinases(Janus Kinases 1, 2,3)。这些蛋白质的*大激活,在特定的酪氨酸和丝氨酸残基磷酸化可能是1α,3, 4,和5。


phospho-STAT3 (Tyr705)抗体  的详细介绍

phospho-STAT3 (Tyr705)抗体特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而,抗体可通过与病毒或**的特异性结合,直接发挥中和病毒的作用。

产品编号xy- 1658R

英文名称phospho-STAT3 (Tyr705)

中文名称磷酸化信号转导和转录激活因子3抗体

别    名STAT3 (phospho Y705); p-STAT3 (phospho Y705); Phospho-Stat3(pTyr705); STAT3(Phospho-Tyr705); p-STAT3(Tyr705); Phosphorylated Stat3(pTyr705); p-Stat3; Acute Phase Response Factor; APRF; DNA binding protein APRF; FLJ20882; MGC16063; Signal Transductor and Activator of Transcription 3; STAT 3; STAT3_HUMAN.  

JournalPMIDIFApplicationPLoS One (2016)267458843.2340WBMolecular biology reports (2012)227298782.5060WB

说 明 书100ul  

产品类型磷酸化抗体

研究领域肿瘤  心血管  细胞生物  **学  信号转导  干细胞  细胞凋亡  转录调节因子  表观遗传学  

抗体来源Rabbit

克隆类型Polyclonal

phospho-STAT3 (Tyr705)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig,

产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 (石蜡切片需做抗原修复)

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量85kDa

细胞定位细胞核 细胞浆

性    状Lyophilized or Liquid

浓    度1mg/1ml

免 疫 原KLH conjugated Synthesised phosphopeptide derived from human STAT3 around the phosphorylation site of Tyr705:AP(p-Y)LK

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

phospho-STAT3 (Tyr705)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

产品介绍background:

STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon alpha (IFN alpha) and IFN gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5.

Specific functions of the various members of the STAT family are poorly understood. STAT3 has been shown to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are cJun and cyclic AMP responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock out mice was lethal at the early embryonic stage.

STAT1 and STAT2 are induced by IFN alpha and forn 1, a heterodimer which is part of the ISGF transcription factor complex. The STAT3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFNa, IL10, IL6, IL11, IL12, IL2, EGF, among others. STAT3 beta appears to be activated by both EGF and IL6, but STAT3 alpha is only activated by EGF. STAT3 binds to DNA as either a homodimer or as a heterodimer with STAT1. STAT3 interacts with the APRE acute phase response element) and SIE (csis inducible element). STAT4, predominantly expressed in testis and myeloid cells, is activated by ILl2. STAT5 is activated by prolactin and IL3. STAT6 participates in signaling pathways mediated by IL4.


Function:

Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.


Subcellular Location:

Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3.


Tissue Specificity:

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.


Post-translational modifications:

Tyrosine phosphorylated upon stimulation with EGF (By similarity). Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus.


DISEASE:

Defects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.


SWISS:

P40763


Gene ID:

6774


phospho-STAT3 (Tyr705)抗体antibody, Ab)是由效应B细胞(效应**B细胞)分泌,机体用于抵御外来物质,如病毒,**等抗原,结构呈“Y”字型的球状蛋白质,仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质,均被称作抗原,因此对于抗抗体(能够结合抗体的抗体)来说,抗体本身也是一种抗原物质。

   QQ图片20171030091318

phospho-STAT3 (Tyr705)抗体普通抗体重链和轻链的结构

重链结构:普通的**球蛋白具有2条重链(H链),分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型,对应的**球蛋白名称分别为IgMIgGIgAIgDIgE

轻链结构:  普通**球蛋白具有2条轻链(L链),分子质量约25kDa,有κ链和λ链两种亚型,这两种轻链决定了Ig的亚型类别(IgG1IgG2IgG3IgG4)。一个天然的Ig分子两条轻链总是相同的,但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同,正常人血清**球蛋白κ链:λ链约为21,而在小鼠的比例为201

2.2抗体Fab段和Fc

IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1Fc,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应;经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段(fragment of antigen bindingFab),相当于抗体分子的两个臂,由一个完整的轻链和重链的VHCH1结构域组成。Fc段为可结晶段(fragment crystallizableFc)相当于IgCH2CH3结构域,是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区,以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2CH3的区域,相当于Y字结构下面那一部分。

合格 FAM116A FAM116A蛋白抗体
合格 合格 合格 GRP75 G蛋白偶联受体75抗体
合格 FAM118A FAM118A蛋白抗体
合格 FAM118B FAM118B蛋白抗体
合格 FAM119A FAM119A蛋白抗体
合格 合格 FAM124A FAM124A蛋白抗体
合格 DDX19A ATP依赖解旋酶DDX19A抗体
合格 FAM131B FAM131B蛋白抗体
合格 FAM131A FAM131A蛋白抗体
合格 FAM132A 脂肪源性胰岛素增敏因子抗体
合格 FAM135A FAM135A蛋白抗体
合格 FAM136a FAM136a蛋白抗体
合格 合格 FAM149B1 FAM149B1蛋白抗体
合格 合格 FAM151B FAM151B蛋白抗体
合格 FAM154B FAM154B蛋白抗体
合格 FAM160B1 FAM160B1蛋白抗体
合格 FAM161B FAM161B蛋白抗体
合格 FAM167A FAM167A蛋白抗体
合格 CCL28 粘膜相关上皮趋化因子28抗体
合格 FAM171A1 FAM171A1蛋白抗体
合格 FAM171A2 FAM171A2蛋白抗体
合格 Gremlin 骨形态形成蛋白拮抗蛋白抗体
合格 FAM189A1 FAM189A1蛋白抗体
 


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