LOCUS Exported 4733 bp ds-DNA circular SYN 20-JUN-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Cerulean-N1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4733)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Thursday, September 1, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4733
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 16..319
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 320..523
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 546..626
/note="MCS"
/note="multiple cloning site"
CDS 634..1353
/codon_start=1
/product="enhanced monomeric variant of CFP (Rizzo et al.,
2004)"
/note="mCerulean"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
polyA_signal 1476..1597
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1604..2059)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2086..2190
/gene="bla"
/note="AmpR promoter"
promoter 2192..2549
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2400..2535
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2584..3378
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3610..3657
/note="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 3986..4574
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
541 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgacg
601 gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc
661 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
721 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
781 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg
841 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
901 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
961 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
1021 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacgc catcagcgac
1081 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
1141 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
1201 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
1261 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
1321 atcactctcg gcatggacga gctgtacaag taaagcggcc gcgactctag atcataatca
1381 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
1441 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
1501 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
1561 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat
1621 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
1681 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
1741 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
1801 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
1861 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
1921 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
1981 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
2041 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
2101 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
2161 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg
2221 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
2281 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
2341 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
2401 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
2461 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
2521 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt
2581 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
2641 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
2701 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
2761 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
2821 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
2881 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
2941 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
3001 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
3061 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
3121 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
3181 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
3241 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
3301 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
3361 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
3421 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
3481 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
3541 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
3601 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata
3661 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
3721 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
3781 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata
3841 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
3901 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
3961 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
4021 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
4081 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
4141 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
4201 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
4261 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
4321 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
4381 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
4441 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
4501 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
4561 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
4621 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
4681 ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag ccc
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