LOCUS Exported 4530 bp ds-DNA circular SYN 13-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4530)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, September 13, 2016 from SnapGene Viewer 3.2.1
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4530
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 512..857
/note="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 903..1622
/codon_start=1
/product="enhanced GFP"
/note="EGFP"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITHGMDELYK"
terminator 1641..1893
/note="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(1902..1918)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 2392..2496
/gene="bla"
/note="AmpR promoter"
CDS 2497..3357
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3528..4116
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 4404..4425
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4440..4470
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 4478..4494
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4502..4518
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
61 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
121 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
181 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
241 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
301 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
361 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
421 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
481 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
541 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
601 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
661 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
721 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
781 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
841 ttcatttgga gagaacacgg gggactctag aggatccgaa ttcgagctcc gtcgacaagc
901 ttatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
961 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
1021 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
1081 ccctcgtgac caccttcacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
1141 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
1201 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
1261 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
1321 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
1381 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
1441 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
1501 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
1561 tcctgctgga gttcgtgacc gccgccggga tcactcacgg catggacgag ctgtacaagt
1621 aaagcggccc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
1681 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
1741 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
1801 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
1861 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt cactggccgt cgttttacaa
1921 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
1981 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
2041 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
2101 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
2161 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
2221 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
2281 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
2341 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
2401 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
2461 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
2521 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
2581 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
2641 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
2701 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
2761 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
2821 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
2881 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
2941 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
3001 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
3061 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
3121 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
3181 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
3241 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
3301 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
3361 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
3421 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
3481 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
3541 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
3601 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
3661 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
3721 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
3781 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
3841 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
3901 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
3961 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
4021 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
4081 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
4141 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
4201 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
4261 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
4321 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
4381 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
4441 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
4501 acaggaaaca gctatgacca tgattacgcc
//