LOCUS Exported 6679 bp ds-DNA circular SYN 19-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..6679
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter complement(1586..1789)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer 1790..2093
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
rep_origin complement(2685..3273)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3444..4304)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4305..4409)
/gene="bla"
/label=AmpR promoter
polyA_signal 4497..4578
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS complement(4808..5407)
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
promoter complement(5504..5820)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5518..5653
/label=SV40 ori
/note="SV40 origin of replication"
misc_feature 6235..6293
/label=CMV pA signal
misc_feature 6299..1564
/label=Stuffer
ORIGIN
1 tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga
61 acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt
121 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
181 aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact
241 cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga
301 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
361 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
421 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc
481 aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa
541 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc
601 ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc
661 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
721 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
781 ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta
841 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
901 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc
961 tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct
1021 tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg
1081 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
1141 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
1201 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
1261 tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta attctagagt cggggcggcc
1321 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
1381 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
1441 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
1501 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
1561 ataaggatcc acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc
1621 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat
1681 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg
1741 agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag
1801 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
1861 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
1921 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
1981 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
2041 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
2101 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctaagatctg
2161 gtaccttgaa ttcatgcttc tcctcccttt agtgagggta attctctctc tctccctata
2221 gtgagtcgta ttaattcctt ctcttctata gtgtcaccta aatcgttgca attcgtaatc
2281 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
2341 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
2401 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
2461 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
2521 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
2581 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
2641 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
2701 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
2761 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
2821 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
2881 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
2941 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
3001 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
3061 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
3121 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
3181 ggtagctctt gatccggcaa aaaaaccacc gctggtagcg gtggtttttt tgtttgcaag
3241 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
3301 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
3361 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
3421 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
3481 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
3541 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
3601 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
3661 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
3721 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
3781 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
3841 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
3901 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
3961 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
4021 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
4081 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
4141 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
4201 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
4261 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
4321 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
4381 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
4441 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gagattgcag
4501 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
4561 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
4621 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
4681 gatcctctag agtcggtggg cctcgggggc gggtgcgggg tcggcggggc cgccccgggt
4741 ggcttcggtc ggagccatgg ggtcgtgcgc tcctttcggt cgggcgctgc gggtcgtggg
4801 gcgggcgtca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct
4861 cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg
4921 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga
4981 cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg gccaggaacc
5041 acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca
5101 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt
5161 cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct
5221 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga
5281 tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga
5341 gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact
5401 cggtcatgga aggtcgtctc cttgtgaggg gtcaggggcg tgggtcaggg gatggtggcg
5461 gcaccggtcg tggcggccga cctgcaggca tgcaagctag ctttttgcaa aagcctaggc
5521 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
5581 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag
5641 ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca
5701 tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact
5761 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
5821 aatctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
5881 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
5941 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
6001 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
6061 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
6121 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
6181 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctagcggc
6241 cgcatacaaa aaaccaacac acagatccaa tgaaaataaa agatccttta ttaagcttgg
6301 cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa gaaaggcccg
6361 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag
6421 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc
6481 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
6541 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
6601 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
6661 cgtgaattgc tcaacagta
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