LOCUS Exported 8535 bp ds-DNA circular SYN 11-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pKW08-Lx-Int
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8535)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sunday, September 11, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..8535
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(248..836)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
terminator 4797..4843
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 5617..5633
/note="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
terminator 8495..8522
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
ORIGIN
1 actagttcta gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
61 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
121 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
181 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
241 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
301 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
361 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
421 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
481 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
541 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
601 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
661 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
721 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
781 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
841 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
901 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
961 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
1021 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
1081 ccggacgtgg ccgaccagcc cgtcatcgtc aacgcctgac cgcggtgcgg acaggccgtg
1141 tcgcgaccgg ccgtgcggaa ttaagccggc ccgtaccctg tgaatagagg tccgctgtga
1201 cacaagaatc cctgttactt ctcgaccgta ttgattcgga tgattcctac gcgagcctgc
1261 ggaacgacca ggaattctgg gagccgctgg cccgccgagc cctggaggag ctcgggctgc
1321 cggtgccgcc ggtgctgcgg gtgcccggcg agagcaccaa ccccgtactg gtcggcgagc
1381 ccgacccggt gatcaagctg ttcggcgagc actggtgcgg tccggagagc ctcgcgtcgg
1441 agtcggaggc gtacgcggtc ctggcggacg ccccggtgcc ggtgccccgc ctcctcggcc
1501 gcggcgagct gcggcccggc accggagcct ggccgtggcc ctacctggtg atgagccgga
1561 tgaccggcac cacctggcgg tccgcgatgg acggcacgac cgaccggaac gcgctgctcg
1621 ccctggcccg cgaactcggc cgggtgctcg gccggctgca cagggtgccg ctgaccggga
1681 acaccgtgct caccccccat tccgaggtct tcccggaact gctgcgggaa cgccgcgcgg
1741 cgaccgtcga ggaccaccgc gggtggggct acctctcgcc ccggctgctg gaccgcctgg
1801 aggactggct gccggacgtg gacacgctgc tggccggccg cgaaccccgg ttcgtccacg
1861 gcgacctgca cgggaccaac atcttcgtgg acctggccgc gaccgaggtc accgggatcg
1921 tcgacttcac cgacgtctat gcgggagact cccgctacag cctggtgcaa ctgcatctca
1981 acgccttccg gggcgaccgc gagatcctgg ccgcgctgct cgacggggcg cagtggaagc
2041 ggaccgagga cttcgcccgc gaactgctcg ccttcacctt cctgcacgac ttcgaggtgt
2101 tcgaggagac cccgctggat ctctccggct tcaccgatcc ggaggaactg gcgcagttcc
2161 tctgggggcc gccggacacc gcccccggcg cctgacgccc cgggccgccc ggcgccgccc
2221 ccggcccccg gcggccgccc ggagccccgc ccgcgctcgg gagccccggg cccgcgccga
2281 agcccgctgc tgcgagcccg gagcgggccg gccgacggcg gtgcgggccc ggcggcggac
2341 gctcagcagc ggcgggcgtg aaaggccctg gcatcctcga tcatctcctc cagggtggtc
2401 ggccgagctt ccatcccagc tcggcaagga tcaacgagga cagtcgcacg acgaagttct
2461 tctggatcgc gcccgtgctg gaagcactca acctcgaagc gtgtggttgc ggagccatct
2521 agcaaccaca cgaaacatgc gcaacgaacc gcgcaacgaa caacgcctag aactggccct
