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Raptor抗体

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产品名称: Raptor抗体
产品型号:
产品展商: XYbscience
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简单介绍

Raptor抗体mTOR控制细胞生长,部分通过调节核糖体蛋白S6激酶α(p70alpha)和真核起始因子4E结合蛋白1(4EBP1)。猛禽是一个150 kDa的mTOR蛋白结合并使4EBP1和p70alpha。猛禽以mTOR为mTOR催化磷酸化4EBP1体外是必要的,它强烈增强mTOR激酶活性对p70alpha。雷帕霉素或氨基酸撤出增加,而胰岛素强烈抑制,对7-甲基-GTP Sepharose恢复4EBP1和猛禽。RNA干扰抑制局部猛禽表达(RNAi)降低mTOR催化4EBP1磷酸化的影响。秀丽隐杆线虫的RNAi产生了一系列类似于失活产生的表型。Raptor抗体因此,猛禽的mTOR催化磷酸化4EBP1必不可少的支架和介导的Tor的体内作用。


Raptor抗体  的详细介绍

Raptor抗体特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而,抗体可通过与病毒或**的特异性结合,直接发挥中和病毒的作用。

产品编号xy- 3559R

英文名称Raptor

中文名称mTOR相关调控蛋白抗体

别    名p150 target of rapamycin (TOR) scaffold protein containing WD repeats; P150 target of rapamycin (TOR)-scaffold protein; Regulatory Associated Protein of mTOR; KIAA1303; RPTOR_HUMAN; Regulatory-associated protein of mTOR; p150 target of rapamycin (TOR)-scaffold protein.  

说 明 书100ul  200ul

研究领域肿瘤  **学  信号转导  细胞凋亡  转录调节因子  

抗体来源Rabbit

克隆类型Polyclonal

Raptor抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit,

产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量147kDa

细胞定位细胞浆

性    状Lyophilized or Liquid

浓    度1mg/1ml

免 疫 原KLH conjugated synthetic peptide derived from human Raptor

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

Raptor抗体产品介绍background:

mTOR controls cell growth, in part by regulating p70 S6 kinase alpha (p70alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70alpha. The binding of Raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha. Rapamycin or amino acid withdrawal increases, whereas insulin strongly inhibits, the recovery of 4EBP1 and raptor on 7-methyl-GTP Sepharose. Partial inhibition of raptor expression by RNA interference (RNAi) reduces mTOR-catalyzed 4EBP1 phosphorylation in vitro. RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo.


Function:

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.


Subunit:

Interacts with MTOR (By similarity). Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR and AKT1S1. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Binds directly to 4EBP1 and RPS6KB1 independently of its association with MTOR. Binds preferentially to poorly or non-phosphorylated forms of EIF4EBP1, and this binding is critical to the ability of MTOR to catalyze phosphorylation. Forms a complex with MTOR under both leucine-rich and -poor conditions. Interacts with ULK1 in a nutrient-dependent manner; the interaction is reduced during starvation. Interacts (when phosphorylated by AMPK) with 14-3-3 protein, leading to inhibit its activity.


Subcellular Location:

Cytoplasm. Lysosome. Note=Targeting to lysosomes depends on amino acid availability.


Tissue Specificity:

Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.


Post-translational modifications:

In response to nutrient limitation, phosphorylated by AMPK; phosphorylation promotes interaction with 14-3-3 proteins, leading to negative regulation of the mTORC1 complex. In response to growth factors, phosphorylated at Ser-719, Ser-721 and Ser-722 by RPS6KA1; phosphorylation positively stimulates mTORC1 activity.


Similarity:

Belongs to the WD repeat RAPTOR family.

Contains 7 WD repeats.


SWISS:

Q8N122


Gene ID:

57521

Raptor抗体antibody, Ab)是由效应B细胞(效应**B细胞)分泌,机体用于抵御外来物质,如病毒,**等抗原,结构呈“Y”字型的球状蛋白质,仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质,均被称作抗原,因此对于抗抗体(能够结合抗体的抗体)来说,抗体本身也是一种抗原物质。

   QQ图片20171030091318

Raptor抗体普通抗体重链和轻链的结构

重链结构:普通的**球蛋白具有2条重链(H链),分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型,对应的**球蛋白名称分别为IgMIgGIgAIgDIgE

轻链结构:  普通**球蛋白具有2条轻链(L链),分子质量约25kDa,有κ链和λ链两种亚型,这两种轻链决定了Ig的亚型类别(IgG1IgG2IgG3IgG4)。一个天然的Ig分子两条轻链总是相同的,但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同,正常人血清**球蛋白κ链:λ链约为21,而在小鼠的比例为201

2.2抗体Fab段和Fc

IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1Fc,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应;经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段(fragment of antigen bindingFab),相当于抗体分子的两个臂,由一个完整的轻链和重链的VHCH1结构域组成。Fc段为可结晶段(fragment crystallizableFc)相当于IgCH2CH3结构域,是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区,以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2CH3的区域,相当于Y字结构下面那一部分。

合格 IL36 gamma 白细胞介素36γ抗体
合格 TNNC2 肌钙蛋白C2抗体
合格 TNRC18 TNRC18蛋白抗体
合格 ERG/KCNH2 特异性钾离子通道蛋白抗体
合格 phospho-TOB (Ser164) 磷酸化HER2受体转换蛋白抗体
合格 合格 ISX/Intestine specific homeobox 胰肠同源结构域转录因子抗体
合格 INTS5 INTS5蛋白抗体
合格 Pro-neuregulin-1, membrane-bound isoform 神经胶质生长因子/神经调节蛋白β抗体
合格 IP3KC IP3KC蛋白抗体
合格 合格 IP6K1 肌醇六磷酸激酶1抗体
合格 LCHN LCHN蛋白抗体
合格 LCLAT1 心磷脂酰基转移酶1抗体
合格 LCN10 附睾特异蛋白10抗体
合格 合格 LCN8 附睾特异蛋白8抗体
合格 LCT 乳糖酶根皮苷水解酶1抗体
合格 合格 LCTL 乳糖酶样蛋白抗体
合格 LDLRAD2 低密度脂蛋白受体结构域蛋白2抗体
合格 LDLRAD3 低密度脂蛋白受体结构域蛋白3抗体
合格 Leishmania Major Surface Protease 利什曼原虫表面蛋白酶抗体
合格 LENG8 LENG8蛋白抗体
合格 LGALS9B 半乳糖凝集素9B抗体
合格 合格 LGI2 富含亮氨酸胶质瘤失活蛋白2抗体
合格 LHFPL1 脂肪瘤高迁移率融合蛋白样蛋白1抗体
合格 LHX8 LHX8蛋白抗体
合格 LIAS 硫辛酸合成酶抗体
合格 MYL2 心脏肌球蛋白轻链2抗体
 


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