LOCUS Exported 5659 bp ds-DNA circular SYN 04-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5659)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sunday, September 4, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..5659
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 96..127
/note="minP"
/note="minimal TATA-box promoter with low basal activity"
CDS 160..1812
/codon_start=1
/product="firefly luciferase"
/note="luciferase"
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
polyA_signal 1856..1977
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 2171..2528
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2379..2514
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2559..3353
/codon_start=1
/product="aminoglycoside phosphotransferase"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3378..3426
/note="synthetic polyadenylation signal"
rep_origin complement(3753..4341)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4541..5401)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 5506..5554
/note="synthetic polyadenylation signal"
misc_feature 5568..5659
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacca cgtttgcctt gcctggactt gcctggcctt gccttggaca
61 tgcccgggct gtcagatctg cagaagctta gacactagag ggtatataat ggaagctcga
121 cttccagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt
181 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
241 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
301 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
361 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
421 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
481 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
541 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
601 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
661 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
721 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
781 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
841 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
901 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
961 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
1021 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
1081 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
1141 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
1201 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
1261 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
1321 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
1381 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
1441 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
1501 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
1561 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
1621 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca
1681 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc
1741 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag
1801 atcgccgtgt aataattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
1861 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
1921 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
1981 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
2041 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtttgc gtattgggcg
2101 ctcttccgct gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
2161 tagctacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
2221 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
2281 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
2341 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
2401 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
2461 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
2521 tttgcaaaaa gctcgattct tctgacacta gcgccaccat gatcgaacaa gacggcctcc
2581 atgctggcag tcccgcagct tgggtcgaac gcttgttcgg gtacgactgg gcccagcaga
2641 ccatcggatg tagcgatgcg gccgtgttcc gtctaagcgc tcaaggccgg cccgtgctgt
2701 tcgtgaagac cgacctgagc ggcgccctga acgagcttca agacgaggct gcccgcctga
2761 gctggctggc caccaccggc gtaccctgcg ccgctgtgtt ggatgttgtg accgaagccg
2821 gccgggactg gctgctgctg ggcgaggtcc ctggccagga tctgctgagc agccaccttg
2881 cccccgctga gaaggtttct atcatggccg atgcaatgcg gcgcctgcac accctggacc
2941 ccgctacctg ccccttcgac caccaggcta agcatcggat cgagcgtgct cggacccgca
3001 tggaggccgg cctggtggac caggacgacc tggacgagga gcatcagggc ctggcccccg
3061 ctgaactgtt cgcccgactg aaagcccgca tgccggacgg tgaggacctg gttgtcacac
3121 acggagatgc ctgcctccct aacatcatgg tcgagaatgg ccgcttctcc ggcttcatcg
3181 actgcggtcg cctaggagtt gccgaccgct accaggacat cgccctggcc acccgcgaca
3241 tcgctgagga gcttggcggc gagtgggccg accgcttctt agtcttgtac ggcatcgcag
3301 ctcccgacag ccagcgcatc gccttctacc gcttgctcga cgagttcttt taatgatcta
3361 gaaccggtca tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt
3421 tgtgtgttcg aactagatgc tgtcgaccga tgcccttgag agccttcaac ccagtcagct
3481 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
3541 tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg
3601 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
3661 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
3721 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
3781 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
3841 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
3901 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
3961 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
4021 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
4081 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
4141 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
4201 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
4261 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
4321 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
4381 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
4441 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
4501 tggtctgaca gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc ttgatcagtg
4561 aggcaccgat ctcagcgatc tgcctatttc gttcgtccat agtggcctga ctccccgtcg
4621 tgtagatcac tacgattcgt gagggcttac catcaggccc cagcgcagca atgatgccgc
4681 gagagccgcg ttcaccggcc cccgatttgt cagcaatgaa ccagccagca gggagggccg
4741 agcgaagaag tggtcctgct actttgtccg cctccatcca gtctatgagc tgctgtcgtg
4801 atgctagagt aagaagttcg ccagtgagta gtttccgaag agttgtggcc attgctactg
4861 gcatcgtggt atcacgctcg tcgttcggta tggcttcgtt caactctggt tcccagcggt
4921 caagccgggt cacatgatca cccatattat gaagaaatgc agtcagctcc ttagggcctc
4981 cgatcgttgt cagaagtaag ttggccgcgg tgttgtcgct catggtaatg gcagcactac
5041 acaattctct taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc gagtactcaa
5101 ccaagtcgtt ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg gcgtctatac
5161 gggacaacac cgcgccacat agcagtactt tgaaagtgct catcatcggg aatcgttctt
5221 cggggcggaa agactcaagg atcttgccgc tattgagatc cagttcgata tagcccactc
5281 ttgcacccag ttgatcttca gcatctttta ctttcaccag cgtttcgggg tgtgcaaaaa
5341 caggcaagca aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt tggatgctca
5401 tactcgtcct ttttcaatat tattgaagca tttatcaggg ttactagtac gtctctcaag
5461 gataagtaag taatattaag gtacgggagg tattggacag gccgcaataa aatatcttta
5521 ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc
5581 catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca
5641 ggtgccagaa catttctct
//