LOCUS Exported 6118 bp ds-DNA circular SYN 25-AUG-2017
DEFINITION synthetic circular DNA
KEYWORDS pAC5.1b-EGFP②
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6118)
TITLE Direct Submission
JOURNAL Exported Aug 25, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..6118
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 56..2568
/label=Ac5 promoter
/note="Drosophila melanogaster actin 5C promoter"
primer_bind 2525..2544
/label=AC5-F
CDS 2617..3333
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
misc_feature 3336..3402
/label=MCS
CDS 3403..3444
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS 3454..3471
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
polyA_signal 3544..3678
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3622..3641)
/label=Sv40-polyA-R
rep_origin complement(4300..4888)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5059..5919)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5920..6024)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
2581 tacctactag tccagtgtgg tggaatttcg agctttatgg tgagcaaggg cgaggagctg
2641 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
2701 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
2761 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
2821 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
2881 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
2941 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
3001 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
3061 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
3121 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
3181 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
3241 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
3301 gggatcactc tcggcatgga cgagctgtac aagccaagct tgaattctgc agatatccag
3361 cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac
3421 cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa
3481 acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc agcctcgatc
3541 gaggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
3601 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
3661 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
3721 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
3781 gatcagtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
3841 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
3901 ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat
3961 gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac
4021 tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga
4081 cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat
4141 gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca
4201 ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta ccagccagca
4261 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
4321 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
4381 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
4441 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
4501 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
4561 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
4621 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
4681 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
4741 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
4801 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
4861 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
4921 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
4981 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
5041 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
5101 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
5161 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
5221 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
5281 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
5341 agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc
5401 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
5461 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
5521 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
5581 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
5641 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
5701 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
5761 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
5821 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
5881 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
5941 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
6001 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
6061 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgt
//