LOCUS Exported 6535 bp ds-DNA circular SYN 26-NOV-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6535)
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..6535
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 398..432
/label=xylA Promoter
misc_feature 500..512
/label=RBS
misc_feature 520..608
/label=MCS
rep_origin complement(741..1329)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1500..2360)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2361..2465)
/gene="bla"
/label=AmpR promoter
CDS complement(2629..4005)
/codon_start=1
/gene="Bacillus subtilis tetL"
/product="tetracycline efflux protein"
/label=TcR
/note="confers resistance to tetracycline"
/translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
"
CDS complement(4205..5209)
/codon_start=1
/gene="repB"
/product="RepB replication protein"
/label=repB
/note="from Enterococcus faecalis plasmid pAM-alpha-1"
/db_xref="GI:22652809"
/protein_id="AAN03827.1"
/translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
CDS complement(5663..252)
/codon_start=1
/label=xylR
/translation="MNQKLILDEILKNSPVSRATLSEITGLNKSTVSSQVNTLLEKDFI
FEIGAGQSRGGRRPVMLVFNKNAGYSIGIDIGVDYLNGILTDLEGNIILEKTSDLSSSS
ASEVKEILFALIHGFVTHMPESPYGLVGIGICVPGLVDRHQQIIFMPNLNWNIKDLQFL
IESEFNVPVFVENEANAGAYGEKVFGMTKNYENIVYISINIGIGTGLVINNELYKGVQG
FSGEMGHMTIDFNGPKCSCGNRGCWELYASEKALLASLSKEEKNISRKEIVERANKNDV
EMLNALQNFGFYIGIGLTNILNTFDIEAVILRNHIIESHPIVLNTIKNEVSSRVHSHLD
NKCELLPSSLGKNAPALGAVSIVIDSFLSVTPIS"
ORIGIN
1 aattccgtta agatagtcga ctcctatatc aataccaatc gagtagcctg cattcttatt
61 aaaaacaagc attacaggtc ttctgccgcc tctagattgc cctgccccaa tttcaaaaat
121 aaaatctttt tcaagcagtg tatttacttg agaggagaca gtagacttgt ttaatcctgt
181 aatctcagag agagttgccc tggagacagg ggagttcttc aaaatttcat ctaatattaa
241 tttttgattc atttttttta ctaaagcttg atctgcaatt tgaataataa ccactccttt
301 gtttatccac cgaactaagt tggtgttttt tgaagcttga attagatatt taaaagtatc
361 atatctaata ttataactaa attttctaaa aaaaacattg aaataaacat ttattttgta
421 tatgatgaga taaagttagt ttattggata aacaaactaa ctcaattaag atagttgatg
481 gataaacttg ttcacttaaa tcaaaggggg aaatgtacaa tggtccaaac tagttcgaag
541 atctccggag ctcccgggat ccggtaccgg ccgcatgccg gctaaacctc gcgaacggat
601 tcaccggtcc aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca
661 acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct
721 cgggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
781 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
841 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
901 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
961 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
1021 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
1081 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
1141 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
1201 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
1261 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
1321 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
1381 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
1441 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
1501 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
1561 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
1621 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
1681 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
1741 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
1801 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
1861 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
1921 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
1981 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
2041 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
2101 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
2161 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
2221 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
2281 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
2341 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
2401 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
2461 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
2521 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt cctgttataa
2581 aaaaaggatc aattttgaac tctctcccaa agttgatccc ttaacgattt agaaatccct
2641 ttgagaatgt ttatatacat tcaaggtaac cagccaacta atgacaatga ttcctgaaaa
2701 aagtaataac aaattactat acagataagt tgactgatca acttccatag gtaacaacct
2761 ttgatcaagt aagggtatgg ataataaacc acctacaatt gcaatacctg ttccctctga
