LOCUS Exported 6089 bp ds-DNA circular SYN 30-AUG-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAN8-1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6089)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..6089
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 1..2129
/note="gpdA Promoter"
misc_feature 2178..2293
/note="Intron"
CDS 2302..2676
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="Zeo"
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
misc_feature complement(2733..3456)
/note="trpC terminator"
primer_bind complement(3464..3480)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 3954..4058
/gene="bla"
/note="AmpR promoter"
CDS 4059..4919
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5090..5678
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 5966..5987
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6002..6032
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 6040..6056
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6064..6080
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
61 cgtttatatc ttgcatcgtc ccaaagctat tggcgggata ttctgtttgc agttggctga
121 cttgaagtaa tctctgcaga tctttcgaca ctgaaatacg tcgagcctgc tccgcttgga
181 agcggcgagg agcctcgtcc tgtcacaact accaacatgg agtacgataa gggccagttc
241 cgccagctca ttaagagcca gttcatgggc gttggcatga tggccgtcat gcatctgtac
301 ttcaagtaca ccaacgctct tctgatccag tcgatcatcc gctgaaggcg ctttcgaatc
361 tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
421 ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
481 ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
541 cggagcggtg tcaagaggat attcttcgac tctgtattat agataagatg atgaggaatt
601 ggaggtagca tagcttcatt tggatttgct ttccaggctg agactctagc ttggagcata
661 gagggtcctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tccctgtgaa
721 ccttttattc accaatgagc attggaatga acatgaatct gaggactgca atcgccatga
781 ggttttcgaa atacatccgg atgtcgaagg cttggggcac ctgcgttggt tgaatttaga
841 acgtggcact attgatcatc cgatagctct gcaaagggcg ttgcacaatg caagtcaaac
901 gttgctagca gttccaggtg gaatgttatg atgagcattg tattaaatca ggagatatag
961 catgatctct agttagctca ccacaaaagt cagacggcgt aaccaaaagt cacacaacac
1021 aagctgtaag gatttcggca cggctacgga agacggagaa gccaccttca gtggactcga
1081 gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
1141 aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
1201 ataaacttta agcctaaact atacagaata agataggtgg agagcttata ccgagctccc
1261 aaatctgtcc agatcatggt tgaccggtgc ctggatcttc ctatagaatc atccttattc
1321 gttgacctag ctgattctgg agtgacccag agggtcatga cttgagccta aaatccgccg
1381 cctccaccat ttgtagaaaa atgtgacgaa ctcgtgagct ctgtacagtg accggtgact
1441 ctttctggca tgcggagaga cggacggacg cagagagaag ggctgagtaa taagccactg
1501 gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg gaccggccgc
1561 ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat ctattgcatc
1621 atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa tgtgaagcca
1681 ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa gtccaattgc
1741 ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta gagcgagtac
1801 ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca aatatcgtgc
1861 ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg gccagcggcg
1921 cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg acctgctgag
1981 gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg tcggcggggt
2041 tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc tccccaccag
2101 ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc ccatccaaga
2161 acctttattt cccctaagta agtactttgc tacatccata ctccatcctt cccatccctt
2221 attcctttga acctttcagt tcgagctttc ccacttcatc gcagcttgac taacagctac
2281 cccgcttgag cagacatcac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
2341 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
2401 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
2461 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
2521 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
2581 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
2641 aactgcgtgc acttcgtggc cgaggagcag gactgaccga cgccgaccaa caccgccggt
2701 ccgacggcgg cccacgggtc ccaggagctt gagatccact taacgttact gaaatcatca
2761 aacagcttga cgaatctgga tataagatcg ttggtgtcga tgtcagctcc ggagttgaga
2821 caaatggtgt tcaggatctc gataagatac gttcatttgt ccaagcagca aagagtgcct
2881 tctagtgatt taatagctcc atgtcaacaa gaataaaacg cgttttcggg tttacctctt
2941 ccagatacag ctcatctgca atgcattaat gcattgactg caacctagta acgccttnca
3001 ggctccggcg aagagaagaa tagcttagca gagctatttt cattttcggg agacgagatc
3061 aagcagatca acggtcgtca agagacctac gagactgagg aatccgctct tggctccacg
3121 cgactatata tttgtctcta attgtacttt gacatgctcc tcttctttac tctgatagct
3181 tgactatgaa aattccgtca ccagcncctg ggttcgcaaa gataattgca tgtttcttcc
3241 ttgaactctc aagcctacag gacacacatt catcgtaggt ataaacctcg aaatcanttc
3301 ctactaagat ggtatacaat agtaaccatg catggttgcc tagtgaatgc tccgtaacac
3361 ccaatacgcc ggccgaaact tttttacaac tctcctatga gtcgtttacc cagaatgcac
3421 aggtacactt gtttagaggt aatccttctt tctagagctt ggcactggcc gtcgttttac
3481 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
3541 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
3601 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
3661 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
3721 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
3781 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
3841 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
3901 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
3961 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
4021 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
4081 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
4141 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
4201 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
4261 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
4321 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
4381 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
4441 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
4501 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
4561 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
4621 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
4681 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
4741 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
4801 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
4861 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
4921 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
4981 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
5041 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
5101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
5161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
5221 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
5281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
5341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
5401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
5461 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
5521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
5581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
5641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
5701 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
5761 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
5821 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
5881 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
5941 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
6001 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
6061 acacaggaaa cagctatgac catgattac
//