LOCUS Exported 3760 bp ds-DNA circular SYN 26-SEP-2017
DEFINITION synthetic circular DNA
KEYWORDS ATU6-26-sgRNA-SK
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3760)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 26, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..3760
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 64..168
/gene="bla"
/label=AmpR promoter
CDS 169..1029
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1200..1789
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 2383..2389
/label=U6 terminator
misc_RNA complement(2390..2465)
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_feature complement(2483..2931)
/label=AtU6-26 promoter
/note="Synthetic construct guide RNA, complete sequence
GenBank: KT827739.1"
rep_origin complement(3176..3631)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 ctgatgtcgc gcgcttatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
61 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
121 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
181 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
241 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
301 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
361 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
421 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
481 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
541 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
601 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
661 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
721 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
781 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
841 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
901 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
961 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
1021 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
1081 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
1141 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
1201 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
1261 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
1321 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
1381 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
1441 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
1501 gcgcagcggt cgggctgaac gggggggttc gtgcacacag cccagcttgg agcgaacgac
1561 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
1621 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
1681 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
1741 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
1801 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
1861 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
1921 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
1981 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
2041 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
2101 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
2161 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
2221 tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gaactagtgg
2281 atcccccggg ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagcggcc
2341 gccagtgtga tggatatctg cagaattgcc cttcgaaggg acaaaaaaag caccgactcg
2401 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat ttctagctct
2461 aaaacaggtc ttctcgaaga cccaatcact acttcgactc tagctgtata taaactcagc
2521 ttcgttttct tatctaagcg atgtgggact tttgaagatt gttttcaact taaatgggcc
2581 tatataagaa atactattgt tctttcccat ataaatgggc ctgcttctct tctttcagat
2641 tcccaggggc cttttgaaga ttatcttcat atcttaagaa tgaagatgtt ttattcaatc
2701 aaattcttga aggttcgatg cctaatcatt ctaatcctgg gacaaactat gaaacaagat
2761 acaaaaactc cgaatggaaa gttaaaaaga agaaaacgaa agctacggtt caagaaaatg
2821 taagctgata aacaaaaaaa aactgtatga acgaagaaga agaaaaaaag ctaagaagaa
2881 atgatgtatt gtgcggaagg caagtcgagt ttccgttgtt caacgaagct taagggcaat
2941 tccagcacac tggcggccgt tactagtgga tccgagctcg gtacccaatt cgccctatag
3001 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
3061 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
3121 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt
3181 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
3241 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
3301 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
3361 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
3421 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
3481 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
3541 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
3601 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
3661 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
3721 taacccctga taaatgcttc aataatatga aaaagtaaga
//