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phospho-VEGF receptor 2 (Tyr1059)抗体

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产品名称: phospho-VEGF receptor 2 (Tyr1059)抗体
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产品展商: XYbscience
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简单介绍

phospho-VEGF receptor 2 (Tyr1059)抗体血管内皮生长因子(VEGF)是内皮细胞的主要生长因子。该基因编码VEGF的两个受体之一。这种受体被称为激酶插入域受体,是一种Ⅲ型受体酪氨酸激酶。它是VEGF诱导内皮细胞增殖、存活、迁移、管状形态发生和萌发的主要介质。信号和该受体运输是受多重因素影响,包括Rab GTPase,P2Y嘌呤核苷酸受体整合素α、细胞蛋白酪氨酸磷酸酶,等。phospho-VEGF receptor 2 (Tyr1059)抗体该基因突变与婴幼儿****瘤有关。[由RefSeq提供,2009年5月]。


phospho-VEGF receptor 2 (Tyr1059)抗体  的详细介绍

phospho-VEGF receptor 2 (Tyr1059)抗体特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而,抗体可通过与病毒或**的特异性结合,直接发挥中和病毒的作用。

产品编号xy- 3467R

英文名称phospho-VEGF receptor 2 (Tyr1059)

中文名称磷酸化血管内皮生长因子受体2抗体

别    名VEGFR2(Phospho-Tyr1059); VEGFR2(Phospho-Y1059); p-VEGFR2(Tyr1059); p-VEGFR2(Y1059); CD309; CD309 antigen; Fetal liver kinase 1; FLK-1; FLK1; KDR; Kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein tyrosine kinase receptor FLK1; Protein-tyrosine kinase receptor flk-1; Tyrosine kinase growth factor receptor; Vascular endothelial growth factor receptor 2; VEGFR 2; VEGFR; VEGFR-2; VEGFR2; VGFR2_HUMAN.  

说 明 书100ul  

产品类型磷酸化抗体

研究领域肿瘤  心血管  细胞生物  **学  信号转导  生长因子和**  转录调节因子  血管内皮细胞  

抗体来源Rabbit

克隆类型Polyclonal

phospho-VEGF receptor 2 (Tyr1059)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,

产品应用WB=1:500-2000 ELISA=1:500-1000 (石蜡切片需做抗原修复)

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量147kDa

细胞定位细胞核 细胞浆 细胞膜 分泌型蛋白

性    状Lyophilized or Liquid

浓    度1mg/1ml

免 疫 原KLH conjugated Synthesised phosphopeptide derived from human VEGF receptor 2 around the phosphorylation site of Tyr1059:PD(p-Y)VR

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

phospho-VEGF receptor 2 (Tyr1059)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

产品介绍background:

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009].


Function:

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA-and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.


Subunit:

Interacts with MYOF (By similarity). Interacts with VEGFA, VEGFC and VEGFD. Monomer in the absence of bound VEGFA, VEGFC or VEGFD. Homodimer in the presence of bound dimeric VEGFA, VEGFC or VEGFD. Can also form heterodimers with FLT1 and FLT4. Interacts (tyrosine phosphorylated) with FYN, NCK1, PLCG1 and SHB. Interacts with HIV-1 Tat. Interacts with CBL. Interacts with SH2D2A/TSAD and GRB2.


Subcellular Location:

Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome. Note=Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus.

Isoform 2: Secreted (Probable).

Isoform 3: Secreted.


Tissue Specificity:

Detected in cornea (at protein level). Widely expressed.


Post-translational modifications:

N-glycosylated.

Ubiquitinated. Tyrosine phosphorylation of the receptor promotes its poly-ubiquitination, leading to its degradation via the proteasome or lysosomal proteases.

Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-951 is important for interaction with SH2D2A/TSAD and VEGFA-mediated reorganization of the actin cytoskeleton. Phosphorylation at Tyr-1175 is important for interaction with PLCG1 and SHB. Phosphorylation at Tyr-1214 is important for interaction with NCK1 and FYN. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.


DISEASE:

Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.

Note=Plays a major role in tumor angiogenesis. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.


Similarity:

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Contains 7 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 protein kinase domain.


SWISS:

P35968


Gene ID:

3791

phospho-VEGF receptor 2 (Tyr1059)抗体antibody, Ab)是由效应B细胞(效应**B细胞)分泌,机体用于抵御外来物质,如病毒,**等抗原,结构呈“Y”字型的球状蛋白质,仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质,均被称作抗原,因此对于抗抗体(能够结合抗体的抗体)来说,抗体本身也是一种抗原物质。

   QQ图片20171030091318

phospho-VEGF receptor 2 (Tyr1059)抗体普通抗体重链和轻链的结构

重链结构:普通的**球蛋白具有2条重链(H链),分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型,对应的**球蛋白名称分别为IgMIgGIgAIgDIgE

轻链结构:  普通**球蛋白具有2条轻链(L链),分子质量约25kDa,有κ链和λ链两种亚型,这两种轻链决定了Ig的亚型类别(IgG1IgG2IgG3IgG4)。一个天然的Ig分子两条轻链总是相同的,但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同,正常人血清**球蛋白κ链:λ链约为21,而在小鼠的比例为201

2.2抗体Fab段和Fc

IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1Fc,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应;经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段(fragment of antigen bindingFab),相当于抗体分子的两个臂,由一个完整的轻链和重链的VHCH1结构域组成。Fc段为可结晶段(fragment crystallizableFc)相当于IgCH2CH3结构域,是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区,以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2CH3的区域,相当于Y字结构下面那一部分。

合格 BBS4 巴尔得-别德尔综合征相关蛋白4抗体
合格 muscarinic Acetylcholine Receptor 1 毒蕈碱型乙酰胆碱受体M1抗体
合格 合格 TTC8 巴尔得-别德尔综合征相关蛋白8抗体
合格 BBS9 巴尔得-别德尔综合征相关蛋白9抗体
合格 BBS10 巴尔得-别德尔综合征相关蛋白10抗体
合格 CCKAR 胆囊收缩素A受体抗体
合格 TRF1 端粒体复制结合因子1抗体
合格 合格 NHLH2 螺旋环螺旋蛋白2抗体 
合格 GALR2 甘丙肽受体2抗体
合格 ATPase Na+/ K+ beta 2 钠钾ATP酶通道蛋白抗体
合格 LAR 受体蛋白酪氨酸磷酸酶F抗体
合格 NPW/Neuropeptide W 神经肽W抗体
合格 PTPRN 蛋白酪氨酸磷酸酶样N前体蛋白抗体
合格 QRFP 神经***肽QRFP抗体
合格 合格 SLC27A4 长链脂肪酸转运蛋白4抗体
合格 Bid BH3结构域凋亡诱导蛋白抗体
合格 EN2 同源盒蛋白转录因子EN2抗体
合格 phospho-ERK5 (Thr218 + Tyr220) 磷酸化细胞外信号调节激酶5抗体
合格 FAM126A 髓鞘缺陷相关蛋白抗体
合格 FOXB1 + FOXB2 叉头蛋白B1+B2抗体
合格 Gemin 1 脊髓性肌萎缩症蛋白SMA/抗体
合格 Gli2 转录因子Gli2抗体
合格 Gli3 转录因子Gli3抗体 
合格 KIBRA 肾脏和脑蛋白抗体
 


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