LOCUS Exported 3347 bp ds-DNA circular SYN 14-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 10
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3347)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.2.1
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..3347
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 542..564
/note="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA
(truncated)"
promoter 638..982
/note="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 992..1099
/note="MCS"
/note="pBluescript multiple cloning site"
primer_bind 1016..1032
/note="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1066..1082)
/note="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
polyA_signal 1153..1329
/note="CaMV poly(A) signal"
/note="cauliflower mosaic virus polyadenylation signal"
misc_feature 1339..1363
/note="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
rep_origin complement(1454..2042)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2213..3028)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 3319..407
/note="pSa ori"
/note="origin of replication from bacterial plasmid pSa"
ORIGIN
1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
541 ttggcaggat atattgtggt gtaacgttat cagcttggta cgtacccccc tactccaaaa
601 atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
661 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
721 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc
781 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
841 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac atctccactg
901 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
961 gtcatttcat ttggagagga cagcccaagc tgagctccac cgcggtggcg gccgctctag
1021 aactagtgga tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct
1081 cgaggggggg cccggtacca attcggtacg ctgaaatcac cagtctctct ctacaaatct
1141 atctctctct attttctcca taaataatgt gtgagtagtt tcccgataag ggaaattagg
1201 gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta
1261 tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa
1321 atccagatcc actagccttg acaggatata ttggcgggta aactaagtcg ctgtatgtgt
1381 ttgtttgaga tctcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
1441 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
1501 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
1561 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
1621 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
1681 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
1741 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
1801 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
1861 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
1921 tgaagccagt taccttcgga agaagagttg gtagctcttg atccggcaaa caaaccaccg
1981 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
2041 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
2101 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
2161 aatgaagttt taaatcaatc taaagtatat atgtgtaaca ttggtctagt gattagaaaa
2221 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
2281 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
2341 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
2401 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
2461 gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac
2521 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
2581 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
2641 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
2701 atacctggaa tgctgttttc cctgggatcg cagtggtgag taaccatgca tcatcaggag
2761 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
2821 ccatctcatc tgtaacaaca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
2881 gcgcatcggg cttcccatac aatcggtaga ttgtcgcacc tgattgcccg acattatcgc
2941 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggccttgagc
3001 aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag
3061 acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt
3121 gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca
3181 cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact
3241 ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg ctggatgatg
3301 gggcgattca ggcgatcccc atccaacagc ccgccgtcga gcgggct
//