LOCUS Exported 13832 bp ds-DNA circular SYN 13-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13832)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, September 13, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..13832
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(145..516)
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/note="traJ"
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
oriT 549..658
/note="incP origin of transfer"
CDS complement(1339..1989)
/codon_start=1
/gene="tetR"
/product="tetracycline resistance regulatory protein"
/note="TetR"
/translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
misc_feature 2454..2478
/note="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
promoter 2602..2781
/note="NOS promoter"
/note="nopaline synthase promoter"
promoter 2634..2817
/function="NOS promoter"
/note="NOS promoter"
/note="nopaline synthase promoter"
CDS 2838..3632
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
terminator 4022..4274
/note="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
protein_bind 4823..4844
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4859..4889
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 4897..4913
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4921..4937
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 5461..5805
/note="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 5962..5991
/codon_start=1
/product="10xHis affinity tag"
/note="10xHis"
/translation="HHHHHHHHHH"
CDS 6013..6729
/codon_start=1
/product="enhanced GFP"
/note="EGFP"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITHGMDELDK"
CDS 6748..6765
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
terminator 6801..7053
/note="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(7062..7078)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 7696..7720
/note="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
CDS complement(9316..10464)
/codon_start=1
/product="trans-acting replication protein that binds to
and activates oriV"
/note="trfA"
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
CDS complement(10763..11557)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
mobile_element 11749..12516
/mobile_element_type="insertion sequence:IS1"
/note="IS1"
/note="prokaryotic transposable element"
rep_origin 13139..18
/note="oriV"
/note="incP origin of replication"
ORIGIN
1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
5701 ggattgatgt gatatctcca atgacgtaag ggatgacgca caatcccact atccttcgca
5761 agacccttcc tctatataag gaagttcatt tcatttggag aggacctcga cctcaacaca
5821 acatatacaa aacaaacgaa tctcaagcaa tcaagcattc tacttctatt gcagcaattt
5881 aaatcatttc ttttaaagca aaagcaattt tctgaaaatt ttcaccattt acgaacgata
5941 ctcgagatga cccgtggttc tcatcaccat caccatcacc atcaccatca cgccatggtc
6001 gacgtactag tgatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
6061 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
6121 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
6181 ccctggccca ccctcgtgac caccttcacc tacggcgtgc agtgcttcag ccgctacccc
6241 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
6301 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
6361 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
6421 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
6481 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
6541 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
6601 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
6661 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactcacgg catggacgag
6721 ctggacaagg gtctagaccc gggaatgcat caccatcacc atcacggatc ctgattgatc
6781 gatagagctc gaatttcccc gatcgatcaa acatttggca ataaagtttc ttaagattga
6841 atcctgttgc cgggcttgcg atgattatca tataatttct gttgaattac gttaaacatg
6901 aaataattaa catgtaatgc atgacgttat ttatgatatg ggtttttatg attatagtcc
6961 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac tacgataaat
7021 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt cactggccgt cgttttacaa
7081 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
7141 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
7201 agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
7261 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
7321 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
7381 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
7441 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
7501 gccgatttcg gaaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
7561 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
7621 ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc cgcaatgtgt tattaagttg
7681 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
7741 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
7801 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
7861 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
7921 tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc ctcgcagaga
7981 tccgaattat cagccttctt attcatttct cgcttaaccg tgacaggctg tcgatcttga
8041 gaactatgcc gacataatag gaaatcgctg gataaagccg ctgaggaagc tgagtggcgc
8101 tatttcttta gaagtgaacg ttgacgatat caactcccct atccattgct caccgaatgg
8161 tacaggtcgg ggacccgaag ttccgactgt cggcctgatg catccccggc tgatcgaccc
8221 cagatctggg gctgagaaag cccagtaagg aaacaactgt aggttcgagt cgcgagatcc
8281 cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac gccaggccga
8341 gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct actggaacga
8401 gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc acgggaggtt
8461 gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc aggcccgctg
8521 cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc acgcccgcag
8581 ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc agagcggcag
8641 agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc ccgcccggca
8701 ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt gcgccgagga
8761 tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc acgagcaata
8821 aacccgccgg caacgcccgc agcagcatac cggcgacccc tcggcctcgc tgttcgggct
8881 ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc tcctgtttga
8941 agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg gcatctcgca
9001 accgttcagc gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg gacccgaaca
9061 tctctggagc tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc acgtcggccg
9121 ctccaagccg tcgaatctga gccttaatca caattgtcaa ttttaatcct ctgtttatcg
9181 gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag tgcgtcgagc
9241 agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc cagccggaac
9301 tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc aggcgtgttc
9361 caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc gccacttctt
9421 cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga gcgggtacgg
9481 ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta
9541 cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga tttcctcgtc
9601 gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga agcggtgcag
9661 cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg
9721 taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg accagcccag
9781 gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc
9841 gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct cgacgccggt
9901 gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg
9961 cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc
10021 tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt ccttgatctg
10081 ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc
10141 ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga tggtcatcga
10201 cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg cggccgatgg
10261 cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg
10321 ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga cggtgcggct
10381 tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc
10441 cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga ctcacgccgg
10501 ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca gataatccac
10561 cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt acttggtatt
10621 ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc cgtgggcctc
10681 ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc
10741 atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa tattttattt
10801 tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc
10861 cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc
10921 cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt
10981 ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat
11041 catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat
11101 cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg
11161 tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct
11221 cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg
11281 cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa
11341 caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg
11401 tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct
11461 tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca
11521 gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct
11581 acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt
11641 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
11701 ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg tgatgctgcc
11761 aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtgtctat
11821 cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg
11881 acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt tcacttacac
11941 cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg gcgttggatg
12001 ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg attttacgtc acttaaaaaa
12061 ctcaggccgc agtcggtaac ctcgcgcata cagccgggca gtgacgtcat cgtctgcgcg
12121 gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg gctgttttac
12181 gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat tcggtgaacg cactatggcg
12241 acgctggggc gtcttatgag cctgctgtca ccctttgacg tggtgatatg gatgacggat
12301 ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag caagcgatat
12361 acgcagcgaa ttgagcggca taacctgaat ctgaggcagc acctggcacg gctgggacgg
12421 aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg gcattatctg
12481 aacataaaac actatcaata agttggagtc attacccaat tatgatagaa tttacaagct
12541 ataaggttat tgtcctgggt ttcaagcatt agtccatgca agtttttatg ctttgcccat
12601 tctatagata tattgataag cgcgctgcct atgccttgcc ccctgaaatc cttacatacg
12661 gcgatatctt ctatataaaa gatatattat cttatcagta ttgtcaatat attcaaggca
12721 atctgcctcc tcatcctctt catcctcttc gtcttggtag ctttttaaat atggcgcttc
12781 atagagtaat tctgtaaagg tccaattctc gttttcatac ctcggtataa tcttacctat
12841 cacctcaaat ggttcgctgg gtttatcgca cccccgaaca cgagcacggc acccgcgacc
12901 actatgccaa gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc cgtgaatgcc
12961 ccgacggccg aagtgaaggg caggccgcca cccaggccgc cgccctcact gcccggcacc
13021 tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa tgcttccggg cgtcgcgctc
13081 gggctgatcg cccatcccgt tactgccccg atcccggcaa tggcaaggac tgccagcgct
13141 gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg
13201 cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg
13261 tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc
13321 aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga
13381 ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact
13441 ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg
13501 tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta
13561 aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg
13621 agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc
13681 cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc
13741 gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc
13801 cgccagccca gcggcgaggg caaccagccc gg
//