LOCUS Exported 2882 bp ds-DNA circular SYN 10-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2882)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Saturday, September 10, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..2882
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 7..483
/note="GAP promoter"
/note="promoter for Pichia pastoris
glyceraldehyde-3-phosphate dehydrogenase"
CDS 562..591
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
CDS 607..624
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
terminator 703..949
/gene="Pichia pastoris AOX1"
/note="AOX1 terminator"
/note="transcription terminator for AOX1"
promoter 964..1375
/gene="S. cerevisiae TEF1"
/note="TEF1 promoter"
/note="promoter for EF-1-alpha"
promoter 1382..1429
/note="EM7 promoter"
/note="synthetic bacterial promoter "
CDS 1448..1822
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="BleoR"
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
terminator 1888..2135
/gene="S. cerevisiae CYC1"
/note="CYC1 terminator"
/note="transcription terminator for CYC1"
rep_origin complement(2210..2798)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 agatcttttt tgtagaaatg tcttggtgtc ctcgtccaat caggtagcca tctctgaaat
61 atctggctcc gttgcaactc cgaacgacct gctggcaacg taaaattctc cggggtaaaa
121 cttaaatgtg gagtaatgga accagaaacg tctcttccct tctctctcct tccaccgccc
181 gttaccgtcc ctaggaaatt ttactctgct ggagagcttc ttctacggcc cccttgcagc
241 aatgctcttc ccagcattac gttgcgggta aaacggaggt cgtgtacccg acctagcagc
301 ccagggatgg aaaagtcccg gccgtcgctg gcaataatag cgggcggacg catgtcatga
361 gattattgga aaccaccaga atcgaatata aaaggcgaac acctttccca attttggttt
421 ctcctgaccc aaagacttta aatttaattt atttgtccct atttcaatca attgaacaac
481 tatttcgaaa cgaggaattc acgtggccca gccggccgtc tcggatcggt acctcgagcc
541 gcggcggccg ccagctttct agaacaaaaa ctcatctcag aagaggatct gaatagcgcc
601 gtcgaccatc atcatcatca tcattgagtt ttagccttag acatgactgt tcctcagttc
661 aagttgggca cttacgagaa gaccggtctt gctagattct aatcaagagg atgtcagaat
721 gccatttgcc tgagagatgc aggcttcatt tttgatactt ttttatttgt aacctatata
781 gtataggatt ttttttgtca ttttgtttct tctcgtacga gcttgctcct gatcagccta
841 tctcgcagct gatgaatatc ttgtggtagg ggtttgggaa aatcattcga gtttgatgtt
901 tttcttggta tttcccactc ctcttcagag tacagaagat taagtgagac cttcgtttgt
961 gcggatcccc cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat
1021 tttctcggac tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat
1081 tttccctctt tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa
1141 aaaagagacc gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat ttttatcacg
1201 tttctttttc ttgaaatttt tttttttagt ttttttctct ttcagtgacc tccattgata
1261 tttaagttaa taaacggtct tcaatttctc aagtttcagt ttcatttttc ttgttctatt
1321 acaacttttt ttacttcttg ttcattagaa agaaagcata gcaatctaat ctaagggcgg
1381 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
1441 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
1501 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
1561 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
1621 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
1681 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
1741 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
1801 cgtggccgag gagcaggact gacacgtccg acggcggccc acgggtccca ggcctcggag
1861 atccgtcccc cttttccttt gtcgatatca tgtaattagt tatgtcacgc ttacattcac
1921 gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
1981 tccctattta tttttttata gttatgttag tattaagaac gttatttata tttcaaattt
2041 ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg
2101 agaaggtttt gggacgctcg aaggctttaa tttgcaagct ggagaccaac atgtgagcaa
2161 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
2221 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
2281 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
2341 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
2401 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
2461 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
2521 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
2581 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
2641 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
2701 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
2761 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
2821 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcatgaga
2881 tc
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