LOCUS Exported 3410 bp ds-DNA circular SYN 04-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pPD49.26-L754
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3410)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sunday, September 4, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..3410
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1289..1393
/gene="bla"
/note="AmpR promoter"
CDS 1394..2254
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2425..3013
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 3301..3322
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3337..3367
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 3375..3391
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 3399..5
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggat
61 tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
121 gaggaccctt ggctagcgtc gacggtacca tggtattgat atctgagctc cgcatcggcc
181 gctgtcatca gatcgccatc tcgcgcccgt gcctctgact tctaagtcca attactcttc
241 aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa
301 aacttctctt aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg
361 tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc
421 ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt
481 tttttacttc tgataaattt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt
541 atcatatgtt acgtttcagt ttatgaccgc aatttttatt tcttcgcacg tctgggcctc
601 tcatgacgtc aaatcatgct catcgtgaaa aagttttgga gtatttttgg aatttttcaa
661 tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct ttttggggtt tcccctattg
721 tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg
781 atgcaagaaa gatcggaaga aggtttgggt ttgaggctca gtggaaggtg agtagaagtt
841 gataatttga aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc
901 ccaatatacc aaacataact gtttcctact agtcggccgt acgggccctt tcgtctcgcg
961 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
1021 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
1081 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
1141 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcggccttaa
1201 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
1261 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
1321 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
1381 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
1441 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
1501 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
1561 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
1621 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
1681 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
1741 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
1801 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
1861 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
1921 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
1981 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
2041 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
2101 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
2161 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
2221 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
2281 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
2341 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
2401 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
2461 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
2521 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
2581 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
2641 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
2701 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
2761 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg
2821 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
2881 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
2941 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
3001 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
3061 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
3121 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
3181 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
3241 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
3301 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
3361 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
//