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PPP4C抗体

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产品名称: PPP4C抗体
产品型号:
产品展商: XYbscience
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简单介绍

PPP4C抗体蛋白磷酸酶,涉及许多过程如在中心体微管组织,成熟的剪接snRNPs,凋亡,DNA修复,肿瘤坏死因子(TNF)-α信号,c-Jun氨基末端激酶异常活化,调节组蛋白乙酰化,DNA损伤检验点信号,B激活和细胞迁移。的ppp4c-ppp4r1 PP4复合物可能在去磷酸化调控HDAC3发挥作用。的ppp4c-ppp4r2-ppp4r3a PP4复杂专门去磷酸化在H2AFX ser-140(gamma-h2afx)过程中产生的DNA复制和DNA双链断裂修复的要求。PPP4C抗体使NDEL1在Cdk1磷酸化位点和负向调节CDK1活性在间期(相似性)。在DNA损伤反应中,催化去磷酸化RPA2,至关重要的一步,因为它允许DNA修复的有效RPA2招聘RAD51染色质介导的。


PPP4C抗体  的详细介绍

PPP4C抗体特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而,抗体可通过与病毒或**的特异性结合,直接发挥中和病毒的作用。

产品编号xy- 6428R

英文名称PPP4C

中文名称丝氨酸/苏氨酸蛋白磷酸酶4C抗体

别    名PP X; PP4C; Protein phosphatase X; PP-X; PP4; PP4C; PP4C_HUMAN; PPH3; PPP4; ppp4c; PPX; protein phosphatase 4 (formerly X), catalytic subunit; Protein phosphatase 4 catalytic subunit; Protein phosphatase X; protein phosphatase X, catalytic subunit; Serine/threonine protein phosphatase 4 catalytic subunit; Serine/threonine-protein phosphatase 4 catalytic subunit.  

说 明 书100ul  200ul

研究领域细胞生物  **学  信号转导  激酶和磷酸酶  

抗体来源Rabbit

克隆类型Polyclonal

PPP4C抗体交叉反应 Human, Mouse, Rat, Cow, Rabbit,

产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量35kDa

细胞定位细胞核 细胞浆

性    状Lyophilized or Liquid

浓    度1mg/1ml

免 疫 原KLH conjugated synthetic peptide derived from human PPP4C

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

PPP4C抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

产品介绍background:

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.


Function:

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.


Subunit:

Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complexes PPP4C-PPP4R1, PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A, PPP4C-PPP4R2-PPP4R3B and PPP4C-PPP4R4. The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2. Interacts with REL, NFKB1/p50 and RELA. Interacts with SMN1 AND GEMIN4. Interacts with IRS4 (phosphorylated). Interacts with SMEK1/PPP4R3A; the interaction requires PP4R2. Interacts with HDAC3.


Subcellular Location:

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome.


Similarity:

Belongs to the PPP phosphatase family. PP-4 (PP-X).


SWISS:

P60510


Gene ID:

5531

PPP4C抗体antibody, Ab)是由效应B细胞(效应**B细胞)分泌,机体用于抵御外来物质,如病毒,**等抗原,结构呈“Y”字型的球状蛋白质,仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质,均被称作抗原,因此对于抗抗体(能够结合抗体的抗体)来说,抗体本身也是一种抗原物质。

   QQ图片20171030091318

PPP4C抗体普通抗体重链和轻链的结构

重链结构:普通的**球蛋白具有2条重链(H链),分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型,对应的**球蛋白名称分别为IgMIgGIgAIgDIgE

轻链结构:  普通**球蛋白具有2条轻链(L链),分子质量约25kDa,有κ链和λ链两种亚型,这两种轻链决定了Ig的亚型类别(IgG1IgG2IgG3IgG4)。一个天然的Ig分子两条轻链总是相同的,但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同,正常人血清**球蛋白κ链:λ链约为21,而在小鼠的比例为201

2.2抗体Fab段和Fc

IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1Fc,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应;经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段(fragment of antigen bindingFab),相当于抗体分子的两个臂,由一个完整的轻链和重链的VHCH1结构域组成。Fc段为可结晶段(fragment crystallizableFc)相当于IgCH2CH3结构域,是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区,以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2CH3的区域,相当于Y字结构下面那一部分。

合格 合格 p75 NGF Receptor 神经生长因子受体抗体
合格 MUC5AC 胃粘液素抗体
合格 ZNF238 锌指蛋白238抗体
合格 SNAI3 锌指蛋白293抗体
合格 ZNF408 锌指蛋白408抗体
合格 ZNF419 锌指蛋白419抗体
合格 SUSD4 SUSD4蛋白抗体
合格 SUSD5 SUSD5蛋白抗体
合格 GSK3 Alpha + Beta 糖原合酶激酶3α+β抗体
合格 SIRT2 沉默调节相关蛋白2抗体
合格 合格 CD200R CD200受体抗体
合格 DR5 死亡受体5抗体
合格 ERG3 癌基因ERG3抗体
合格 Melan A 黑色素瘤相关抗原/黑色素-A抗体
合格 phospho-CREB-1 (Thr100) 磷酸化CREB-1抗体
合格 Pepsinogen I 胃蛋白酶原1抗体
合格 Thymidine kinase 1 胸苷激酶1抗体
合格 beta Defensin 3 β防御素3抗体
合格 Histone H4 (tri methyl K20) 组蛋白H4(三甲基K20)抗体
合格 Pantophysin 突触素样蛋白1抗体
合格 DNA polymerase delta DNA聚合酶δ 抗体
合格 TBC1D15 TBC结构域家族D15蛋白抗体
合格 TBX18 TBX18蛋白抗体
合格 TGFBI 角膜上皮蛋白TGFBI抗体
 


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