2821 taaaaagctg gtaaagttaa gcaaactcat tccagcacca gcttcctgct gtttcaagct
2881 acttgaaaca attgttgata taactgtttt ggtgaacgaa agcccaccta aaacaaatac
2941 gattataatt gtcatgaacc atgatgttgt ttctaaaaga aaggaagcag ttaaaaagct
3001 aacagaaaga aatgtaactc cgatgtttaa cacgtataaa ggacctcttc tatcaacaag
3061 tatcccacca atgtagccga aaataatgac actcattgtt ccagggaaaa taattacact
3121 tccgatttcg gcagtactta gctggtgaac atctttcatc atataaggaa ccatagagac
3181 aaaccctgct actgttccaa atataattcc cccacaaaga actccaatca taaaaggtat
3241 atttttccct aatccgggat caacaaaagg atctgttact ttcctgatat gttttacaaa
3301 tatcaggaat gacagcacgc taacgataag aaaagaaatg ctatatgatg ttgtaaacaa
3361 cataaaaaat acaatgccta cagacattag tataattcct ttgatatcaa aatgaccttt
3421 tatccttact tctttcttta ataatttcat aagaaacgga acagtgataa ttgttatcat
3481 aggaatgagt agaagatagg accaatgaat ataatgggct atcattccac caatcgctgg
3541 accgactcct tctcccatgg ctactatcga tccaataaga ccaaatgctt tacccctatt
3601 ttcctttgga atatagcgcg caactacaac cattacgagt gctggaaatg cagctgcacc
3661 agccccttga ataaaacgag ccataataag taaggaaaag aaagaatggc caacaaaccc
3721 aattaccgac ccgaaacaat ttattataat tccaaatagg agtaaccttt tgatgcctaa
3781 ttgatcagat agctttccat atacagctgt tccaatggaa aaggttaaca taaaggctgt
3841 gttcacccag tttgtactcg caggtggttt attaaaatca tttgcaatat caggtaatga
3901 gacgttcaaa accatttcat ttaatacgct aaaaaaagat aaaatgcaaa gccaaattaa
3961 aatttggttg tgtcgtaaat tcgattgtga ataggatgta ttcacatttc accctccaat
4021 aatgagggca gacgtagttt atagggttaa tgatacgctt ccctctttta attgaaccct
4081 gttacattca ttacacttca taattaattc ctcctaaact tgattaaaac attttaccac
4141 atataaacta agttttaaat tcagtatttc atcacttata caacaatatg gcccgtttgt
4201 tgaactactc tttaataaaa taatttttcc gttcccaatt ccacattgca ataatagaaa
4261 atccatcttc atcggctttt tcgtcatcat ctgtatgaat caaatcgcct tcttctgtgt
4321 catcaaggtt taatttttta tgtatttctt ttaacaaacc accataggag attaaccttt
4381 tacggtgtaa accttcctcc aaatcagaca aacgtttcaa attcttttct tcatcatcgg
4441 tcataaaatc cgtatccttt acaggatatt ttgcagtttc gtcaattgcc gattgtatat
4501 ccgatttata tttatttttc ggtcgaatca tttgaacttt tacatttgga tcatagtcta
4561 atttcattgc ctttttccaa aattgaatcc attgtttttg attcacgtag ttttctgtat
4621 tcttaaaata agttggttcc acacatacca atacatgcat gtgctgatta taagaattat
4681 ctttattatt tattgtcact tccgttgcac gcataaaacc aacaagattt ttattaattt
4741 ttttatattg catcattcgg cgaaatcctt gagccatatc tgacaaactc ttatttaatt
4801 cttcgccatc ataaacattt ttaactgtta atgtgagaaa caaccaacga actgttggct
4861 tttgtttaat aacttcagca acaacctttt gtgactgaat gccatgtttc attgctctcc
4921 tccagttgca cattggacaa agcctggatt tacaaaacca cactcgatac aactttcttt
4981 cgcctgtttc acgattttgt ttatactcta atatttcagc acaatctttt actctttcag
5041 cctttttaaa ttcaagaata tgcagaagtt caaagtaatc aacattagcg attttctttt
5101 ctctccatgg tctcactttt ccactttttg tcttgtccac taaaaccctt gatttttcat
5161 ctgaataaat gctactatta ggacacataa tattaaaaga aacccccatc tatttagtta
5221 tttgtttggt cacttataac tttaacagat ggggtttttc tgtgcaacca attttaaggg
5281 ttttcaatac tttaaaacac atacatacca acacttcaac gcacctttca gcaactaaaa
5341 taaaaatgac gttatttcta tatgtatcaa gataagaaag aacaagttca aaaccatcaa
5401 aaaaagacac cttttcaggt gcttttttta ttttataaac tcattccctg atctcgactt
5461 cgttcttttt ttacctctcg gttatgagtt agttcaaatt cgttcttttt aggttctaaa
5521 tcgtgttttt cttggaattg tgctgtttta tcctttacct tgtctacaaa ccccttaaaa
5581 acgtttttaa aggcttttaa gccgtctgta cgttccttaa gatcaacgtg atataggttt
5641 gctaaccttt gcgttcactt aactaactta taggggtaac acttaaaaaa gaatcaataa
5701 cgatagaaac cgctcctaaa gcaggtgcat tttttcctaa cgaagaaggc aatagttcac
5761 atttattgtc taaatgagaa tggactctag aagaaacttc gtttttaatc gtatttaaaa
5821 caatgggatg agattcaatt atatgatttc tcaagataac agcttctata tcaaatgtat
5881 taaggatatt ggttaatcca attccgatat aaaagccaaa gttttgaagt gcatttaaca
5941 tttctacatc atttttattt gcgcgttcca caatctcttt tcgagaaata ttcttttctt
6001 ctttagagag cgaagccagt aacgcttttt cagaagcata taattcccaa cagcctcgat
6061 ttccacagct gcatttgggt ccattaaaat ctatcgtcat atgacccatt tccccagaaa
6121 aaccctgaac acctttatac aattcgttgt taataacaag tccagttcca attccgatat
6181 taatactgat gtaaacgatg ttttcatagt tttttgtcat accaaatact ttttcaccgt
6241 atgctcctgc attagcttca ttttcaacaa aaaccggaac attaaactca ctctcaatta
6301 aaaactgcaa atctttgata ttccaattta agttaggcat gaaaataatt tgctgatgac
6361 gatctacaag gcctggaaca caaattccta ttccgactag accataaggg gactcaggca
6421 tatgggttac aaaaccatga ataagtgcaa ataaaatctc ttttacttca ctagcggaag
6481 aactagacaa gtcagaagtc ttctcgagaa taatatttcc ttctaagtcg gttag
//