LOCUS Exported 2723 bp ds-DNA circular SYN 17-AUG-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pENTR3C
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2723)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Wednesday, August 17, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..2723
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 103..189
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 281..308
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
protein_bind 358..457
/gene="mutant version of attL"
/bound_moiety="LR Clonase(TM)"
/note="attL1"
/note="recombination site for the Gateway(R) LR reaction"
CDS 618..923
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/note="ccdB"
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
protein_bind complement(952..1051)
/gene="mutant version of attL"
/bound_moiety="LR Clonase(TM)"
/note="attL2"
/note="recombination site for the Gateway(R) LR reaction"
CDS 1174..1983
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 2073..2661
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
421 ttttttataa tgccaacttt gtacaaaaaa gcaggctctt taaaggaacc aattcagtcg
481 actggatccg gtaccgaatt cgatcgctta ctaaaagcca gataacagta tgcgtatttg
541 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
601 agaggtgtgc ttctagaatg cagtttaagg tttacaccta taaaagagag agccgttatc
661 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
721 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
781 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
841 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
901 acctgatgtt ctggggaata tagaattcgc ggccgcactc gagatatcta gacccagctt
961 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg
1021 tcactatcag tcaaaataaa atcattattt gccatccagc tgcagctctg gcccgtgtct
1081 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
1141 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
1201 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
1261 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
1321 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
1381 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
1441 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
1501 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
1561 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
1621 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
1681 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
1741 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
1801 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
1861 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
1921 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
1981 taatcagaat tggttaattg gttgtaacat tattcagatt gggccccgtt ccactgagcg
2041 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
2101 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
2161 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
2221 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
2281 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
2341 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
2401 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
2461 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
2521 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
2581 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
2641 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
2701 tgctggcctt ttgctcacat gtt
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