LOCUS Exported 6867 bp ds-DNA circular SYN 06-JAN-2018
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..6867
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 185..3322
/codon_start=1
/label=LacZI
/translation="MTGSGAWLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSR
LNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEAD
TVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRI
IFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQ
DMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVS
LWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTA
DGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDIL
LMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAM
SERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTA
TDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQ
AFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTP
HPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDV
APQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLP
AASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLD
NDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTL
FISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPD
RLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQL
METSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK"
rep_origin complement(4019..4607)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4778..5638)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5639..5743)
/gene="bla"
/label=AmpR promoter
CDS complement(5848..6651)
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
promoter complement(6652..6867)
/gene="S. cerevisiae URA3"
/label=URA3 promoter
ORIGIN
1 aagcttgaat tcgagctcgg tacccgggga tctgtcgacc tcgaggcatg tgctctgtat
61 gtatataaaa ctcttgtttt cttcttttct ctaaatattc tttccttata cattaggtcc
121 tttgtagcat aaattactat acttctatag acacgcaaac acaaatacac acactaaatt
181 aataatgacc ggatccggag cttggctgtt gcccgtctca ctggtgaaaa gaaaaaccac
241 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
301 ggcacgacag gtttcccgac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
361 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
421 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga
481 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc
541 gcccatctac accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga
601 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg
661 ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg
721 ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt
781 acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct
841 ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa
901 accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg
961 cgctgtactg gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac
1021 agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga
1081 aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa
1141 cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac
1201 cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat
1261 tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca
1321 cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct
1381 gctgatgaag cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct
1441 gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac
1501 ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag
1561 cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc
1621 gctggggaat gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc
1681 tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga
1741 tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa
1801 atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga
1861 atacgcccac gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg
1921 tcagtatccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa
1981 atatgatgaa aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa
2041 cgatcgccag ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct
2101 gacggaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga
2161 agtgaccagc gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc
2221 gctggatggt aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa
2281 acagttgatt gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac
2341 agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg
2401 gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat
2461 cccgcatctg accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca
2521 atttaaccgc cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct
2581 gacgccgctg cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga
2641 agcgacccgc attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca
2701 ggccgaagca gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac
2761 gaccgctcac gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg
2821 gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc
2881 gcatccggcg cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg
2941 gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg
3001 ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct
3061 gcgctgcggg acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt
3121 caacatcagc cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca
3181 cgcggaagaa ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga
3241 ctcctggagc ccgtcagtat cggcggaatt acagctgagc gccggtcgct accattacca
3301 gttggtctgg tgtcaaaaat aataataacc gggcaggcca tgtctgcccg tatttcgcgt
3361 aaggaaatcc attatgtact atttaaaaaa cacaaacttt tggatgttcg gtttattctt
3421 tttcttttac ttttttatca tgggagccta cttcccgttt ttcccgattt ggctacatga
3481 catcaaccat atcagcaaaa gtgatacggg tattattttt gccgctattt ctctgttctc
3541 gctattattc caaccgctgt ttggtctgct ttctgacaaa ctcggcctcg actctagcga
3601 attaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
3661 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
3721 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
3781 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
3841 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
3901 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
3961 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
4021 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
4081 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
4141 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
4201 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
4261 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
4321 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
4381 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
4441 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
4501 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
4561 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
4621 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
4681 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
4741 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
4801 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
4861 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
4921 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
4981 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
5041 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
5101 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
5161 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
5221 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
5281 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
5341 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
5401 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
5461 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
5521 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
5581 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
5641 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
5701 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
5761 tgccacctgg gtaataactc gatataatta aattgaagct ctaatttgtg agtttagtat
5821 acatgcattt acttataata cagtttttta gttttgctgg ccgcatcttc tcaaatatgc
5881 ttcccagcct gcttttctgt aacgttcacc ctctacctta gcatcccttc cctttgcaaa
5941 tagtcctctt ccaacaataa taatgtcaga tcctgtagag accacatcat ccacggttct
6001 atactgttga cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg tcataatcaa
6061 ccaatcgtaa ccttcatctc ttccacccat gtctctttga gcaataaagc cgataacaaa
6121 atctttgtcg ctcttcgcaa tgtcaacagt acccttagta tattctccag tagataggga
6181 gcccttgcat gacaattctg ctaacatcaa aaggcctcta ggttcctttg ttacttcttc
6241 tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg cattcgtaat
6301 gtctgcccat tctgctattc tgtatacacc cgcagagtac tgcaatttga ctgtattacc
6361 aatgtcagca aattttctgt cttcgaagag taaaaaattg tacttggcgg ataatgcctt
6421 tagcggctta actgtgccct ccatggaaaa atcagtcaag atatccacat gtgtttttag
6481 taaacaaatt ttgggaccta atgcttcaac taactccagt aattccttgg tggtacgaac
6541 atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg atattaaata gcttggcagc
6601 aacaggacta ggatgagtag cagcacgttc cttatatgta gctttcgaca tgatttatct
6661 tcgtttcctg caggtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt
6721 ttcttcaaca ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc
6781 ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa ggaaaccgaa atcaaaaaaa
6841 agaataaaaa aaaaatgatg aattgaa
//