LOCUS Exported 5524 bp ds-DNA circular SYN 10-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 20
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5524)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Saturday, September 10, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..5524
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 5..406
/gene="S. cerevisiae ADH1"
/note="ADH1 promoter"
/note="promoter for alcohol dehydrogenase 1"
CDS 434..874
/codon_start=1
/gene="Saccharomyces cerevisiae GAL4 (truncated)"
/product="DNA binding domain of the GAL4 transcriptional
activator"
/note="GAL4 DNA binding domain"
/translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
terminator 921..1108
/gene="S. cerevisiae ADH1"
/note="ADH1 terminator"
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
CDS complement(1161..1835)
/codon_start=1
/gene="S. cerevisiae TRP1"
/product="phosphoribosylanthranilate isomerase, required
for tryptophan biosynthesis"
/note="TRP1"
/note="yeast auxotrophic marker"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
KK"
promoter complement(1836..1937)
/gene="S. cerevisiae TRP1"
/note="TRP1 promoter"
primer_bind complement(1952..1968)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 1976..1992
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2000..2030)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
rep_origin complement(2354..2942)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3113..3973)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3974..4078)
/gene="bla"
/note="AmpR promoter"
rep_origin 4360..5524
/note="2u ori"
/note="yeast 2u plasmid origin of replication"
ORIGIN
1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gcttgaagca
421 agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact
481 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
541 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
601 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
661 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt
721 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac
781 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag
841 tagtaacaaa ggtcaaagac agttgactgt atcgccggaa ttcccgggga tccgtcgacc
901 tgcagccaag ctaattccgg gcgaatttct tatgatttat gatttttatt attaaataag
961 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
1021 tcttgttctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg
1081 tcgctcttat tgaccacacc tctaccggca tgccggcaag tgcacaaaca atacttaaat
1141 aaatactact cagtaataac ctatttctta gcatttttga cgaaatttgc tattttgtta
1201 gagtctttta caccatttgt ctccacacct ccgcttacat caacaccaat aacgccattt
1261 aatctaagcg catcaccaac attttctggc gtcagtccac cagctaacat aaaatgtaag
1321 ctttcggggc tctcttgcct tccaacccag tcagaaatcg agttccaatc caaaagttca
1381 cctgtcccac ctgcttctga atcaaacaag ggaataaacg aatgaggttt ctgtgaagct
1441 gcactgagta gtatgttgca gtcttttgga aatacgagtc ttttaataac tggcaaaccg
1501 aggaactctt ggtattcttg ccacgactca tctccatgca gttggacgat atcaatgccg
1561 taatcattga ccagagccaa aacatcctcc ttaggttgat tacgaaacac gccaaccaag
1621 tatttcggag tgcctgaact atttttatat gcttttacaa gacttgaaat tttccttgca
1681 ataaccgggt caattgttct ctttctattg ggcacacata taatacccag caagtcagca
1741 tcggaatcta gagcacattc tgcggcctct gtgctctgca agccgcaaac tttcaccaat
1801 ggaccagaac tacctgtgaa attaataaca gacatactcc aagctgcctt tgtgtgctta
1861 atcacgtata ctcacgtgct caatagtcac caatgccctc cctcttggcc ctctcctttt
1921 cttttttcga ccgaattaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
1981 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
2041 cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
2101 gaaacctgtc gtgccagctg gattaatgaa tcggccaacg cgcggggaga ggcggtttgc
2161 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
2221 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
2281 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
2341 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
2401 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
2461 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
2521 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
2581 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
2641 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
2701 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
2761 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
2821 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
2881 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
2941 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
3001 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
3061 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
3121 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
3181 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
3241 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
3301 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
3361 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
3421 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
3481 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
3541 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
3601 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
3661 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
3721 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
3781 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
3841 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
3901 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
3961 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
4021 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
4081 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
4141 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
4201 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
4261 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
4321 atgcggcatc agagcagatt gtactgagag tgcaccataa cgcatttaag cataaacacg
4381 cactatgccg ttcttctcat gtatatatat atacaggcaa cacgcagata taggtgcgac
4441 gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt tcggaagcgc tcgttttcgg
4501 aaacgctttg aagttcctat tccgaagttc ctattctcta gctagaaagt ataggaactt
4561 cagagcgctt ttgaaaacca aaagcgctct gaagacgcac tttcaaaaaa ccaaaaacgc
4621 accggactgt aacgagctac taaaatattg cgaataccgc ttccacaaac attgctcaaa
4681 agtatctctt tgctatatat ctctgtgcta tatccctata taacctaccc atccaccttt
4741 cgctccttga acttgcatct aaactcgacc tctacatttt ttatgtttat ctctagtatt
4801 actctttaga caaaaaaatt gtagtaagaa ctattcatag agtgaatcga aaacaatacg
4861 aaaatgtaaa catttcctat acgtagtata tagagacaaa atagaagaaa ccgttcataa
4921 ttttctgacc aatgaagaat catcaacgct atcactttct gttcacaaag tatgcgcaat
4981 ccacatcggt atagaatata atcggggatg cctttatctt gaaaaaatgc acccgcagct
5041 tcgctagtaa tcagtaaacg cgggaagtgg agtcaggctt tttttatgga agagaaaata
5101 gacaccaaag tagccttctt ctaaccttaa cggacctaca gtgcaaaaag ttatcaagag
5161 actgcattat agagcgcaca aaggagaaaa aaagtaatct aagatgcttt gttagaaaaa
5221 tagcgctctc gggatgcatt tttgtagaac aaaaaagaag tatagattct ttgttggtaa
5281 aatagcgctc tcgcgttgca tttctgttct gtaaaaatgc agctcagatt ctttgtttga
5341 aaaattagcg ctctcgcgtt gcatttttgt tttacaaaaa tgaagcacag attcttcgtt
5401 ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa aatgcagctc agattctttg
5461 tttgaaaaat tagcgctctc gcgttgcatt tttgttctac aaaatgaagc acagatgctt
5521 cgtt
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