LOCUS Exported 4879 bp ds-DNA circular SYN 26-JUL-2013
DEFINITION Promoterless vector for measuring the activity of promoter and
enhancer sequences with a Renilla luciferase assay.
ACCESSION DQ188843
VERSION .
KEYWORDS pGL4.79[hRluc Neo]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4879)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Sunday, September 4, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4879
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
misc_feature 1..70
/note="MCS"
/note="multiple cloning site"
CDS 100..1035
/codon_start=1
/product="Renilla luciferase"
/note="hRluc"
/note="human codon-optimized"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
polyA_signal 1076..1197
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 1391..1748
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 1599..1734
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 1779..2573
/codon_start=1
/product="aminoglycoside phosphotransferase"
/note="NeoR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 2598..2646
/note="synthetic polyadenylation signal"
rep_origin complement(2973..3561)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3761..4621)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 4726..4774
/note="synthetic polyadenylation signal"
misc_feature 4788..4879
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
1021 aagaacgagc agtaattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
1081 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
1141 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
1201 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
1261 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtttgc gtattgggcg
1321 ctcttccgct gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
1381 tagctacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
1441 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
1501 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
1561 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
1621 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
1681 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
1741 tttgcaaaaa gctcgattct tctgacacta gcgccaccat gatcgaacaa gacggcctcc
1801 atgctggcag tcccgcagct tgggtcgaac gcttgttcgg gtacgactgg gcccagcaga
1861 ccatcggatg tagcgatgcg gccgtgttcc gtctaagcgc tcaaggccgg cccgtgctgt
1921 tcgtgaagac cgacctgagc ggcgccctga acgagcttca agacgaggct gcccgcctga
1981 gctggctggc caccaccggc gtaccctgcg ccgctgtgtt ggatgttgtg accgaagccg
2041 gccgggactg gctgctgctg ggcgaggtcc ctggccagga tctgctgagc agccaccttg
2101 cccccgctga gaaggtttct atcatggccg atgcaatgcg gcgcctgcac accctggacc
2161 ccgctacctg ccccttcgac caccaggcta agcatcggat cgagcgtgct cggacccgca
2221 tggaggccgg cctggtggac caggacgacc tggacgagga gcatcagggc ctggcccccg
2281 ctgaactgtt cgcccgactg aaagcccgca tgccggacgg tgaggacctg gttgtcacac
2341 acggagatgc ctgcctccct aacatcatgg tcgagaatgg ccgcttctcc ggcttcatcg
2401 actgcggtcg cctaggagtt gccgaccgct accaggacat cgccctggcc acccgcgaca
2461 tcgctgagga gcttggcggc gagtgggccg accgcttctt agtcttgtac ggcatcgcag
2521 ctcccgacag ccagcgcatc gccttctacc gcttgctcga cgagttcttt taatgatcta
2581 gaaccggtca tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt
2641 tgtgtgttcg aactagatgc tgtcgaccga tgcccttgag agccttcaac ccagtcagct
2701 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
2761 tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg
2821 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
2881 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
2941 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
3001 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
3061 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
3121 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
3181 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
3241 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
3301 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
3361 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
3421 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
3481 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
3541 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
3601 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
3661 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
3721 tggtctgaca gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc ttgatcagtg
3781 aggcaccgat ctcagcgatc tgcctatttc gttcgtccat agtggcctga ctccccgtcg
3841 tgtagatcac tacgattcgt gagggcttac catcaggccc cagcgcagca atgatgccgc
3901 gagagccgcg ttcaccggcc cccgatttgt cagcaatgaa ccagccagca gggagggccg
3961 agcgaagaag tggtcctgct actttgtccg cctccatcca gtctatgagc tgctgtcgtg
4021 atgctagagt aagaagttcg ccagtgagta gtttccgaag agttgtggcc attgctactg
4081 gcatcgtggt atcacgctcg tcgttcggta tggcttcgtt caactctggt tcccagcggt
4141 caagccgggt cacatgatca cccatattat gaagaaatgc agtcagctcc ttagggcctc
4201 cgatcgttgt cagaagtaag ttggccgcgg tgttgtcgct catggtaatg gcagcactac
4261 acaattctct taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc gagtactcaa
4321 ccaagtcgtt ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg gcgtctatac
4381 gggacaacac cgcgccacat agcagtactt tgaaagtgct catcatcggg aatcgttctt
4441 cggggcggaa agactcaagg atcttgccgc tattgagatc cagttcgata tagcccactc
4501 ttgcacccag ttgatcttca gcatctttta ctttcaccag cgtttcgggg tgtgcaaaaa
4561 caggcaagca aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt tggatgctca
4621 tactcgtcct ttttcaatat tattgaagca tttatcaggg ttactagtac gtctctcaag
4681 gataagtaag taatattaag gtacgggagg tattggacag gccgcaataa aatatcttta
4741 ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc
4801 catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca
4861 ggtgccagaa catttctct
//