2581 agatgagctg actcgtatcg ttggtaaacc tagtttgacc agcatgtttt aactacgttc
2641 ggtgagctgt caacggggcc tgtaacggca caacgaaccg tgcaacgaga gtggccacgg
2701 atgccaccac aggcactaca acggagttcg ccacgtacat caccacaacc accgattctg
2761 gcggtgagct ccccgatatt cagcggaaat ggcttggtat cgaccaagat tcgtagaacc
2821 ccgtctcgtc tggctggtat tcaaaacggg cgcaacgaaa cacgcaacga gacaggcatg
2881 gcccaaacca gaaaactagc gtctaccagg acttttacct gtccgacccg ttgcaacgga
2941 accccccacg gaacccccgc gacacccgct ccccaattgt tgctagctgg cgggaacggc
3001 aatgctcctg gcggcggcgg ggctggtgcc caggtctccg cacagagcgg cggtgctggc
3061 gctcgcggcc aggcgtggtt cttcgcgtac tgacaagaaa cccccctctt taggactcag
3121 tgtccttggg aggggggctt tttgcgtttc aggaggtctt ggccagcttg gacatcgcct
3181 cagcgatagc ctcgtcgcgg gcctcagacg ccatctggta cttcatcgcc atcctaggag
3241 tcgtgtgacc gagacgggcc atcagctcct tggtcgtcgc acctgcctga gcggcgaacg
3301 tagcgccgac agcgcggagg tcgtggatgc ggagttccgg ccgaccgatc ttggcgtagc
3361 cacgcttcag cgacttggtg aacgcggact tcgacagccg gttgccctgc gtcgtggtca
3421 ccaggaatgc ctcggggccc ttgttcatct tcgtacggtc cttcatgtgc gctcggatca
3481 tctccgcgac gtgaggcgga accgtcacag gacgcttcga ccggacggtc ttggcgttgc
3541 caacgacgat cttgttcccc acgcgggaag cgccacggcg cacccggagc ttcatcgtca
3601 tgccgtcgtc cacgatgtcc ttgcggcgaa gctcgatcag ctctccgaac cggaggctcg
3661 tccacgccag gatgtatgcc gcgatccggt agtgctcgaa gatctcagcg gcgacgatgt
3721 ccagctcctc aggcgtcagc gcctctacgt cgcgctcatc ggctgccttc tgctcgatcc
3781 ggcacgggtt ctctgcgatc agcttgtcct cgaccgctgt gttcatcacc gcccggagga
3841 cgttgtaggc atgccggcgg gcagtcgggt gcttcctacc catcccggcc caccacgcac
3901 gcaccagagc tggcgtcatc tctgtgaccg ccacttcacc tagcaccggg tagatgcggc
3961 gctccgcgtg cccgctgtac agatccctgg tgccgtctgc gaggtcgcgc tccacgagcc
4021 acttccgggt gtactcctcc agcgtgatgg cgctggcggc tgccttcttc gcccggtcct
4081 gtggaggggt ccaggtctcc atctcgatga gccgcttctc gcccgcgagc caggcttcgg
4141 cgtccatctt gttgtcgtag gtctgcagcg cgtagtacct cacaccgtcc tgcgggttga
4201 cgtatgaggc ttggatcctc ccgctgcgct gagtcttcag cgatccccat ccgcgacgtg
4261 ccaactaggt ctcctctcgt cgtgaacaag gctaccgggt tgcaactcct gtgcaactct
4321 caggcttcaa cgcgcttcta cgacctgcaa tttctttcca cttagaggat gcagccgaga
4381 gggggtaaaa acctatcttg accggcccat atatgtggtc ggcagacacc cattcttcca
4441 aactagctac gcgggttcga ttcccgtcgc ccgctccgct ggtcagaggg tgttttcgcc
4501 ctctggccat ttttctttcc aggggtctgc aactcttgtg cgactcttct gacctgggca
4561 tacgcggttg caacgcatcc ctgatctggc tactttcgat gctgacaaac gaatagagcc
4621 ccccgcctgc gcgaacagac gaggggcatt cacaccagat tggagctggt gcagtgaaga
4681 gaatagaccg ggacaaggtt gcaccgggag ttgcagcggt cggaaccctc gccgtcggcg
4741 ggctggcgtt cgccctgtcg ttcacggctc tagctgatca ccgcggccat gatggcataa
4801 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagtcgatc
4861 gtacaccggt gtatggtccg acacgagctc caagtcagat atttcgctga ggggccaccc
4921 cacaactgca cactcccccg ctctcccgtc gagccctggt ggtggaacac cagcgacagc
4981 cgagcacccc caaccacctg taccaaccag gaggaacaca tgcgtcgttt cgaggacgtt
5041 tccgggccgc tgagagccgc tgtggcggcc gtacacgccg ccttagaccc gttagacccc
5101 ctgccgcctg aatgcgcggg tacgagccac acagcgcccg aacttacgga gctggtgggc
5161 tcacctggct ttatggcgta cgaatcggct gtgtgcgacc tgttgggcga ggtgaggtac
5221 gcgctactca cgctggcaag ggcgacacag ccgccccacc gagcccgcac ggccgcgcgc
5281 ggtgtcaaca accgggtgag tcgtgcacac cagcaggtgt tcgaggcttg gctcgaagtg
5341 caggacatcg tggcgaacgc cgcccgatga gccgcgcctt acgctggctg ccagccgttc
5401 gcgggctggt tggtgcagcg cgtcgagcgg ttagaggccc tgcggtgttc caccaccgca
5461 ggcctcgccc tttttaaggc tgaatttgct tgtctccgaa tccaactggc ttgtccaagg
5521 gtgtatctac gcttaatcca aagttcaaac gaggggatta cacatgacca acttcgataa
