LOCUS Exported 3288 bp ds-DNA circular SYN 25-OCT-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3288)
TITLE Direct Submission
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..3288
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_signal 190..340
/label=Ad5 Psi
/note="packaging signal for adenovirus serotype 5"
misc_feature 455..508
/label=MCS
protein_bind 526..559
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
rep_origin complement(1022..1610)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1781..2641)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2642..2746)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
421 gggtcaaagt tggcgtttta ttattatagt cagctctaga gtcgacgagc tcaagcttag
481 atctgctagc aggcctgaat tcggatccct cgactcgaag atccaataac ttcgtatagc
541 atacattata cgaagttata agtgccgccc tataccttgt ctgcctcccc gcgttgcgtc
601 gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg
661 attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac
721 caacccttgg cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat
781 ctcggctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
841 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
901 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
961 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
1021 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
1081 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
1141 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
1201 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
1261 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
1321 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
1381 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
1441 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
1501 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
1561 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
1621 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
1681 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
1741 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
1801 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1861 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1921 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1981 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
2041 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
2101 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
2161 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
2221 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
2281 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
2341 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
2401 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
2461 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
2521 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
2581 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
2641 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
2701 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
2761 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
2821 gtatcactct aggcaaaata gcaccctccc gctccagaac aacatacagc gcttcacagc
2881 ggcagcctaa cagtcagcct taccagtaaa aaagaaaacc tattaaaaaa acaccactcg
2941 acacggcacc agctcaatca gtcacagtgt aaaaaagggc caagtgcaga gcgagtatat
3001 ataggactaa aaaatgacgt aacggttaaa gtccacaaaa aacacccaga aaaccgcacg
3061 cgaacctacg cccagaaacg aaagccaaaa aacccacaac ttcctcaaat cgtcacttcc
3121 gttttcccac gttacgtaac ttcccatttt aagaaaacta caattcccaa cacatacaag
3181 ttactccgcc ctaaaaccta cgtcacccgc cccgttccca cgccccgcgc cacgtcacaa
3241 actccacccc ctcattatca tattggcttc aatccaaaat aaggtata
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