LOCUS Exported 4140 bp ds-DNA circular SYN 01-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..4140
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 225..246
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 261..291
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 299..315
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 323..339
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 863..1208
/note="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 1217..1300
/note="MCS"
CDS 1304..1330
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/note="HA"
/translation="YPYDVPDYA"
misc_feature 1331..1585
/note="YFP-C"
terminator 1602..1854
/note="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(1863..1879)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 2353..2457
/gene="bla"
/note="AmpR promoter"
CDS 2458..3318
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3489..4077
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
61 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
121 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
181 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
241 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
301 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca
361 tgcctgcagg tccccagatt agccttttca atttcagaaa gaatgctaac ccacagatgg
421 ttagagaggc ttacgcagca ggtctcatca agacgatcta cccgagcaat aatctccagg
481 aaatcaaata ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca
541 tcaagaacac agagaaagat atatttctca agatcagaag tactattcca gtatggacga
601 ttcaaggctt gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag
661 ttcccactga atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg
721 ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa
781 tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag
841 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc
901 tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg
961 gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg
1021 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
1081 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
1141 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
1201 agagaacacg ggggactcta gagttaaccg ggctcaggcc tggcgcgcca ctagtggatc
1261 catcgatagt actgtcgacc tcgagggtac cgctcccggg atgtacccat acgatgttcc
1321 agattacgct gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
1381 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
1441 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
1501 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
1561 cggcatggac gagctgtaca agtaagagct cgaatttccc cgatcgttca aacatttggc
1621 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
1681 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
1741 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
1801 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat
1861 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
1921 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
1981 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
2041 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
2101 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
2161 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
2221 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
2281 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
2341 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
2401 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
2461 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
2521 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
2581 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
2641 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
2701 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
2761 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
2821 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
2881 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
2941 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
3001 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
3061 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
3121 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
3181 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
3241 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
3301 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
3361 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
3421 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
3481 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
3541 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
3601 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
3661 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
3721 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
3781 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
3841 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
3901 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
3961 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
4021 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
4081 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
//