LOCUS Exported 5879 bp ds-DNA circular SYN 10-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..5879
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 468..992
/label=mCMV promoter
CDS 999..1715
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
polyA_signal 1862..1983
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
enhancer 1991..2370
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2371..2574
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 2619..2637
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 2651..2740
/label=MCS
polyA_signal 2764..2988
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
polyA_signal 3002..3136
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind 3156..3189
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
rep_origin complement(3613..4201)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4372..5232)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5233..5337)
/gene="bla"
/label=AmpR promoter
misc_feature 5420..453
/label=Ad
ORIGIN
1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
421 gggtcaaagt tggcgtttta ttattatagt cagtctagag atatactgag tcattaggga
481 ctttccaatg ggttttgccc agtacataag gtcaataggg gtgaatcaac aggaaagtcc
541 cattggagcc aagtacactg agtcaatagg gactttccat tgggttttgc ccagtacaaa
601 aggtcaatag ggggtgagtc aatgggtttt tcccattatt ggcacgtaca taaggtcaat
661 aggggtgagt cattgggttt ttccagccaa tttaattaaa acgccatgta ctttcccacc
721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat
781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg
841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cacgcattct
901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca
961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcat ggtgagcaag ggcgaggagc
1021 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
1081 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
1141 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
1201 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
1261 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
1321 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
1381 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
1441 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
1501 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
1561 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
1621 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
1681 ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct cgagatccac
1741 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
1801 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
1861 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
1921 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
1981 ttaacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
2041 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
2101 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
2161 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
2221 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
2281 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
2341 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
2401 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
2461 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
2521 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
2581 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
2641 acccaagctg gctagcgttt aaacgggccc tctagactcg agcggccgcc actgtgctgg
2701 atatctgcag aattgatccg agctcggtac caagcttaag tttaaaccgc tgatcagcct
2761 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
2821 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
2881 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
2941 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatgggt cgacttcgag
3001 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
3061 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
3121 ttatcatgtc tggatcgtct agcatcgaag atccaataac ttcgtatagc atacattata
3181 cgaagttata agtagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
3241 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
3301 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
3361 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
3421 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
3481 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
3541 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
3601 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
3661 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
3721 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
3781 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
3841 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
3901 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
3961 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
4021 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
4081 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
4141 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
4201 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
4261 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
4321 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
4381 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
4441 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
4501 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
4561 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
4621 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
4681 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
4741 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
4801 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
4861 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
4921 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
4981 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
5041 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
5101 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
5161 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
5221 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
5281 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
5341 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
5401 taaaaatagg cgtatcactc taggcaaaat agcaccctcc cgctccagaa caacatacag
5461 cgcttcacag cggcagccta acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa
5521 aacaccactc gacacggcac cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag
5581 agcgagtata tataggacta aaaaatgacg taacggttaa agtccacaaa aaacacccag
5641 aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa
5701 tcgtcacttc cgttttccca cgttacgtaa cttcccattt taagaaaact acaattccca
5761 acacatacaa gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg
5821 ccacgtcaca aactccaccc cctcattatc atattggctt caatccaaaa taaggtata
//