Recombinant Human Endostatin
产品编号
产品名称
产品包装
产品价格
XY390-1mg
1mg
5665.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
80781
P39060
E. coli
177aa
19.8kDa
－
About this Protein
Name
Recombinant Human Endostatin (Recombinant Human Endostatin; rHuEndostatin)；重组人血管内皮抑制素
Synonyms
COL18A1; antiangiogenic agent; collagen alpha-1(XVIII) chain; collagen, type XVIII, alpha 1; FLJ27325; FLJ34914; human type XVIII collagen10endostatin; KNO; KNO1MGC74745; Knobloch syndrome, type 1; KS; multi-functional protein MFP
Purity
>96% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by inhibiting the FGF basic-dependent proliferation of HUVEC migration is less than 2.0μg/ml in the presence of AntiHuman Endostatin Polyclonal Antibody.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μM filtered concentrated solution in PBS, pH7.4.
Endotoxin
Less than 1EU/μg of rHuEndostatin as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Endostatin has been identified as a C-terminal fragment of Collagen type 18, a recently identified member of a family of collagen-like proteins referred to as multiplexin family. Endostatin specifically inhibits proliferation of endothelial cells although it does not affect the proliferation of EOMA cells. Endostatin also potently inhibits angiogenesis and tumor growth. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes. Recombinant Endosatin expressed in yeast causes G1 arrest of endothelial cells, and endostatin treatment results in apoptosis of HUVE and HMVE cells.
Amino Acid Sequence
NYEKPALHLA ALNMPFSGDI RADFQCFKQA RAAGLLSTYR AFLSSHLQDL STIVRKAERY SLPIVNLKGQ VLFNNWDSIF SGHGGQFNMH IPIYSFDGRD IMTDPSWPQK VIWHGSSPHG VRLVDNYCEA WRTADTAVTG LASPLSTGKI LDQKAYSCAN RLIVLCIENS FMTDARK
包装清单:
包装
XY390-20μg
20μg
XY390-100μg
100μg
-
说明书
1份
保存条件: -20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。 注意事项: 由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。 本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。 为了您的**和健康，请穿实验服并戴一次性手套操作。