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PIM3抗体

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产品名称: PIM3抗体
产品型号:
产品展商: XYbscience
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简单介绍

PIM3抗体Pim家族的丝氨酸/苏氨酸蛋白激酶研究的靶基因在小鼠白血病病毒诱导的T细胞**瘤病毒插入的首次发现。Pim激酶水平的增加导致细胞**瘤和增强有丝分裂蛋白如P100,基因,和CDC25A的活动。PIM3抗体此外,Pim激酶也参与神经元突触强度和抗造血祖细胞凋亡信号的调制。Pim-3,成员Pim家族的原癌基因表达的丝氨酸/苏氨酸激酶活性,显著的同源性,与股票中的丝氨酸/苏氨酸蛋白激酶。Pim-3可能充当调解员在大脑中的突触可塑性,以及对人肝癌细胞株的增殖可能参与抗凋亡过程中细胞周期进程。Pim-3蛋白广泛表达,但不表达在结肠癌、小肠和胸腺中观察到。


PIM3抗体  的详细介绍

PIM3抗体特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而,抗体可通过与病毒或**的特异性结合,直接发挥中和病毒的作用。

产品编号xy- 20023R

英文名称PIM3

中文名称丝氨酸/苏氨酸蛋白激酶PIM3抗体

别    名ABP1; Actin-binding protein 1; Cervical mucin associated protein; Cervical mucin-associated protein; Cervical SH3P7; CMAP; Dbnl; PIM3_HUMAN; DBNL_HUMAN; Drebrin F; drebrin like; Drebrin like protein; Drebrin-F; Drebrin-like protein; HIP 55; HIP-55; HPK1 interacting protein of 55 kDa; HPK1-interacting protein of 55 kDa; SH3 domain containing protein 7; SH3 domain-containing protein 7; SH3P7; Src Homology 3 Domain Containing Protein; src homology 3 domain containing protein HIP.  

说 明 书100ul  200ul

研究领域信号转导  转运蛋白  

抗体来源Rabbit

克隆类型Polyclonal

PIM3抗体交叉反应 Mouse, Rat,

产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量36kDa

细胞定位细胞浆

性    状Lyophilized or Liquid

浓    度1mg/1ml

免 疫 原KLH conjugated synthetic peptide derived from human PIM3:1-100/326

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

PIM3抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

产品介绍background:

The Pim family serine/threonine protein kinases were first identified in studies examining genes targeted for proviral insertion in murine leukemia virus-induced T lymphomas. Increased levels of Pim kinases predispose cells to lymphomagenesis and enhance the activity of mitogenic proteins such as p100, c-Myb, and Cdc25A. In addition, Pim kinases are also involved in modulation of synaptic strength in neurons and anti-apoptotic signaling in hematopoietic progenitor cells. Pim-3, a member of the proto-oncogene Pim family that expresses serine/threonine kinase activity, shares significant homology with Pim-1 serine/threonine protein kinases. Pim-3 may function as a mediator of synaptic plasticity in the brain and is presumably involved in the anti-apoptosis process and cell cycle progression as well as the proliferation of human hepatoma cell lines. The Pim-3 protein is widely expressed, however no expression is observed in the colon, thymus, or small intestine.


Function:

Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.


Subunit:

Interacts with BAD. Interacts with PPP2CA; this interaction promotes dephosphorylation of PIM3, ubiquitination and proteasomal degradation. Interacts with SOCS6.


Subcellular Location:

Cytoplasm.


Tissue Specificity:

Detected in various tissues, including the heart, brain, lung, kidney, spleen, placenta, skeletal muscle, and peripheral blood leukocytes. Not found or barely expressed in the normal ***** endoderm-derived organs such as colon, thymus, liver, or small intestine. However, expression is augmented in premalignant and malignant lesions of these organs.


Post-translational modifications:

Ubiquitinated, leading to proteasomal degradation.

Phosphorylated. Interaction with PPP2CA promotes dephosphorylation.


Similarity:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.

Contains 1 protein kinase domain.


Gene ID:

223775

PIM3抗体antibody, Ab)是由效应B细胞(效应**B细胞)分泌,机体用于抵御外来物质,如病毒,**等抗原,结构呈“Y”字型的球状蛋白质,仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质,均被称作抗原,因此对于抗抗体(能够结合抗体的抗体)来说,抗体本身也是一种抗原物质。

   QQ图片20171030091318

PIM3抗体普通抗体重链和轻链的结构

重链结构:普通的**球蛋白具有2条重链(H链),分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型,对应的**球蛋白名称分别为IgMIgGIgAIgDIgE

轻链结构:  普通**球蛋白具有2条轻链(L链),分子质量约25kDa,有κ链和λ链两种亚型,这两种轻链决定了Ig的亚型类别(IgG1IgG2IgG3IgG4)。一个天然的Ig分子两条轻链总是相同的,但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同,正常人血清**球蛋白κ链:λ链约为21,而在小鼠的比例为201

2.2抗体Fab段和Fc

IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1Fc,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应;经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段(fragment of antigen bindingFab),相当于抗体分子的两个臂,由一个完整的轻链和重链的VHCH1结构域组成。Fc段为可结晶段(fragment crystallizableFc)相当于IgCH2CH3结构域,是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区,以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2CH3的区域,相当于Y字结构下面那一部分。

合格 G-CSF/CSF3 粒细胞-巨噬细胞集落刺激因子3抗体
合格 CRHR1 促肾上腺皮质释放**受体1抗体
合格 合格 MCT1 单羧酸转运蛋白-1抗体
合格 MMP13 基质金属蛋白酶13抗体
合格 合格 MHC class I/HLA-C 组织相容性复合体蛋白1抗体
合格 合格 合格 COX4 细胞色素c氧化酶IV亚型1抗体
合格 Desmin 结蛋白抗体
合格 phospho-VAV3(Tyr141) 磷酸化鸟嘌呤核苷酸交换因子VAV3抗体
合格 合格 NSE 神经元特异性烯醇化酶/γ 烯醇化酶抗体
合格 NF-L 低分子量神经丝蛋白抗体
合格 REA B**细胞受体相关蛋白抗体
合格 合格 COMP 软骨寡聚基质蛋白抗体
合格 C5R1 补体成分5受体1抗体
合格 Cytokeratin 19 细胞角蛋白19抗体
合格 TREM1 髓系细胞触发受体1抗体
合格 Cenexin1 精子尾部结构蛋白抗体
合格 CD22 B**细胞粘附分子CD22抗体
合格 VNN1 血管非炎症分子1抗体
合格 IFITM1 干扰素诱导跨膜蛋白1抗体
合格 phospho-HDAC4 (Ser632) 磷酸化组蛋白去乙酰化酶4抗体
合格 Inhibin Alpha 抑制素A/Inhibin α抗体
合格 Factor XII light chain 凝血因子12轻链抗体
合格 Factor I heavy chain 补体因子I重链抗体
 


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