LOCUS Exported 12321 bp ds-DNA circular SYN 03-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..12321
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 1..25
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
terminator 111..363
/label=MAS terminator
/note="mannopine synthase terminator"
CDS complement(373..924)
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase"
/label=Zeo
/note="confers resistance to bialophos or phosphinothricin"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
WQLDFSLPVPPRPVLPVTEI"
promoter complement(930..1310)
/label=MAS promoter
/note="mannopine synthase promoter (Velten et al., 1984)"
promoter 2306..2651
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 2681..2735
/label=TMV Omega
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
CDS 2740..2766
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
protein_bind 2770..2894
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 2919..2949
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 3003..3662
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
CDS 4004..4309
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRIVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
protein_bind complement(4350..4474)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS 4494..5012
/codon_start=1
/product="N-terminal fragment of mVenus for use in
bimolecular fluorescence complementation (BiFC) (Kodama and
Hu, 2010)"
/label=VN173
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIE"
terminator 5093..5800
/label=OCS terminator
/note="octopine synthase terminator"
misc_feature 6044..6068
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 7368..7997
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 8431..9498
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
rep_origin 9564..9758
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 10102..10242
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(10428..11016)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(11103..11897)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
2641 tggagaggac acgctcgagt ataagagctc tatttttaca acaattacca acaacaacaa
2701 acaacaaaca acattacaat tacatttaca attaccatgt acccatacga tgttccagat
2761 tacgctatca caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc
2821 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
2881 atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct
2941 cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta
3001 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
3061 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
3121 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
3181 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
3241 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
3301 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
3361 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
3421 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
3481 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
3541 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
3601 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
3661 aaacgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
3721 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc
3781 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
3841 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
3901 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
3961 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta
4021 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
4081 cacgcccggg cgacggatag tgatccccct ggccagtgca cgtctgctgt cagataaagt
4141 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
4201 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
4261 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
4321 ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt
4381 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
4441 acgtttctcg ttcagctttc ttgtacaaag tggtgcctag gagtatcgct acaatggtga
4501 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
4561 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
4621 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
4681 ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
4741 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
4801 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
4861 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
4921 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
4981 aggtgaactt caagatccgc cacaacatcg aggactgacc taggtgagtc tagagagtta
5041 atctaggtga gtctagagag ttaattaaga cccgggacta gtccctagag tcctgcttta
5101 atgagatatg cgagacgcct atgatcgcat gatatttgct ttcaattctg ttgtgcacgt
5161 tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc cggtttcggt tcattctaat
5221 gaatatatca cccgttacta tcgtattttt atgaataata ttctccgttc aatttactga
5281 ttgtacccta ctacttatat gtacaatatt aaaatgaaaa caatatattg tgctgaatag
5341 gtttatagcg acatctatga tagagcgcca caataacaaa caattgcgtt ttattattac
5401 aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct aaaagactga ttacataaat
5461 cttattcaaa tttcaaaagt gccccagggg ctagtatcta cgacacaccg agcggcgaac
5521 taataacgct cactgaaggg aactccggtt ccccgccggc gcgcatgggt gagattcctt
5581 gaagttgagt attggccgtc cgctctaccg aaagttacgg gcaccattca acccggtcca
5641 gcacggcggc cgggtaaccg acttgctgcc ccgagaatta tgcagcattt ttttggtgta
5701 tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg acgacaaatc gttgggcggg
5761 tccagggcga attttgcgac aacatgtcga ggctcagcag gacctgcagg catgcaagct
5821 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
5881 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
5941 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg
6001 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
6061 gggtaaacct aagagaaaag agcgtttatt agaataacgg atatttaaaa gggcgtgaaa
6121 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
6181 aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca
6241 gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct
6301 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga
6361 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc
6421 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg
6481 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc
6541 caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct
6601 ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg
6661 cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt
6721 gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag
6781 cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc
6841 ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc
6901 ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga
6961 agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc
7021 cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag
7081 gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc
7141 gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc
7201 ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag
7261 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct
7321 gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg
7381 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg
7441 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc
7501 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga
7561 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc
7621 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg
7681 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc
7741 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag
7801 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg
7861 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc
7921 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta
7981 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct
8041 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga
8101 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa
8161 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca
8221 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag
8281 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag
8341 gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg
8401 gaacccccaa gcccgaggaa tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc
8461 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
8521 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
8581 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
8641 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
8701 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
8761 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
8821 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
8881 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
8941 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
9001 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
9061 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
9121 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
9181 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
9241 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
9301 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
9361 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
9421 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
9481 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
9541 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
9601 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
9661 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
9721 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
9781 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
9841 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
9901 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
9961 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
10021 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
10081 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
10141 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
10201 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
10261 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
10321 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
10381 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
10441 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
10501 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
10561 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
10621 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
10681 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
10741 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
10801 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
10861 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
10921 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
10981 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
11041 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg
11101 tactaaaaca attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc
11161 cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac
11221 cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata
11281 aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa
11341 aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct
11401 ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt
11461 aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg
11521 tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt
11581 tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg
11641 ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc
11701 catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc
11761 gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac
11821 attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat
11881 attctcattt tagccattta ttatttcctt cctcttttct acagtattta aagatacccc
11941 aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta
12001 aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga
12061 cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc
12121 aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct
12181 tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac
12241 gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
12301 ggggagctgt tggctggctg g
//