5581 cgttctcggc tcgatgatcc ccggcgggcc ccccctcgag gtcgacggta tcgataaaat
5641 aaaaaagggg acctctaggg tccccaatta attagctaaa gcttttacga gtggtatttg
5701 acgatgttgg cgtttaccac atcgataacc gctctttgct gcgctttatc ttccatcgac
5761 tcaaaagaca tcaataggtc cgcggcacca caacactcaa tcgcaactcg cgctgcctga
5821 gtactttctt cataagtacc gatggcattt tctgacaaca gctctcccat tttttgctca
5881 aagtcggtat ttgagtaaga ttcacggaca aactcttcca aatacacgcg agcttctgcc
5941 cttgctgctt caccatctac attttggttg accagcagcg tcagcttgtg tcgaacctga
6001 ctaacatcga caccatgtgc ctgagcaact tcgtggtaca aaccggcgta ttcttttctt
6061 tgagcgtttg agtcgtccca tctaaacacg agtggaagcc ctaacttagc cgcccattca
6121 accacttctt tgctcgtcgc attcacaaat tgcgcaggac cgccttcagt gaacgcgtgt
6181 gggttaacgg agattttagg aaaactataa aaatcattgt ttggatggca gtacccagta
6241 gtgaatgaat cattgatgat cttgtgacac tcactgaaca actgaaactg agaatccgtt
6301 ggtcgattaa agaagcgcat atctgcactc ttttcacaat cactaaagcc aaaggcaaaa
6361 cggccttcac tcatttggtc aagtagacac gcttcttccg ccacacgtac tggatgatgc
6421 gtggtaatga cgtgattcaa cgaagccact ttggcgttct ttgtcatacc aagtaaaaaa
6481 ccagccactg ttagtggggc accaaccaca ccattgttcg agaaatggtt ttcgtaaaca
6541 gccaacgtgt caaacttcaa ctgatctacg taatgtgcgg tatctaacat ttcttcgatg
6601 acttgatcag aagaacgctt tgagttcata aaattgagga agaataatcc aaatttcatg
6661 tctctttcct tttagaaaat attaattact gtttttcttt gagatatggc atcacatcag
6721 actggaatag cttcatagat gcgataattt cttcttcaga accgtttgct tcaaaaccac
6781 aacaaatatt gtcaataccc gtcgcatcaa tatcttgctg gataatcgcg atacactctt
6841 caggcgtccc tactgggttg atttcgtagc tgtaatcaat tcggcgattg gtgtctttgt
6901 ggcctttcaa cacaaaatca cgccattgac ctttattgaa gtcgtaacct tttgtttggt
6961 cagagtcgtc aaaaatcttg gtggcattca cgtatgagtc gtaccaatgg cccaagaagt
7021 tgcggcaaat atctttggct ctatttgagt catgatcgac ggaggtgatg taagacaaac
7081 agtggtcaat cttagtcaca tcgtagccat gttcagtcgc gacttcgttg taaagatcaa
7141 gctgcgcttt cttctcgtga gtgttgatga tccagcttag aatcattggt aggccacgct
7201 ctgcagccca ttctgtcgtt gatgctgact ccgcgacgac ataaacagga gcaccacctt
7261 gtgtgtaagc cgatggattc agttggattt tcgggaactt aatatgttcg ttatccgccg
7321 cgatatagcc ttcattgaag ccttctttca tcaagtcata ccaacagtcc attaaggctc
7381 ggctgttatc catgtctgta ccaaagacac gaaaatcttt atcgtacaaa ccgcgacaaa
7441 taccaaaacg gaatcgtcct tttgacattt gatccagtag gtttacgtct tctgcttgtc
7501 gaaccggatg ggcagtcggc aatacgatag ctgcagtgcc aacgttgagc gtttctgtcg
7561 cacctaatag gtgtgcggca gcaacataag gattccctaa caacccaaat tcagtgaagt
7621 ggtgctctag caaccaaacg gtgtcgaagc cacaaccttc agacgctttg cccagattaa
7681 ccaatcgctt catcacttcg gtctgagata gctcaggtgg ctgataagtg aggaggaagt
7741 ttccaaattt cataacattt cctccggatc ctgtcaggat tccacgatga gagtagcgag
7801 cagtcagatg agagtagcga gcagtcactt tatctaagat aaagttatcg atgataaagt
7861 gcaactatga ctcaccaccg gaagcgtctc gaccgcacgt ccgtgttgct cggggcccgg
7921 caggtgctcg acgagactgg gctcgatggc ttcaccactc gcgcgctggc ggctcactta
7981 caggtacagc agcccggcct ctactggcac ttccgcacga aggccgaact cctcgcagcg
8041 ctggcgagcg acgtgcttga ccgagagcat cacgcttcct tccccgagtc cgacgagaaa
8101 tgggacgcat ttctcttgcg aaacgcaaga agtttccgcc acgcactaca cgcggtccgt
8161 gacggtgcgc gtctccacgc tgagcaccac cgcagaccat ccggcgacga caccgacgaa
8221 ggagctgagg cttccggtca gcaagtcggg cttctcgtgt ctcagggatt cgatgaacag
8281 acagccatca gcatgctcat cgcggtcagc cgatacaccg tcggtgtcgt actggaggag
8341 caaaccgcag aggtcggcaa tgcgagcgtt cctgaacgcg accgggaatt cgatttcggc
8401 ctgacagcac tgatcgacgg attctctcac tcccgcacag cgtcattgac ctcgagcgaa
8461 tcgtgatcta gaggtttaaa cagtattaaa cgcaaaaagg ccatccgtca ggatggcctt
8521 ctgcttagct aatca
//