LOCUS Exported 4967 bp ds-DNA circular SYN 12-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4967)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Monday, September 12, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4967
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 315..502
/gene="S. cerevisiae HIS3"
/note="HIS3 promoter"
CDS 503..1162
/codon_start=1
/gene="S. cerevisiae HIS3"
/product="imidazoleglycerol-phosphate dehydratase, required
for histidine biosynthesis"
/note="HIS3"
/note="yeast auxotrophic marker"
/translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
rep_origin complement(1423..1878)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(1661..2236)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITPSSELTLTKGNKSWVPGPPSRSTVSISLISNSCSPGDPLV
LERPPPRWSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAG
FPRQALNRGLPLGFRFSALRHLDPKKLD"
primer_bind 2023..2039
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 2046..2064
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 2073..2180
/note="MCS"
/note="pBluescript multiple cloning site"
primer_bind 2097..2113
/note="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2147..2163)
/note="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(2193..2211)
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(2232..2248)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 2256..2272
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2280..2310)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
rep_origin complement(2634..3222)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3393..4253)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4254..4358)
/gene="bla"
/note="AmpR promoter"
misc_feature 4395..4898
/note="CEN/ARS"
/note="S. cerevisiae CEN6 centromere fused to an
autonomously replicating sequence"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accataattc cgttttaaga gcttggtgag cgctaggagt cactgccagg tatcgtttga
241 acacggcatt agtcagggaa gtcataacac agtcctttcc cgcaattttc tttttctatt
301 actcttggcc tcctctagta cactctatat ttttttatgc ctcggtaatg attttcattt
361 ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat tatcacataa
421 tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa aaaatgagca
481 ggcaagataa acgaaggcaa agatgacaga gcagaaagcc ctagtaaagc gtattacaaa
541 tgaaaccaag attcagattg cgatctcttt aaagggtggt cccctagcga tagagcactc
601 gatcttccca gaaaaagagg cagaagcagt agcagaacag gccacacaat cgcaagtgat
661 taacgtccac acaggtatag ggtttctgga ccatatgata catgctctgg ccaagcattc
721 cggctggtcg ctaatcgttg agtgcattgg tgacttacac atagacgacc atcacaccac
781 tgaagactgc gggattgctc tcggtcaagc ttttaaagag gccctactgg cgcgtggagt
841 aaaaaggttt ggatcaggat ttgcgccttt ggatgaggca ctttccagag cggtggtaga
901 tctttcgaac aggccgtacg cagttgtcga acttggtttg caaagggaga aagtaggaga
961 tctctcttgc gagatgatcc cgcattttct tgaaagcttt gcagaggcta gcagaattac
1021 cctccacgtt gattgtctgc gaggcaagaa tgatcatcac cgtagtgaga gtgcgttcaa
1081 ggctcttgcg gttgccataa gagaagccac ctcgcccaat ggtaccaacg atgttccctc
1141 caccaaaggt gttcttatgt agtgacaccg attatttaaa gctgcagcat acgatatata
1201 tacatgtgta tatatgtata cctatgaatg tcagtaagta tgtatacgaa cagtatgata
1261 ctgaagatga caaggtaatg catcattcta tacgtgtcat tctgaacgag gcgcgctttc
1321 cttttttctt tttgcttttt cttttttttt ctcttgaact cgacggatca tatgcggtgt
1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata
1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa ggggggatgt gctgcaaggc
1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
2041 aattgtaata cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta
2101 gaactagtgg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc
2161 tcgagggggg gcccggtacc cagcttttgt tccctttagt gagggttaat tccgagcttg
2221 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
2281 aacataggag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc
2341 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
2401 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
2461 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
2521 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
2581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
2641 aggctcggcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
2701 ccgacaggac tataaagata ccaggcgttc ccccctggaa gctccctcgt gcgctctcct
2761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
2821 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
2881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
2941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
3001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
3061 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
3121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
3181 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
3241 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
3301 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
3361 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
3421 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactgcccgt cgtgtagata
3481 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
3541 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
3601 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
3661 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
3721 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
3781 gttacatgat cccccatgtt gtgaaaaaaa gcggttagct ccttcggtcc tccgatcgtt
3841 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
3901 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
3961 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
4021 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
4081 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
4141 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
4201 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
4261 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
4321 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
4381 cctgggtcct tttcatcacg tgctataaaa ataattataa tttaaatttt ttaatataaa
4441 tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc
4501 cgaagatgta aaagactcta gggggatcgc caacaaatac taccttttat cttgctcttc
4561 ctgctctcag gtattaatgc cgaattgttt catcttgtct gtgtagaaga ccacacacga
4621 aaatcctgtg attttacatt ttacttatcg ttaatcgaat gtatatctat ttaatctgct
4681 tttcttgtct aataaatata tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt
4741 gtttattttt ttttcttcat tccgtaactc ttctaccttc tttatttact ttctaaaatc
4801 caaatacaaa acataaaaat aaataaacac agagtaaatt cccaaattat tccatcatta
4861 aaagatacga ggcgcgtgta agttacaggc aagcgatccg tcctaagaaa ccattattat
4921 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//